LOCUS       40924_19891        9400 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Bacteroides fragilis shuttle vector pFD1146, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Parker AC, Jeffrey Smith C.
  TITLE     Development of an IPTG inducible expression vector adapted for 
            Bacteroides fragilis
  JOURNAL   Plasmid 68 (2), 86-92 (2012)
  PUBMED    22487080
REFERENCE   2  (bases 1 to 9400)
  AUTHORS   Smith CJ.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 9400)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9400)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2012"; volume: "68"; issue: "2"; pages: "86-92"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-MAR-2012) Dept. Microbiology "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(786..1466)
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="replication protein for replication in Bacteroides"
                     /protein_id="AFI41181.1"
                     /translation="MILNRKIIFPLILFLWKIIVYFVENNISQKHIKMENKKAVKLTDF
                     QKKQENPFMKTSYRGIENHVVKKYKSNSGGDKRAVVALADTETGEVFKTSFIRQIEVDE
                     EQFTKLYLSNFAAFFDLSQAAIRVFGYFMTCMKPKNDLIIFNRKKCLEYTKYKTDKAVY
                     KGLAELVKAEIIARGPADNLWFINPLIVFNGDRVTFAKTYVSEKDFSCPKERRSRETTI
                     ITWL"
     gene            complement(786..1466)
                     /gene="repA"
                     /label=repA
     CDS             2552..3352
                     /codon_start=1
                     /gene="ermF"
                     /product="ErmF"
                     /label=ermF
                     /note="erythromycin resistance protein"
                     /protein_id="AFI41182.1"
                     /translation="MTKKKLPVRFTGQHFTIDKVLIKDAIRQANISNQDTVLDIGAGKG
                     FLTVHLLKIANNVVAIENDTALVEHLRKLFSDARNVQVVGCDFRNFAVPKFPFKVVSNI
                     PYGITSDIFKILMFESLGNFLGGSIVLQLEPTQKLFSRKLYNPYTVFYHTFFDLKLVYE
                     VGPESFLPPPTVKSALLNIKRKHLFFDFKFKAKYLAFISYLLEKPDLSVKTALKSIFRK
                     SQVRSISEKFGLNLNAQIVCLSPSQWLNCFLEMLEVVPEKFHPS"
     gene            2552..3352
                     /gene="ermF"
                     /label=ermF
     CDS             4208..4975
                     /codon_start=1
                     /gene="aad9"
                     /product="Aad9"
                     /label=aad9
                     /note="spectinomycin resistance protein"
                     /protein_id="AFI41183.1"
                     /translation="MRRIYLNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNS
                     DLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPPKQ
                     EFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFSDVR
                     RAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERI
                     LLAVRSYLGENIEWTNENVNLTINYLNNRLKKL"
     gene            4208..4975
                     /gene="aad9"
                     /label=aad9
     rep_origin      5818..6406
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            6727..6836
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             6869..7237
                     /label=traJ
                     /note="oriT-recognizing protein"
     protein_bind    7472..7493
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7508..7538
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7546..7562
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7570..7586
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7611..7631
                     /label=LacI, LacO3 binding site
                     /bound_moiety="LacI, LacO3"
     regulatory      7678..7699
                     /gene="cfxA"
                     /regulatory_class="promoter"
     gene            7678..7699
                     /gene="cfxA"
                     /label=cfxA
