LOCUS       V006289                 5044 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006289
VERSION     V006289
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Hartnett J, Slater M.
  TITLE     pFC8A (HaloTag) CMV Flexi Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Hartnett J, Slater M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2005) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 5044)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5044)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-JUL-2005) Scientific Communications, Promega Corporation, 2800
            Woods Hollow Rd., Madison, WI 53711, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     promoter        1033..1051
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1087..1419
                     /label="barnase"
                     /note="ribonuclease from Bacillus amyloliquefaciens"
     CDS             1456..1467
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             1501..2376
                     /gene="dhaA"
                     /label="Haloalkane dehalogenase"
                     /note="Haloalkane dehalogenase from Rhodococcus sp.
                     Accession#: P0A3G3"
     polyA_signal    complement(2513..2634)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        2881..2985
                     /label="AmpR promoter"
     CDS             2986..3843
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      complement(4004..4592)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    4708..4991
                     /label="cer region"
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggaa taagtaagga
     1081 atccacatgg cacaggttat caacacgttt gacggggttg cggattatct tcagacatat
     1141 cataagctac ctgataatta cattacaaaa tcagaagcac aagccctcgg ctgggtggca
     1201 tcaaaaggga accttgcaga cgtcgctccg gggaaaagca tcggcggaga catcttctca
     1261 aacagggaag gcaaactccc gggcaaaagc ggacgaacat ggcgtgaagc ggatattaac
     1321 tatacatcag gcttcagaaa ttcagaccgg attctttact caagcgactg gctgatttac
     1381 aaaacaacgg accattatca gacctttaca aaaatcagat aatgtttaat gaccccgtgt
     1441 cgagctctcg atctgatcga aggtcgtggt atccctcgta actctcgtgt tgatgcggga
     1501 tccgaaatcg gtacaggctt ccccttcgac ccccattatg tggaagtcct gggcgagcgt
     1561 atgcactacg tcgatgttgg accgcgggat ggcacgcctg tgctgttcct gcacggtaac
     1621 ccgacctcgt cctacctgtg gcgcaacatc atcccgcatg tagcaccgag tcatcggtgc
     1681 attgctccag acctgatcgg gatgggaaaa tcggacaaac cagacctcga ttatttcttc
     1741 gacgaccacg tccgctacct cgatgccttc atcgaagcct tgggtttgga agaggtcgtc
     1801 ctggtcatcc acgactgggg ctcagctctc ggattccact gggccaagcg caatccggaa
     1861 cgggtcaaag gtattgcatg tatggaattc atccggccta tcccgacgtg ggacgaatgg
     1921 ccagaattcg cccgtgagac cttccaggcc ttccggaccg ccgacgtcgg ccgagagttg
     1981 atcatcgatc agaacgcttt catcgagggt gcgctcccga tgggggtcgt ccgtccgctt
     2041 acggaggtcg agatggacca ctatcgcgag cccttcctca agcctgttga ccgagagcca
     2101 ctgtggcgat tccccaacga gctgcccatc gccggtgagc ccgcgaacat cgtcgcgctc
     2161 gtcgaggcat acatgaactg gctgcaccag tcacctgtcc cgaagttgtt gttctggggc
     2221 acacccggcg tactgatccc cccggccgaa gccgcgagac ttgccgaaag cctccccaac
     2281 tgcaagacag tggacatcgg cccgggattg ttcttgctcc aggaagacaa cccggacctt
     2341 atcggcagtg agatcgcgcg ctggctcccg gcactctaat ctagagtcga cctgcaggca
     2401 tgcaagctga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
     2461 ctgagcaata actagcataa ccccttgggg cggccgcttc gagcagacat gataagatac
     2521 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     2581 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     2641 aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt ttttttaagc
     2701 aagtaaaacc tctacaaatg tggtaaaatc gaattctaat ggatcctctt tgcgcttgcg
     2761 ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc
     2821 ggactggctt tctacgtaat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     2881 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     2941 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     3001 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     3061 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     3121 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     3181 atgatgagca ctttcaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     3241 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     3301 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     3361 accatgagtg ataacactgc ggccaactta cttctgacaa ctatcggagg accgaaggag
     3421 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     3481 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     3541 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     3601 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     3661 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     3721 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     3781 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     3841 tggtaattcg aaatgaccga ccaagcgacg cccaaccggt atcagctcac tcaaaggcgg
     3901 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3961 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4021 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4081 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4141 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4201 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4261 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4321 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4381 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4441 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4501 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4561 agcagattac gcgcagaaaa aaaggatttc aagaagatcc tttgatcttt tctacggggt
     4621 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4681 ggatcttcac ctagatcctt ttatagtccg gaaatacagg aacgcacgct ggatggccct
     4741 tcgctgggat ggtgaaacca tgaaaaatgg cagcttcagt ggattaagtg ggggtaatgt
     4801 ggcctgtacc ctctggttgc ataggtattc atacggttaa aatttatcag gcgcgattgc
     4861 ggcagttttt cgggtggttt gttgccattt ttacctgtct gctgccgtga tcgcgctgaa
     4921 cgcgttttag cggtgcgtac aattaaggga ttatggtaaa tccacttact gtctgccctc
     4981 gtagccatcg agataaaccg cagtactccg gccacgatgc gtccggcgta gaggatcgag
     5041 atct
//
