LOCUS       40924_19686       11025 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mini Tn5 transposon vector pFAJ1819, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11025)
  AUTHORS   Brennan E, McGuinness M, Dowling DN.
  TITLE     Dual marker mini Tn5 transposon
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11025)
  AUTHORS   Brennan E, McGuinness M, Dowling DN.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2010) Science and Health, Institute of Technology 
            Carlow, Kilkenny Road, Carlow, Ireland
REFERENCE   3  (bases 1 to 11025)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11025)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-SEP-2010) Science and Health, Institute of Technology Carlow, 
            Kilkenny Road, Carlow, Ireland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(398..1255)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1256..1360)
                     /label=AmpR promoter
     CDS             complement(1410..2837)
                     /codon_start=1
                     /label=Tn5 transposase
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     ITISSEGSEAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
     CDS             complement(3097..3813)
                     /codon_start=1
                     /label=Cycle 3 GFP
                     /note="Cycle 3 GFP (Crameri et al., 1996)"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             complement(4200..4916)
                     /codon_start=1
                     /label=Cycle 3 GFP
                     /note="Cycle 3 GFP (Crameri et al., 1996)"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             complement(5661..6452)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(6881..8689)
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     protein_bind    8772..8788
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      complement(8823..9211)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(9832..10200)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(10233..10342)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
       61 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
      121 tgctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
      181 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
      241 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
      301 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac cataaatcaa
      361 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      421 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
      481 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
      541 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
      601 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
      661 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg
      721 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
      781 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
      841 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
      901 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
      961 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     1021 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     1081 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     1141 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     1201 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     1261 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     1321 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     1381 cacctgcaga tctgcaggtc gaccgatcag atcttgatcc cctgcgccat cagatccttg
     1441 gcggcaagaa agccatccag tttactttgc agggcttccc aaccttccca gagggcgccc
     1501 cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg
     1561 taagcccact gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag
     1621 cccagtagct gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtgt
     1681 tccgcttcct ttagcagccc ttgcgccctg agtgcttgcg gcagcgtgaa gctttctctg
     1741 agctgtaaca gcctgaccgc aacaaacgag aggatcgaga ccatccgctc cagattatcc
     1801 ggctcctcca tgcgttgcct ctcggctcct gctccggttt tccatgcctt atggaactcc
     1861 tcgatccgcc agcgatgggt ataaatgtcg atgacgcgca aggcttgggc tagcgactcg
     1921 accggttcgc tggtcagcaa caaccatttc aacggggtct cacccttggg cgggttaatc
     1981 tcctcggcca gcaccgcgtt gagcgtgata ttcccctgtt ttagcgtgat gcgcccactg
     2041 cgcaggctca agctcgcctt gcgggctggt cgatttttac gtttaccgcg tttatccacc
     2101 acgccctttt gcggaatgct gatctgatag ccacccaact ccggttggtt cttcagatgg
     2161 tcgtacagat acaacccaga ctctacgtcc ttgcgtgggt gcttggagcg caccacgaag
     2221 cgctcgttat gcgccagttt gtcctgcaga taagcatgaa tatcggcttc gcggtcacag
     2281 accgcaatca cgttgctcat catgctgccc atgcgtaacc ggctagttgc ggcggctgcc
     2341 agccatttgc cactctcctt ttcatccgca tcggcagggt catccgggcg catccaccac
     2401 tcctgatgca gtaatcctac ggtgcggaat gtggtggcct cgagcaagag aacggagtga
     2461 acccaccatc cgcgggattt atcctgaata gagcccagct tgccaagctc ttcggcgacc
     2521 tggtggcgat aactcaaaga ggtggtgtcc tcaatggcca gcagttcggg aaactcctga
     2581 gccaacttga ctgtttgcat ggcgccagcc tttctgatcg cctcggcaga aacgttggga