     protein_bind    7712..7732
                     /label=LacI, LacO1 binding site
                     /bound_moiety="LacI, LacO1"
     protein_bind    7714..7730
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             8292..9371
                     /label=lacI
                     /note="lac repressor"
ORIGIN
        1 atctttgttt tgcactctcc agccgatggg aaatgctatc tccaacccag ctttcattca
       61 aatgactaag ttcggacgaa catagtccaa ctcttttttc cctaaagttg tgaatctcgc
      121 tccccggctt cactgcttgt acatgaatac ttgttgctcc cataagttaa catttttgtg
      181 acaatcgata acagccggtg acagccggct gacagggggt taagggggct tgtcccctta
      241 cacacgcact ctttagggtg ctagtgtgct atcaccatac tgcataggtg cgaagttagt
      301 gaatgttttg taaatgcaca aataaaggga aaaacatttg gatttgcgat aataaagtac
      361 tacctttgtt gctgaccaaa cggtagctga ccgatacggg agagttacca aaatacaagc
      421 cgctggagtt aattgacgga catccgacat ctccagcggc tttatttttg cctatctgct
      481 tcgcctaggc acaccagtac ctctactaaa aatgtacttc aaagatactt attttctacc
      541 gacttgatag tttttacccc atattcttgg acatttttcc cccatgaggt tatctttgta
      601 gggtgaaaga gaaacccata aacggggata gattgaatgc tgggaagcat aaacaatcgg
      661 ggtaaggtta gcgaccttgc cttcatcccc cattataact ttacatagag gaactttatc
      721 tatccccccc cgcccaaagg ggagcgacca aacggcagct tcactcaatg gagtgttact
      781 gttcatcaaa gccaagtgat aattgtcgtt tctctgcttc ttctttcttt tgggcagcta
      841 aagtcttttt ccgaaacgta tgttttagca aatgtcactc ggtcaccatt gaatactatc
      901 agaggattaa taaaccaaag attatcggct ggtcctcggg ctatgatttc agcttttaca
      961 agttctgcaa gtcctttata aacggctttg tctgttttgt atttggtata ttctaggcat
     1021 ttttttctat tgaaaatgat taaatcattt ttgggtttca tgcaggtcat aaagtaacca
     1081 aaaacccgaa tagctgcttg tgataggtca aagaatgcag caaagttaga aagatacaat
     1141 ttagtgaatt gttcttcatc tacttctatt tgacggataa acgaagtctt aaacacttct
     1201 ccagtttcag tgtcggctaa agctactaca gctctcttat cgccaccact attactctta
     1261 tactttttaa caacatgatt ttcaatacct ctatagcttg ttttcataaa aggattttct
     1321 tgcttctttt gaaaatcggt taacttaact gcttttttat tttccatttt gatatgtttt
     1381 tgggaaatat tattctccac aaagtaaact attattttcc ataaaaacaa tattaaggga
     1441 aatattattt tcctatttag tatcatatta ggaaatcggt attttctaga ttggaaaatg
     1501 agaatttcca atatggaaaa tgccctatat tgtgtatcaa gtacttaact tattctattt
     1561 cttttattct taatataccc ccaaaacagc acaaaatcag tcacttaaaa atcatcggtc
     1621 ggggatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     1681 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     1741 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     1801 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     1861 aataatggtt tcttagtcgg gacgctcatc aatatccacc ctgcctggga taaatcctcg
     1921 ccctgcattt ttagaaccac gtttggcata cctgcgacct tgtctgcgaa gatatttgtg
     1981 cagtttgcca ccccgccgct tatcctccca aatccagcga tatatcgttt cgtgagatac
     2041 catcgcaatt ccctccaagc ggctcctgcc gacaatctgc tccgggctga atcctttctt
     2101 caacagcttt attatccgtt ttctcattgc cggtgtaagc acttccttgc gatgtttttg
     2161 ctgcttgcgc ctgtctgctt ttcgctgggc aagctccatg ctatagctac cacttcgggc
     2221 gtcgcaattg cgctttatct ccctgtaaac agtgctttta tctactccga tagcttccgc
     2281 tattgctttt ttgctcatcg gtatttgcaa catcatagaa attgcatacc tttgttcctc
     2341 ggttatatgt ttgctcatct gcaacttttt tttctttgga cggacaatta aagcaaagat
     2401 agcaaacttt atccattcag agtgagagaa agggggacat tgtctctctt tcctctctga
     2461 aaaataaatg tttttattgc ttattatccg cacccaaaaa gttgcattta taagttgaac
     2521 tcaagaagta ttcacctgta agaagttact aatgacaaaa aagaaattgc ccgttcgttt
     2581 tacgggtcag cactttacta ttgataaagt gctaataaaa gatgcaataa gacaagcaaa
     2641 tataagtaat caggatacgg ttttagatat tggggcaggc aaggggtttc ttactgttca
     2701 tttattaaaa atcgccaaca atgttgttgc tattgaaaac gacacagctt tggttgaaca
     2761 tttacgaaaa ttattttctg atgcccgaaa tgttcaagtt gtcggttgtg attttaggaa
