LOCUS       40924_19896        5251 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle cosmid vector pFD666, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Auerswald EA, Ludwig G, Schaller H.
  TITLE     Structural analysis of Tn5
  JOURNAL   Cold Spring Harb. Symp. Quant. Biol. 45 (Pt 1), 107-113 (1981)
  PUBMED    6271452
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Beck E, Ludwig G, Auerswald EA, Reiss B, Schaller H.
  TITLE     Nucleotide sequence and exact localization of the neomycin 
            phosphotransferase gene from transposon Tn5
  JOURNAL   Gene 19 (3), 327-336 (1982)
  PUBMED    6295884
REFERENCE   3  (bases 1 to 5251)
  AUTHORS   Norrander J, Kempe T, Messing J.
  TITLE     Construction of improved M13 vectors using 
            oligodeoxynucleotide-directed mutagenesis
  JOURNAL   Gene 26 (1), 101-106 (1983)
  PUBMED    6323249
REFERENCE   4  (bases 1 to 5251)
  AUTHORS   Poustka A, Rackwitz HR, Frischauf AM, Hohn B, Lehrach H.
  TITLE     Selective isolation of cosmid clones by homologous recombination in 
            Escherichia coli
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 81 (13), 4129-4133 (1984)
  PUBMED    6330743
REFERENCE   5  (bases 1 to 5251)
  AUTHORS   Yanisch-Perron C, Vieira J, Messing J.
  TITLE     Improved M13 phage cloning vectors and host strains: nucleotide 
            sequences of the M13mp18 and pUC19 vectors
  JOURNAL   Gene 33 (1), 103-119 (1985)
  PUBMED    2985470
REFERENCE   6  (bases 1 to 5251)
  AUTHORS   Gibson TJ, Rosenthal A, Waterston RH.
  TITLE     Lorist6, a cosmid vector with BamHI, NotI, ScaI and HindIII cloning 
            sites and altered neomycin phosphotransferase gene expression
  JOURNAL   Gene 53 (2-3), 283-286 (1987)
  PUBMED    3038694
REFERENCE   7  (bases 1 to 5251)
  AUTHORS   Gibson TJ, Coulson AR, Sulston JE, Little PF.
  TITLE     Lorist2, a cosmid with transcriptional terminators insulating vector
            genes from interference by promoters within the insert: effect on 
            DNA yield and cloned insert frequency
  JOURNAL   Gene 53 (2-3), 275-281 (1987)
  PUBMED    3301536
REFERENCE   8  (bases 1 to 5251)
  AUTHORS   Denis F, Brzezinski R.
  TITLE     An improved aminoglycoside resistance gene cassette for use in 
            gram-negative bacteria and Streptomyces
  JOURNAL   FEMS Microbiol. Lett. 65 (3), 261-264 (1991)
  PUBMED    1655560
REFERENCE   9  (bases 1 to 5251)
  AUTHORS   Denis F, Brzezinski R.
  TITLE     A versatile shuttle cosmid vector for use in Escherichia coli and 
            actinomycetes
  JOURNAL   Gene 111 (1), 115-118 (1992)
  PUBMED    1547947
REFERENCE   10  (bases 1 to 5251)
  AUTHORS   Servin-Gonzalez L, Sampieri AI, Cabello J, Galvan L, Juarez V, 
            Castro C.
  TITLE     Sequence and functional analysis of the Streptomyces 
            phaeochromogenes plasmid pJV1 reveals a modular organization of 
            Streptomyces plasmids that replicate by rolling circle
  JOURNAL   Microbiology 141 (Pt 10), 2499-2510 (1995)
  PUBMED    7582009
REFERENCE   11  (bases 1 to 5251)
  AUTHORS   Denis F, Brzezinski R.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-1997) Immunology, IRCM, 110 Pine Avenue West, 
            Montreal, QC H2W 1R7, Canada
REFERENCE   12  (bases 1 to 5251)
  TITLE     Direct Submission
REFERENCE   13  (bases 1 to 5251)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb. Symp. Quant. Biol. 45 (Pt 1), 107-113 (1981)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Gene"; 
            date: "1982"; volume: "19"; issue: "3"; pages: "327-336"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Gene"; 
            date: "1983"; volume: "26"; issue: "1"; pages: "101-106"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "1984"; volume: "81"; issue: "13"; pages: 
            "4129-4133"
COMMENT     SGRef: number: 5; type: "Journal Article"; journalName: "Gene"; 
            date: "1985"; volume: "33"; issue: "1"; pages: "103-119"
COMMENT     SGRef: number: 6; type: "Journal Article"; journalName: "Gene 53 
            (2-3), 283-286 (1987)"
COMMENT     SGRef: number: 7; type: "Journal Article"; journalName: "Gene 53 
            (2-3), 275-281 (1987)"
COMMENT     SGRef: number: 8; type: "Journal Article"; journalName: "FEMS 
            Microbiol. Lett."; date: "1991"; volume: "65"; issue: "3"; pages: 
            "261-264"
COMMENT     SGRef: number: 9; type: "Journal Article"; journalName: "Gene"; 
            date: "1992"; volume: "111"; issue: "1"; pages: "115-118"
