LOCUS       V006299                 8702 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006299
VERSION     V006299
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8702)
  AUTHORS   Cote P, Sulea T, Dignard D, Wu C, Whiteway M.
  TITLE     Evolutionary reshaping of fungal mating pathway scaffold proteins
  JOURNAL   MBio 2 (1), e00230-10 (2011)
   PUBMED   21249169
REFERENCE   2  (bases 1 to 8702)
  AUTHORS   Cote P.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount
            Av., Montreal, QC H4P2R2, Canada
REFERENCE   3  (bases 1 to 8702)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8702)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio";
            date: "2011"; volume: "2"; issue: "1"; pages: "e00230-10"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount Av.,
            Montreal, QC H4P2R2, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        626..644
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1124..1933
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     primer_bind     2060..2076
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(2189..2917)
                     /note="RPS1 locus; cloning site"
     CDS             complement(3086..5392)
                     /codon_start=1
                     /product="FAR1"
                     /label="FAR1"
                     /note="Candida albicans FAR1"
                     /protein_id="ADW40667.1"
                     /translation="MRKLFHSPKKRTSTSTKLESILPFPAETKKYNNIKCSLCQELLSL
                     TLPGELIVLLECGHMSHKNCLSLIQSVPVCSECNVMSNYDYPPFTPVDQIQSPAKLNTQ
                     LETPSFTSSDQTIYNPRVTCTSEISEVTLTPPYEIAYVLNVQAPLIFNAHKPTLEDLQL
                     KERVEEFLKVHLDTDKDVGQLVIFDILEVSVTGKSWDLALVCLFENYFLIYENELLVGI
                     ISVQHDISSVDVDEELILNLAKDSLPELRLRHSNKLVVQKWGTLLLEIIDNESVVTNIY
                     QLTNTYWTHLPPEICVPQNLTKLNNAILSGTTISNSDIARILPTPKQLLLNLILALPLV
                     NQTSLTDLEYKTQIQQIFQKTLHELRPNDKLGVIFLGIDGSKKPCAKGSFVGCTEPSWS
                     EWKQVIRDITIVPNSFQSNLQEINVAIEKCMELYPFIPRTTSSVNKFLILSPNTYSQLP
                     KDAPKSKFIDAINEKLSCTIVCVGSTYGNYQPFKSITTFGTPFLRFESFAKFGSLLVYY
                     INENLHKICIPKLTLTLKASQGFRFTDLDSRGAIDGSQLTLTIKDIVPQTQKLIVLKVQ
                     ANPQFVASKYQTLPIFEYDGYWFYEKYLDTKLISTRVKIGVSQQPPTPIDAEARGSFTE
                     YYLDIPLLPPVSPARDAVFAKRQTELTIINSLKKSIALGESQVSLDIIKSCISLAHGLI
                     RTSLEGSTKSHTNESSPECEYSMLQLLMAVRYTDKGNVEYIDFLVNHMKSIAKLLESDL
                     DAGKLRCSDLMNSLI"
     regulatory      complement(5405..6428)
                     /label="ACT1"
                     /note="ACT1"
                     /regulatory_class="promoter"
     promoter        complement(6461..6479)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6500..6516)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6524..6540)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6548..6578)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6593..6614)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6902..7490)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(8209..8587)
                     /label="ISS"
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of
                     5'-AACGTT-3' (Sato et al., 1996)"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgcggcc gacttggcca agcctagatc ccgactaata ggaattgatt
      721 tggatggtat aaacggaaac aaaaaaaaga gctggtacta ctttctttaa aattatttta
      781 ttatttgatt ttatttaata gtatatatta tattttgaac gtagattatt ttgttgaaag
      841 ttgctgtagt gccattgatt cgtaacacta attctgtatt agtcattcct cttgtttgat
      901 agtatccaaa aaaacggcta tttttttgca atcttatttc ctgcatatta tacagataac
      961 ataatgaaag aaaaaatctt tttttttgtt cttcaatgat gatttcaacc attcttttaa
     1021 acattgatca attcctgagc aacaacccca tacacactgg tttatatacc gcccctttta
     1081 cagttgaaga aagaaataga aatagaaata gcaaacaaaa gatatgacag tcaacactaa
     1141 gacctatagt gagagagcag aaactcatgc ctcaccagta gcacagcgat tatttcgatt
     1201 aatggaactg aagaaaacca atttatgtgc atcaattgac gttgatacca ctaaggaatt
     1261 ccttgaatta attgataaat taggtcctta tgtatgctta atcaagactc atattgatat
     1321 aatcaatgat ttttcctatg aatccactat tgaaccatta ttagaacttt cacgtaaaca
     1381 tcaatttatg atttttgaag atagaaaatt tgctgatatt ggtaataccg taaagaaaca