     2821 ttttgcagtt ccgaaatttc ctttcaaagt ggtgtcaaat attccttatg gcattacttc
     2881 cgatattttc aaaatcctga tgtttgagag tcttggaaat tttctgggag gttccattgt
     2941 ccttcaatta gaacctacac aaaagttatt ttcgaggaag ctttacaatc catataccgt
     3001 tttctatcat actttttttg atttgaaact tgtctatgag gtaggtcctg aaagtttctt
     3061 gccaccgcca actgtcaaat cagccctgtt aaacattaaa agaaaacact tattttttga
     3121 ttttaagttt aaagccaaat acttagcatt tatttcctat ctgttagaga aacctgattt
     3181 atctgtaaaa acagctttaa agtcgatttt caggaaaagt caggtcaggt caatttcgga
     3241 aaaattcggt ttaaacctta atgctcaaat tgtttgtttg tctccaagtc aatggttaaa
     3301 ctgttttttg gaaatgctgg aagttgtccc tgaaaaattt catccttcgt agttcaaagt
     3361 cgggtggttg tcaagatgat ttttttggtt tggtgtagtc tttttttaag ctgccgcata
     3421 acggctggca aattggcgat ggagccgact tttagcacaa atgttgaata gaattactaa
     3481 tcttcaacat tgcacaaaag ttcaatagaa gtacttcacc ggctctattg ccaatttgtt
     3541 tgttatgtgc cgttttattc cattttatac atttaacaat tgctgaaacg taaagtcggg
     3601 tttaaacatt tcaatttcgt tttgagttga ttcttcttgt gcttctttgc catacataaa
     3661 catttgctgt tcataatttt taacagcctc ttcaatgcta ttaaattttc catcggctag
     3721 attatcagac aatatcaagg catccaccaa cccactattt actccctgcc ctgcaaaagg
     3781 cggcatcaaa tgtgcggcat ccccaatcat tgttatgggt aatggcaggt ggcacttttc
     3841 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     3901 cgctcatgag acaataaccc tgataaatgc ttcaataatc gattttcgtt cgtgaataca
     3961 tgttataata actataacta ataacgtaac gtgactggca agagatattt ttaaaacaat
     4021 gaataggttt acacttactt tagttttatg gaaatgaaag atcatatcat atataatcta
     4081 gaataaaatt aactaaaata attattatct agataaaaaa tttagaagcc aatgaaatct
     4141 ataaataaac taaattaagt ttatttaatt aacaactatg gatataaaat aggtactaat
     4201 caaaatagtg aggaggatat atttgaatac atacgaacaa attaataaag tgaaaaaaat
     4261 acttcggaaa catttaaaaa ataaccttat tggtacttac atgtttggat caggagttga
     4321 gagtggacta aaaccaaata gtgatcttga ctttttagtc gtcgtatctg aaccattgac
     4381 agatcaaagt aaagaaatac ttatacaaaa aattagacct atttcaaaaa aaataggaga
     4441 taaaagcaac ttacgatata ttgaattaac aattattatt cagcaagaaa tggtaccgtg
     4501 gaatcatcct cccaaacaag aatttattta tggagaatgg ttacaagagc tttatgaaca
     4561 aggatacatt cctcagaagg aattaaattc agatttaacc ataatgcttt accaagcaaa
     4621 acgaaaaaat aaaagaatat acggaaatta tgacttagag gaattactac ctgatattcc
     4681 attttctgat gtgagaagag ccattatgga ttcgtcagag gaattaatag ataattatca
     4741 ggatgatgaa accaactcta tattaacttt atgccgtatg attttaacta tggacacggg
     4801 taaaatcata ccaaaagata ttgcgggaaa tgcagtggct gaatcttctc cattagaaca
     4861 tagggagaga attttgttag cagttcgtag ttatcttgga gagaatattg aatggactaa
     4921 tgaaaatgta aatttaacta taaactattt aaataacaga ttaaaaaaat tataaaaaaa
     4981 ttgaaaaaat ggtggaaaca cttttttcaa tttttttgtt ttattattta atatttggga
     5041 aatattcatt ctaattggta atcagatttt agaaaacaat aaacccttgc ataactcacc
     5101 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5161 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5221 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5281 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5341 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5401 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5461 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5521 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5581 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5641 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5701 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5761 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5821 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5881 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5941 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     6001 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6061 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6121 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6181 caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag
