LOCUS       V012966                 9241 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V012966
VERSION     V012966
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     CDS             3259..3975
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    3978..4043
                     /label="MCS"
                     /note="multiple cloning site"
     promoter        4076..4575
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             4596..5192
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    5209..5797
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6004..6637
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6766..6782)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6790..6806)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6814..6844)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6859..6880)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7168..7756)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7930..8787)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8788..8892)
                     /label="AmpR promoter"
     polyA_signal    8940..9074
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttcct cagcctcttg gtgtaatctg
     2221 gatatcagat gcaatcagcg aacagcctga gttttggaat tagatctgag ttcaatccca
     2281 tatctcccat ttactagctg tgtgatcttg gctgaattac tgaagctctc tgaacctcat
     2341 ttcgccatct gtaaaatggg gatgtggtta tcttccccac tacatggctc tcgtgagaat
     2401 ccgcggagat cacatttgta aacacttctc tcgctatgcc tggcactttg ttggggccca
     2461 ataaaataaa aatccatgga ctctcagagg ctccggtact caaatgagcc tcctggatcc
     2521 acgtctcttt gattagccat ctgcaagctg gcatttctga gcgagggact tttcaaaaca
     2581 ggctgctcaa gtttcttggc caataatagg cgccgggttc tgtgcggtgg gaacgagtac
     2641 caccaacccc agcaggagac caagcagaaa tcaccatggg agtgcaagct aagaaagggc
     2701 aaaagaagaa agagaagggc agaaaaacaa aacaaaatga aaccactcag gcagcgactt
     2761 acagtcttaa agagagaatt cccggaaggg agacaaggca gtttcttttt ctgtgtgaca
     2821 ataaaaaacg gtaaacaagc ctccagaagc tcattcagcc cccatataac tttttcgaga
     2881 aagaaaaggt gccgttcttc cgagccctcc ggcttaacca ctgcttcggt gctgacttat
     2941 ttcctacgtc tgagaactgc cagaaaatga gcaatatctg ttctgaaggc tgcaggctct
     3001 ctccccgccc ccgcggggcg gcgcgcactc acccacccgc gccggagcgg acctttggct
     3061 tggcttgtca gggcttgtcc aggagttccg ctcctctctc caaccggggt ccccctccag
     3121 cgaccctaaa gcttcccaga cttccgcttc aattcctgtc cgtttagtga accgtcagat
     3181 cgcctggaga cgccatccac gctgttttga cctccataga agacaccgac tctactagag
     3241 gatctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     3301 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     3361 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     3421 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     3481 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     3541 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     3601 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     3661 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     3721 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     3781 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     3841 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     3901 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     3961 gacgagctgt acaagtatcc ggactcagat ctcgagctca agcttcgaat tctgcagtcg
     4021 acggtaccgc gggcccggga tccaccggat ctagataact gatcataatt ctaccgggta
     4081 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     4141 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     4201 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     4261 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     4321 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     4381 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     4441 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     4501 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     4561 tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag cccacggtgc
     4621 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     4681 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     4741 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     4801 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     4861 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     4921 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     4981 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     5041 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     5101 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     5161 gcacctggtg catgacccgc aagcccggtg cctgaccgcg tctggaacaa tcaacctctg
     5221 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     5281 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     5341 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     5401 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     5461 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     5521 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     5581 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     5641 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     5701 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     5761 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag
     5821 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     5881 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     5941 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg
     6001 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     6061 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     6121 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     6181 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     6241 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     6301 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     6361 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     6421 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     6481 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6541 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6601 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     6661 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc
     6721 tagcgtttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     6781 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6841 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6901 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6961 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7021 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7081 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7141 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7201 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7261 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7321 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7381 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7441 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7501 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7561 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7621 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7681 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7741 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7801 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7861 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7921 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7981 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8041 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8101 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8161 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8221 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8281 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8341 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8401 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8461 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8521 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8581 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8641 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8701 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8761 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8821 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8881 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca
     8941 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     9001 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     9061 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
     9121 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
     9181 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
     9241 t
//