COMMENT     SGRef: number: 10; type: "Journal Article"; journalName: 
            "Microbiology 141 (Pt 10), 2499-2510 (1995)"
COMMENT     SGRef: number: 11; type: "Journal Article"; journalName: "Submitted 
            (20-APR-1997) Immunology, IRCM, 110 Pine Avenue West, Montreal, QC 
            H2W 1R7, Canada"
COMMENT     SGRef: number: 12; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..76
                     /label=polylinker
                     /note="polylinker"
     promoter        complement(85..103)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      128..151
                     /label=trpA transcription terminator
                     /note="trpA transcription terminator"
                     /regulatory_class="terminator"
     CDS             complement(188..979)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(1022..1069)
                     /label=synthetic promoter
                     /note="synthetic promoter"
                     /regulatory_class="promoter"
     rep_origin      complement(1261..1849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    1936..2157
                     /label=bacteriophage lambda COS site
                     /note="bacteriophage lambda COS site"
     rep_origin      2165..2593
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      2604..3251
                     /label=pJV1 replication origin
                     /note="pJV1 replication origin"
     CDS             3252..4838
                     /codon_start=1
                     /gene="pJV1 rep"
                     /product="pJV1 rep replication factor"
                     /label=pJV1 rep
                     /protein_id="AAB60866.1"
                     /translation="MLNRVSGIDACGGCGRRVLDPDTGVIYAKSSRGYVVTIGLVRCGR
                     IWFCPECSSAIRRGRTEEIKTGALRHLAAGGTLAVVVLTARHNQTTDLDSLVAALWGGP
                     LLDDKGAPVLDRSGKPRRAPGAYQRMLTAPAFYGRPEARRTRKDGTQYVRPAEDGIRHR
                     IGYIGMVRAAEVTRSKKNGYHPHLNLLVFLGGELSGTPAKGDVVGHFEPSETDLGDWED
                     WLREMWAGALKRADPKFEPSTDCDTPGCKCKGKGHGVMVSIVRSADDVALIEYLTKNQD
                     GKRERPDSVDQDLEAAGAAAMETARLDSKTGRGRKSMTPFQILYRLWDIEVAGLDPDMA
                     EGYGTPKQLRAWWAQYEEALAGRRAIEWTRGLRRHVDLDGDDDEETDLQYVYEPEAAPL
                     DGGVVLTSDAMRLVVGADAELDLDDVVRAEAYYSAVDVVTGLGGRADHVRVATAEELAE
                     VQEVLFARTQERAEESRRQRRIAEHEAEQAAAHRKRQELARCLGLLVRQRGGTQDDSAA
                     DNFVAHIHANR"
     gene            3252..4838
                     /gene="pJV1 rep"
                     /label=pJV1 rep
     terminator      4950..4993
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     regulatory      complement(4955..4994)
                     /label=rrnC transcription terminator
                     /note="rrnC transcription terminator"
                     /regulatory_class="terminator"
     promoter        5216..5234
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gcatgcacgc gtctcgaggg cccctgcagg tcgcgactct agaggatcct aagtactgcg
       61 gccgcgatcc aagcttccgg tctccctata gtgagtcgta ttaatttcga taagccacga
      121 attagcccgc ctaatgagcg ggcttttttt gaattaattc gatccccgaa ccccagagtc
      181 ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
      241 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
      301 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
      361 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
      421 tcacgacgag atcctcgccg tcgggcatac gcgccttgag cctggcgaac agttcggctg
      481 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
      541 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
      601 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
      661 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
      721 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
      781 gccgcgctgc ctcgtcctgt agttcattca gggcaccgga caggtcggtc ttgacaaaaa
      841 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
      901 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
      961 atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatcg atcagatcga
     1021 tctatgttgg tgtcattcta ccagaatcgg caaaagatgt caacctcgat aatttgattc