     6241 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6301 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6361 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6421 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6481 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6541 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcttttccgc
     6601 tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat cctttttcgc
     6661 acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta tccaacggcg
     6721 tcagccgggc aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt
     6781 cccttattcg cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccggctac
     6841 cgccggcgta acagatgagg gcaagcggat ggctgatgaa accaagccaa ccaggaaggg
     6901 cagcccacct atcaaggtgt actgccttcc agacgaacga agagcgattg aggaaaaggc
     6961 ggcggcggcc ggcatgagcc tgtcggccta cctgctggcc gtcggccagg gctacaaaat
     7021 cacgggcgtc gtggactatg agcacgtccg cgagctggcc cgcatcaatg gcgacctggg
     7081 ccgcctgggc ggcctgctga aactctggct caccgacgac ccgcgcacgg cgcggttcgg
     7141 tgatgccacg atcctcgccc tgctggcgaa gatcgaagag aagcaggacg agcttggcaa
     7201 ggtcatgatg ggcgtggtcc gcccgagggc agagccatga cttttttagc cgctaaaacg
     7261 gccggggggt gcgcgtgatt gccaagcacg tccccatgcg ctccatcaag aagagcgact
     7321 tcgcggagct ggtgaagtac atcaccgacg agcaaggcaa gaccgagcgc ccaatacgca
     7381 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     7441 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     7501 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     7561 aatttcacac aggaaacagc tatgaccatg attacgccaa gcttgcatgc ggcagtgagc
     7621 gcaacgcaat tttacaaaga aaattcgaca aactgttatt tttctatcta tttatttggg
     7681 tgggaaactt tagttatgta cctttgtcgg caattgtgag cggataacaa ttgtcgactc
     7741 tagaggatcc ccgggtaccg agctcgaatt cccaaaaggt ctaaaagtaa attttatcct
     7801 ttctgcaagt atctatagga tggcaactgc atttttttct ttttgggcag cccttattaa
     7861 aatttattct tattttaggt tatatacatt catgtccatt tatgtaaaaa attcctgctg
     7921 accttgttta tgtcttgtca gtcaccattt gcaaaaccat atttgaccct caaagaggct
     7981 gaatttgata agtaacttgc tacatactca taataaggag ctaaatagaa cacgaatggg
     8041 aaatacacaa atgctaaact aaagaagata ttggccaaaa taaacgctat accgagagag
     8101 aaacttgatt tttcaacttc ctaaaacggt gttgttcaaa catttctact tatttgtact
     8161 taccagttga acctacgttt ccctaataaa atgtctatgg taaaaagtta aaaaatcctc
     8221 ctacttttgt tagatatatt tttttgtgta attttgtaat cgttatgcgg gtcgacaaat
     8281 aagaaacaat tatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt
     8341 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa
     8401 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg
     8461 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt
     8521 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
     8581 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
     8641 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
     8701 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca
     8761 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat
     8821 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
     8881 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
     8941 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca
     9001 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca
     9061 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg
     9121 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg
     9181 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     9241 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg
     9301 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     9361 aaagcgggca gtgagcgcaa cgcaattaat gtgagttccc
//
