LOCUS       V014096                 6761 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014096
VERSION     V014096
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(343..1218)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        1245..1529
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     CDS             1607..2320
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     oriT            2799..2886
                     /label="RSF1010 oriT"
                     /note="origin of transfer of the broad-host-range plasmid
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      complement(4370..4958)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5132..5989)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5990..6094)
                     /label="AmpR promoter"
     CDS             6423..6536
                     /gene="rpmJ"
                     /label="Large ribosomal subunit protein bL36A"
                     /note="Large ribosomal subunit protein bL36A from
                     Escherichia coli (strain K12). Accession#: P0A7Q6"
ORIGIN
        1 gcaagacccg ttctaaagcc atcctggctg cagcgggtat cgctgaaaat gttaagatcc
       61 tgggaggaga tatacatgcg gccgcgaaag ctttaatgcg gtagtttatc acagttaaat
      121 tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc
      181 accgtcaccc tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg
      241 cgggatcgat gcataatgtg cctgtcaaat ggacgaagca gggattctgc aaaccctatg
      301 ctactccgtc aagccgtcaa ttgtctgatt cgttaccaat tatgacaact tgacggctac
      361 atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc cggtgcattt
      421 tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac cgacggtggc
      481 gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg
      541 ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac gcgacggcga
      601 caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct
      661 gatgtactga caagcctcgc gtacccgatt atccatcggt ggatggagcg actcgttaat
      721 cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca gctccgaata
      781 gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga aatgcggctg
      841 gtgcgcttca tccgggcgaa agaaccccgt attggcaaat attgacggcc agttaagcca
      901 ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt cgcgagcctc
      961 cggatgacga ccgtagtgat gaatctctcc tggcgggaac agcaaaatat cacccggtcg
     1021 gcaaacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg tgagattgag
     1081 aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac cgttggcctc
     1141 aatcggcgtt aaacccgcca ccagatgggc attaaacgag tatcccggca gcaggggatc
     1201 attttgcgct tcagccatac ttttcatact cccgccattc agagaagaaa ccaattgtcc
     1261 atattgcatc agacattgcc gtcactgcgt cttttactgg ctcttctcgc taaccaaacc
     1321 ggtaaccccg cttattaaaa gcattctgta acaaagcggg accaaagcca tgacaaaaac
     1381 gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt
     1441 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt
     1501 tttatcgcaa ctctctactg tttctccata cccgtttttt tgggctagca ggaggaattc
     1561 accatggtac ccggggatcc tctagattta agaaggagat atacatatga gtaaaggaga
     1621 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
     1681 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
     1741 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
     1801 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
     1861 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
     1921 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
     1981 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
     2041 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
     2101 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
     2161 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
     2221 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
     2281 agctgctggg attacacatg gcatggatga actatacaaa taaatgtcca gacctgcagg
     2341 catgcaagct atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct
     2401 tttagccgct ttagcggcct ttccccctac ccgaagggtg ggggcgcgtg tgcagccccg
     2461 cagggcctgt ctcggtcgat cattcagccc ggctcatcct tctggcgtgg cggcagaccg
     2521 aacaaggcgc ggtcgtggtc gcgttcaagg tacgcatcca ttgccgccat gagccgatcc
     2581 tccggccact cgctgctgtt caccttggcc aaaatcatgg cccccaccag caccttgcgc
     2641 cttgtttcgt tcttgcgctc ttgctgctgt tcccttgccc gcacccgctg aatttcggca
     2701 ttgattcgcg ctcgttgttc ttcgagcttg gccagccgat ccgccgcctt gttgctcccc
     2761 ttaaccatct tgacacccca ttgttaatgt gctgtctcgt aggctatcat ggaggcacag