     1441 atatattggt ggagtttata aaattagtag ttgggcagat attaccaatg ctcatggtgt
     1501 cactgggaat ggagtggttg aaggattaaa acagggagct aaagaaacca ccaccaacca
     1561 agagccaaga gggttattga tgttagctga attatcatca gtgggatcat tagcatatgg
     1621 agaatattct caaaaaactg ttgaaattgc taaatccgat aaggaatttg ttattggatt
     1681 tattgcccaa cgtgatatgg gtggccaaga agaaggattt gattggctta ttatgacacc
     1741 tggagttgga ttagatgata aaggtgatgg attaggacaa caatatagaa ctgttgatga
     1801 agttgttagc actggaactg atattatcat tgttggtaga ggattgtttg gtaaaggaag
     1861 agatccagat attgaaggta aaaggtatag aaatgctggt tggaatgctt atttgaaaaa
     1921 gactggccaa ttataaatgt gaagggggag attttcactt tattagattt gtatatatgt
     1981 agaataaata aataaataag ttaaataaat aattaaataa gggtggtaat tattactatt
     2041 tacaatcaaa ggtggtcctt ctagaactag tggatccccc agatcattat ccttaaacag
     2101 attctaaaac aacatctttg aaaccagaag aaactttctt acctttttct tcggttgaac
     2161 cttcaccgtg caaagccaat aatgaacccc agtcgaattt tggttgtttc aacaatttga
     2221 cttttctgat gtggacattt tgtaatggga aaatggtttg gggggatttt tcaatttcac
     2281 ggccaatgac ttctggaatc aatttggaag ttaattgagc taaagtcagt tggaaacttc
     2341 tctttgcata atttcaatca tcttctttct gacttctctc aatttagagg attgagcgta
     2401 agtagtttcc ttgatttggt ttggttgtct tttggtgaaa gcaatggcaa aaactctcaa
     2461 aacgtaatcg tcggaagttt taacagtgac attagcttca actaatgatt gccattttct
     2521 gaccaatgat cttaatttgt cagaagtgaa atccaaacca tgaggcctcc atgaggcctc
     2581 catgaggcct ccatgaggcc tccatggagg cctcatggaa gttggtcaac aagtttttac
     2641 cttggacttc atcaactctc aatttgattt ttctgtaaga gtggtcttcg gaaccttgta
     2701 agtcggccaa acaaacttcg aaaactctac ctttcaagcc atcagcggca ttctttaaac
     2761 cggtagatct gttgatcaaa gttttaccaa catttctgtt ttcaaaagtg gttggagctt
     2821 tgatgtcaaa ccaatctttt ctggtgaatg ggtcaacgac cttctttttt aatcctttct
     2881 ttcctttgga caatctcttg tttttaccga cagccatgtt gtacttgagt tggatctacg
     2941 cgaaatttga aatataaata acgttcttaa tactaacata actataaaaa aataaatagg
     3001 gacgctagca tatgtcgacg cgtttcgctt ttatcatgtc tgcacgacac gatgatggtt
     3061 gcaccagcac aaaaaaaaaa catttctata ttaaactatt catcaaatcg gaacatcgta
     3121 atttacctgc atcgaggtcc gattctagta gcttggcaat acttttcata tgatttacca
     3181 agaaatcaat atactcaaca ttgcctttgt cagtgtatct tactgccatt agcaactgta
     3241 acatactata ctcacactca gggctagatt cattggtgtg tgattttgta gagccttcaa
     3301 gagaagtacg tatcaatccg tgtgctaacg atatgcagct tttaattata tccagtgaca
     3361 cttggctttc acccagcgcg atgcttttct ttaatgagtt aattatggtt agctcagttt
     3421 gtcttttggc aaatacagca tctcttgctg gcgacacggg aggtagtaat gggatgtcta
     3481 aatagtattc cgtaaagctt ccccttgctt ccgcatcaat aggtgtcggt ggttgctggc
     3541 tgacacctat cttgactctc gtacttatca acttggtgtc gaggtatttc tcataaaacc
     3601 aatacccatc atattcaaat attggaagcg tctgatattt ggaagcaaca aattgggggt
     3661 ttgcttgcac tttgagcaca atcagttttt gcgtctgtgg gacaatatct ttaatagtaa
     3721 gtgtcaactg acttccatca atagcccctc tggaatcaag atcagtgaac ctaaaaccct
     3781 gagatgcctt taatgttaat gtcaattttg gaatacatat tttgtggagg ttttcgttga
     3841 tatagtatac tagtaagctg ccaaacttcg cgaaagactc aaatcgtagg aaaggagtgc
     3901 caaatgtagt aatagacttg aatggttggt agttgccata tgtgctgccg acacaaacaa
     3961 ttgtgcaaga tagtttttcg tttattgcgt caataaattt ggatttgggt gcgtcttttg
     4021 gcagctgtga gtatgtgttt gggctgagta ttagaaactt gttaactgat gatgttgtac
     4081 gtggaataaa agggtatagc tccatacact tttctatagc aacatttatt tcctgtaagt
     4141 tactttgaaa tgagtttgga actattgtaa tgtctcgtat tacctgcttc cactcactcc
     4201 agcttggttc agtgcaccca acaaaggacc ctttggcaca cggttttttt gacccgtcta
     4261 ttccaagaaa aattactccc aacttgtcat ttggtctgag ttcatgtaaa gttttttgaa
     4321 agatttgttg gatctgtgtt ttgtattcta gatcggttaa agatgtttgg ttaactagtg
     4381 gtagagcaag aattaaattt aacagtagct gtttaggtgt tggtagtatt cttgcgatat
     4441 cggaatttga aatggttgtt ccggataaga ttgcattgtt tagtttggtt aaattttgtg
     4501 gtacacaaat ctctgggggc aagtgggtcc aatatgtatt agtcaattgg taaatgttag
     4561 tgacgacact ctcattgtct attatttcaa gtagtagagt accccatttt tggaccacaa
     4621 gcttgtttga atgcctgagt cgcaattctg gaagtgaatc ttttgctaga tttagaatga
     4681 gttcctcatc cacgtcaaca ctgcttatat cgtgctgaac agatattatt cccaccaaga
     4741 gctcgttttc ataaattaaa aagtagtttt caaatagaca caccaatgcc aggtcccagc