     1081 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1141 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1201 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1261 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1321 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1381 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1441 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1501 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1561 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1621 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1681 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     1741 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1801 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1861 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1921 catgtcgaga tccccccaaa aagcctcgct ttcagcacct gtcgtttcct ttcttttcag
     1981 agggtatttt aaataaaaac attaagttat gacgaagaag aacggaaacg ccttaaaccg
     2041 gaaaattttc ataaatagcg aaaacccgcg aggtcgccgc cccgtaacct gtcggatcac
     2101 cggaaaggac ccgtaaagtg ataatgatta tcatctacat atcacaacgt gcgtggaggg
     2161 gtcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     2221 accgctacac ttgctcgcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     2281 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     2341 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     2401 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     2461 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
     2521 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     2581 tttaacgcga attttaacaa aatcgagacg tcgccggccg cgcccgtgtg gtcgagctgg
     2641 tgactcacca ccaggagcac gccgacgtct gcgtcctgac gaccggtggg gcctctgagg
     2701 catccgcgcg gcgagccacg cgtgaggcgg gcgatgcggc aggatctacc cgaacaactc
     2761 cagaaaaagc gaaacggcca ggggtcctaa tccctgaccg cttctgacgt ccacccggct
     2821 accaaccaag ggactcgtct gtgtccagcg taagggacgc tttggcgtcc gcaaccccct
     2881 ctttgatcgg cgcgccgact cagtcgcccg cccgcgtccc cgccggcccc cgttgccgct
     2941 gcggcgccgc cctcacgatc acccccggca agcgagccaa ggagttctgc tccgaggcgt
     3001 gcaagaagcg ggccgcgcgg gcccggaagg ccgccgaccg agccgccgag gccgcccgtg
     3061 agaccgcccc tggcaaaaag ggacgcctag gtaaagggtt gacagagttt tcccaccccc
     3121 cactgaccag cgactttgcg gaagtggcag tcgtgggagg aacgggagag tccgagaggg
     3181 cggcagccct ttcggaacag accacaaggc gcgcggcgac cgctgccgta cgccgctacc
     3241 agggccgcaa ggtgctcaac cgggtctccg ggatcgacgc ctgcggcggg tgcgggcggc
     3301 gggtcctcga cccggacacc ggcgtgatct acgcgaagtc gagccgtggg tacgtcgtca
     3361 cgatcggcct ggtccgctgc gggcggatct ggttctgccc ggagtgctcc tccgcgatcc
     3421 gccgtggccg gaccgaggag atcaagaccg gtgctctgcg gcacctcgcc gccggcggca
     3481 cgctcgccgt tgtcgtcctc accgcccggc ataaccagac caccgacctc gacagcctgg
     3541 tcgccgcgct ctggggcggg cctctcctgg acgacaaggg cgccccggtc ctcgaccggt
     3601 cgggcaagcc ccgccgcgcg cccggtgcct accagcggat gctcacggcc ccggccttct
     3661 acggccgccc tgaggcccgc cgcacccgga aggacggaac gcagtacgtc cgtcccgctg
     3721 aggacggcat ccgccaccgg atcggctaca tcggcatggt ccgcgcggct gaggtcaccc
     3781 ggtccaagaa gaacggttac cacccccacc tcaacctgct ggtcttcctc ggcggcgagc
     3841 tctctggcac cccggccaag ggtgacgtcg tcggacactt tgagccctcc gagacggacc
     3901 tgggggactg ggaggactgg ctccgggaga tgtgggcggg cgccctcaag cgggctgacc
     3961 ccaagttcga gccctcgacc gactgcgaca cccccggctg caagtgcaag ggcaagggcc
     4021 acggcgtgat ggtctcgatc gtccggtcag ctgacgacgt cgcgctgatc gagtacctca
     4081 ccaagaacca ggacgggaag cgagagcggc ccgactccgt cgaccaggac ctcgaagccg
     4141 ccggcgcagc tgcgatggag accgcccgtc tggactccaa gaccggccgg ggccggaagt
     4201 ccatgacgcc gttccagatc ctctaccgac tgtgggacat cgaggtcgcc gggctcgacc
     4261 ccgacatggc cgagggctac ggcacgccga agcagctgcg cgcgtggtgg gcccagtacg
     4321 aggaagccct cgccggacga cgcgcgatcg agtggacccg aggcctgcgg cggcacgtcg
     4381 acctcgacgg tgacgacgac gaggagaccg acctccagta cgtctacgag ccggaggccg
     4441 cgccgctcga cggtggcgtc gtcctcacct ccgacgcgat gcgcctggtc gtcggagccg
     4501 acgctgaact cgacctcgac gacgtcgtcc gcgcggaggc gtactactcc gcggttgacg
     4561 tcgtcaccgg tctcggagga cgtgcggatc acgtgcgggt cgctaccgcc gaggaactcg
     4621 cggaggtgca ggaggtgctg ttcgcgcgga cgcaggaacg cgccgaggag agcaggcgcc
     4681 agcgccgaat cgcggagcac gaggccgagc aggccgccgc gcatcggaag cggcaggagc
     4741 tcgcgcggtg cctcgggctg ctcgtacggc agcgcggcgg cacgcaggac gactcggcgg
     4801 cggacaactt cgtcgcgcac atccacgcga accgctgacg acaacccccg gttgtcgccc
     4861 cgaccgtccc cggtcgacgc cgaaaacccc ggcgtgaccg aggcggcggg gcgtaactgg
     4921 atcattaacg tttacaattt tatggtgcaa attcagataa aaaaaatcct tagctttcgc
     4981 taaggatgat ttctagcgat gaccctgctg attgttcgct gaccatttcc gggtgcggga
     5041 cggcgttacc agaaactcag aaggttcgtc caaccaaacc gactctgacg gcagtttacg
     5101 agagagatga tagggtctgc ttcagtaagc cagatgctac acaattaggc ttgtacatat
     5161 tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac atacgattta
     5221 ggtgacacta tagaatacat aggatcagct t
//