     2821 cggcggcaat cccgacccta ctttgtaggg gagggcgcac ttaccggttt ctcttcgaga
     2881 aactggccta acggccaccc ttcgggcggt gcgctctccg agggccattg catggagccg
     2941 aaaagcaaaa gcaacagcga ggcagcatgg cgatttatca ccttacggcg aaaaccggca
     3001 gcaggtcggg cggccaatcg gccagggcca aggccgacta catccagcgc gaaggcaagt
     3061 atgcccgcga catggatgaa gtcttgcacg ccgaatccgg gcacatgccg gagttcgtcg
     3121 agcggcccgc cgactactgg gatgctgccg acctgtatga acgcgccaat gggcggctgt
     3181 tcaaggaggt cgaatttgcc ctgccggtcg agctgaccct cgaccagcag aaggcgctgg
     3241 cgtccgagtt cgcccagcac ctgaccggtg ccgagcgcct gccgtatacg ctggccatcc
     3301 atgccggtgg cggcgagaac ccgcactgcc acctgatgat ctccgagcgg atcaatgacg
     3361 gcatcgagcg gcccgccgct cagtggttca agcggtacaa cggcaagacc ccggagaagg
     3421 gcggggcaca gaagaccgaa gcgctcaagc ccaaggcatg gcttgagcag acccgcgagg
     3481 catgggccga ccatgccaac cgggcattag agcgggctgg ccacgacgcc cgcattgacc
     3541 acagaacact tgaggcgcag ggcatcgagc gcctgcccgg tgttcacctg gggccgaacg
     3601 tggtggagat ggaaggccgg ggcatccgca ccgaccgggc agacgtggcc ctgaacatcg
     3661 acaccgccaa cgcccagatc atcgacttac aggaataccg ggaggcaata gaccatgaac
     3721 gcaatcgaca gagtgaagaa atccagaggc atcaacgagt tagcggagca gatcgaaccg
     3781 ctggcccaga gcatggcgac actggccgac gaagcccggc aggtcatgag ccagacccag
     3841 caggccagcg aggcgcaggc ggcggagtgg ctgaaagccc agcgccagac aggggcggca
     3901 tgggtggagc tggccaaaga gttgcgggag gtagccgccg aggtgagcag cgccgcgcag
     3961 agcgcccgga gcgcgtcgcg ggggtggcac tggaagctat ggctaaccgt gatgctggct
     4021 tccatgatgc ctacggtggt gctgctgatc gcatcgttgc tcttgctcga cctgacgcca
     4081 ctgacaaccg aggacggctc gatctggctg cgcttggtgg cccgatgaag aacgacagga
     4141 ctttgcaggc cataggccga cagctcaagg ccatgggctg tgagcgctct tccgcttcct
     4201 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4261 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4321 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4381 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4441 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4501 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4561 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4621 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4681 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4741 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4801 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4861 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4921 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4981 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     5041 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     5101 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     5161 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     5221 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     5281 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     5341 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     5401 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
     5461 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     5521 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     5581 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     5641 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     5701 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
     5761 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5821 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     5881 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     5941 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     6001 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     6061 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     6121 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     6181 cctttcgtct tcaagaattc gagctcggta cccggggatc ccggagttca tgcgtgatgc
     6241 aatgaaagtg ccgttctact tcggtgggac ctcactgctt atcgttgttg tcgtgattat
     6301 ggactttatg gctcaagtgc aaactctgat gatgtccagt cagtatgagt ctgcattgaa
     6361 gaaggcgaac ctgaaaggct acggccgtta attggtcgcc tgagaagtta cggagagtaa
     6421 aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa ctgcaaaatc gttaagcgtg
     6481 atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca taaacagcgc caaggctgat
     6541 tttttcgcat atttttcttg caaagttggg ttgagctggc tagattagcc agccaatctt
     6601 ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg ggcttttcag catggtacgt
     6661 acatattaaa tagtaggagt gcatagtggc ccgtatagca ggcattaaca ttcctgatca
     6721 gaaacacgcc gtgatcgcgt taacttcgat ctacggtgtc g
//