     4801 tcttgcccgt aacactaact tcaagtatat caaaaataac aagttgtcct acatccttat
     4861 cggtgtcaag atgcaccttc aaaaactctt cgaccctttc ttttagctgt aaatcttcta
     4921 gcgtgggttt atgagcgtta aaaataagtg gagcctgaac attcaacaca tacgctattt
     4981 catatggtgg ggttaaagtt acttcggaaa tctcactggt acatgttact cttggattgt
     5041 agatggtttg gtcgctcgag gtaaaggatg gtgtttccaa ttgagtgttt aatttggcag
     5101 gcgactgaat ctggtccacg ggtgtgaatg gagggtagtc gtaattcgac atcacattac
     5161 attcactaca aacaggcact gattgtatta aagacaagca gtttttatgg gacatgtggc
     5221 cacactctaa caggacaata agctctccgg gcaacgtcaa gcttagtagt tcttgacaca
     5281 acgaacattt aatgttatta tactttttag tctctgctgg gaaaggtagt atagattcaa
     5341 gctttgttga ggtggaagtt ctctttttag gagagtggaa cagtttgcgc atcgacgcgt
     5401 atataagctt tttgaatgat tatatttttt taatattaat atcgagataa tgtttctaag
     5461 aaagaaagaa aaccaggagt gaaaattaga aaaggaaagg aaaggaaaaa aagaaaaatc
     5521 tgaaaatata taaaaaaaaa ttgtttcgtt ggcaataaat cctggtgaga acagcgaccg
     5581 aaagcaaata agaacaaaat ataagtgtat tacgttgaac aactaattaa cgtgtgtgta
     5641 tggatctttt tttctttttt ctctttaacc gactataaac aacaaacatt tttgggcagt
     5701 gcacacacac tacttaatat acacagcata aattacacga ttagaaaaca aattagctta
     5761 ttaaaataac ctaatcaaac cgaatatttt atggtattac gagtaaacta tataatataa
     5821 atagcacaca cccacaacaa caacaaagga aaactaaaaa ttttttcttt ttgaaagggc
     5881 cgttttcttt attattctct agttttgacg ctgaaatatt ccttgtactg ttgcaaattt
     5941 gtatttggtc tgccttatct tcttttctac actttttact tatgagctct ataattcaca
     6001 gataagtttc aggtttgcca gatatacttg cttcattggt tactaaactc tctgtaaaca
     6061 attgacttgc ttatgtaaga ggctactttt ctagttttgc agtcattcat ttaaaaacaa
     6121 ttgatcctga aggctctaaa gacatagtag gtttaccttg agtgtatatt tccgtggcat
     6181 gtttaaaggc cccttggcca taggatattg gtggcggcac agtaagcatt aaaattgttg
     6241 tttcgcggaa ttacaacatt tcttctttta cataataatg tcactccttt ttggttggca
     6301 tagccgagat ctttttttaa cacagacttt aagctgtttt ttgccacctg agaacagttt
     6361 ctattctggt ccaaaagaaa tattagacta agtttattat aaacgaggct ggtgatctta
     6421 atagctcctc gagggggggc ccggtaccca gcttttgttc cctttagtga gggttaattg
     6481 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6541 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6601 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6661 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6721 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6781 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6841 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6901 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6961 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7021 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7081 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7141 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7201 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7261 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7321 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     7381 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7441 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7501 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7561 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7621 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7681 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7741 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7801 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7861 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7921 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7981 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8041 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8101 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8161 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgttgcgcaa cgttgttgcc
     8221 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8281 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8341 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8401 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8461 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8521 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8581 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8641 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8701 tg
//
