LOCUS       40924_19771        9609 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFB-Neo-LacZ, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Jaskula J, Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2001) Technical Services, Stratagene, 11011 N 
            Torrey Pines Rd, La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 9609)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9609)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (29-JUN-2001) Technical Services, Stratagene, 11011 N Torrey Pines 
            Rd, La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             289..758
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    823..1180
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1245..1661
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1671..2044
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     misc_feature    5167..5629
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             5634..5663
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             5768..5785
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             5806..6579
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     LTR             6642..7235
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    7446..7586
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7772..8360)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8534..9391)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9392..9496)
                     /label=AmpR promoter
ORIGIN
        1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
       61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
      121 ccctattccc cacactcacc ggagccaaag ccgcgggaca tatacatgtg aaagacccca
      181 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat
      241 aactgagaat agaaaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc
      301 caaagcggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa
      361 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
      421 caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga
      481 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc
      541 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
      601 aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc
      661 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
      721 ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg
      781 ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta
      841 tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta
      901 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc
      961 cggccgcaac cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg
     1021 agtccaaaaa tcccgatcgt tttggactct ttggtgcacc ccccttagag gagggatatg
     1081 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
     1141 cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc
     1201 tctgtctgac tgtgtttctg tatttgtctg aaaatatggg cccgggccag actgttacca
     1261 ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt
     1321 cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta
     1381 acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca
     1441 aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg
     1501 aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc
     1561 ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc
     1621 gatcctccct ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga
     1681 tcttatatgg ggcacccccg ccccttgtaa acttccctga ccctgacatg acaagagtta
     1741 ctaacagccc ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct
     1801 ggagacctct ggcggcacgt accaagaaca actggaccga ccggtggtac ctcaccctta
     1861 ccgagtcggc gacacagtgt gggtccgccg acaccagact aagaacctag aacctcgctg
     1921 gaaaggacct tacacagtcc tgctgaccac ccccaccgcc ctcaaagtag acggcatcgc
     1981 agcttggata cacgccgccc acgtgaaggc tgccgacccc gggggtggac catcctctag
     2041 actgccggat cgaattcgtt aactcgagac catggtcgtt ttacaacgtc gtgactggga
     2101 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     2161 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     2221 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga
     2281 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc
     2341 gcccatctac accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga
     2401 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg
     2461 ccagacgcga attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg
     2521 ctgggtcggt tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt
     2581 acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct
     2641 ggaagatcag gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa
     2701 accgactaca caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg
     2761 cgctgtactg gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac
     2821 agtttcttta tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga
     2881 aattatcgat gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa
     2941 cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac
     3001 cgccgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat
     3061 tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca
     3121 cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct
     3181 gctgatgaag cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct
     3241 gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac
     3301 ccacggcatg gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag
     3361 cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc
     3421 gctggggaat gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc
     3481 tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga
     3541 tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa
     3601 atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga
     3661 atacgcccac gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg
     3721 tcagtatccc cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa
     3781 atatgatgaa aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa
     3841 cgatcgccag ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct
     3901 gacggaagca aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga
     3961 agtgaccagc gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc
     4021 gctggatggt aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa
     4081 acagttgatt gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac
     4141 agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa accgggcaca tcagcgcctg
     4201 gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat
     4261 ccccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat aagcgttggc
     4321 aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa aaacaactgc
     4381 tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt ggcgtaagtg
     4441 aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg ggccattacc
     4501 aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg gcgctgatta
     4561 cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg aaaacctacc
     4621 ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg agcgatacac
     4681 cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag cgggtaaact
     4741 ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc tgttttgacc
     4801 gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc gaaaacggtc
     4861 tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc gacttccagt
     4921 tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc catctgctgc
     4981 acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt ggtggcgacg
     5041 actcctggag cccgtcagta tcggcggaat tccagctgag cgccggtcgc taccattacc
     5101 agttggtctg gtgtcaaaaa taaggatccg gttattttcc accatattgc cgtcttttgg
     5161 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     5221 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     5281 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     5341 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     5401 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     5461 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     5521 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa acgtctaggc
     5581 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatcgataat atggaacaaa
     5641 aacttatttc tgaagaagac ttggacacca aactttcctg ccgctcgatt tctccaccca
     5701 ggtgaactcc tccctcacct ccccgacggg gcgaggctcc atggctgccc cctgcctgca
     5761 cccgtccctg gtgccacgcg gttccatggg atcgtttcgc atgaattgca cgcaggttct
     5821 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     5881 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     5941 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     6001 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     6061 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     6121 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     6181 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     6241 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     6301 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     6361 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     6421 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     6481 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     6541 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     6601 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt
     6661 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg
     6721 agaatagaga agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac
     6781 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct
     6841 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     6901 acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt
     6961 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
     7021 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
     7081 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
     7141 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
     7201 agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga
     7261 cccctgccca gggaccaccg acccaccacc gggaggtaag ctggctgcct cgcgcgtttc
     7321 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     7381 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     7441 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     7501 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     7561 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     7621 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7681 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7741 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7801 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7861 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7921 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7981 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8041 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8101 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8161 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     8221 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8281 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8341 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8401 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8461 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8521 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8581 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8641 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8701 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8761 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8821 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
     8881 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8941 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9001 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9061 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9121 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
     9181 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9241 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9301 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9361 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9421 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9481 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     9541 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaattga
     9601 acaagatgg
//