     2641 ttgcggataa atcggtaagc gccttcctgc atggcttcac taccctctga tgagatggtt
     2701 attgatttac cagaatattt tgccaattgg gcggcgacgt taaccaagcg ggcagtacgg
     2761 cgaggatcac ccagcgccgc cgaagagaac acagatttag cccagtcggc cgcacgatga
     2821 agagcagaag ttatcatgaa cgttaccatg ttaggaggtc acatggaaga tcagatcctg
     2881 gaaaacggga aaggttccgt tcaggacgct acttgtgtac gggatcggtc gacggatccc
     2941 aagcttcttc tagactgtct cttgatcaga tctggccgcc taggccgcgg ccgcaagctc
     3001 tagaggatcc ccgggtaccg agctcgaatt cattatttgt agagctcatc catgccatgt
     3061 ctagaggatc cccgggtacc gagctcgaat tcattatttg tagagctcat ccatgccatg
     3121 tgtaatccca gcagcagtta caaactcaag aaggaccatg tggtcacgct tttcgttggg
     3181 atctttcgaa agggcagatt gtgtcgacag gtaatggttg tctggtaaaa ggacagggcc
     3241 atcgccaatt ggagtatttt gttgataatg gtctgctagt tgaacggatc catcttcaat
     3301 gttgtggcga attttgaagt tagctttgat tccattcttt tgtttgtctg ccgtgatgta
     3361 tacattgtgt gagttatagt tgtactcgag tttgtgtccg agaatgtttc catcttcttt
     3421 aaaatcaata ccttttaact cgatacgatt aacaagggta tcaccttcaa acttgacttc
     3481 agcacgcgtc ttgtagttcc cgtcatcttt gaaagatata gtgcgttcct gtacataacc
     3541 ttcgggcatg gcactcttga aaaagtcatg ccgtttcata tgatccggat aacgggaaaa
     3601 gcattgaaca ccataagaga aagtagtgac aagtgttggc catggaacag gtagttttcc
     3661 agtagtgcaa ataaatttaa gggtaagctt tccgtatgta gcatcacctt caccctctcc
     3721 actgacagaa aatttgtgcc cattaacatc accatctaat tcaacaagaa ttgggacaac
     3781 tccagtgaaa agttcttctc ctttgctagc catatgtata tcctccttag ctagtcaggt
     3841 accatcctgt ctcttgatca gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga
     3901 aagccatcca gtttactttg cagggcttcc caaccttacc agagggcgcc ccagctggca
     3961 attccggttc gcttgctgtc cataaaaccg cccagtctag ctatcgccat gtaagcccac
     4021 tgcaagctac ctgctttctc tttgcgcttg cgttttccct tgtccagata gcccagtagc
     4081 tgacattcat ccggggtcag caccgtttct gcggactggc tttctacgtg ttccgcttcc
     4141 tttagcagcc cttgcgccct gagtgcttgc ggcagcgtgt ctagagcttg aattcattat
     4201 ttgtagagct catccatgcc atgtgtaatc ccagcagcag ttacaaactc aagaaggacc
     4261 atgtggtcac gcttttcgtt gggatctttc gaaagggcag attgtgtcga caggtaatgg
     4321 ttgtctggta aaaggacagg gccatcgcca attggagtat tttgttgata atggtctgct
     4381 agttgaacgg atccatcttc aatgttgtgg cgaattttga agttagcttt gattccattc
     4441 ttttgtttgt ctgccgtgat gtatacattg tgtgagttat agttgtactc gagtttgtgt
     4501 ccgagaatgt ttccatcttc tttaaaatca atacctttta actcgatacg attaacaagg
     4561 gtatcacctt caaacttgac ttcagcacgc gtcttgtagt tcccgtcatc tttgaaagat
     4621 atagtgcgtt cctgtacata accttcgggc atggcactct tgaaaaagtc atgccgtttc
     4681 atatgatccg gataacggga aaagcattga acaccataag agaaagtagt gacaagtgtt
     4741 ggccatggaa caggtagttt tccagtagtg caaataaatt taagggtaag ctttccgtat
     4801 gtagcatcac cttcaccctc tccactgaca gaaaatttgt gcccattaac atcaccatct
     4861 aattcaacaa gaattgggac aactccagtg aaaagttctt ctcctttgct agccatatgt
     4921 atatcctcct tagctagtca ggtaccatcc tgtctcttga tcagatcttg atcccctgcg
     4981 ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt
     5041 accagagggc gccccagctg gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc
     5101 tagctatcgc catgtaagcc cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc
     5161 ccttgtccag atagcccagt agctgacatt catccggggt cagcaccgtt tctgcggact
     5221 ggctttctac gtgttccgct tcctttagca gcccttgcgc cctgagtgct tgcggcagcg
     5281 tgtctagagg tacctcaggg gctcgatccc ctcgcgagtt ggttcagctg ctgcctgagg
     5341 ctggacgacc tcgcggagtt ctaccggcag tgcaaatccg tcggcatcca ggaaaccagc
     5401 agcggctatc cgcgcatcca tgcccccgaa ctgcaggagt ggggaggcac gatggccgct
     5461 ttggtcgacc ggggatcgat ccccggggtg ggcgaagaac tccagcatga gatccccgcg
     5521 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
     5581 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc
     5641 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     5701 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     5761 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc
     5821 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     5881 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg
     5941 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     6001 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     6061 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     6121 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     6181 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     6241 gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg
     6301 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     6361 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     6421 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga
     6481 tcagatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact
     6541 ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct
     6601 gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt
     6661 ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt catccggggt
     6721 cagcaccgtt tctgcggact ggctttctac gtgttccgct tcctttagca gcccttgcgc
     6781 cctgagtgct tgcggcagcg tgaagcttgc atgcctgcag gtcgacgagc tcgaattcct
     6841 gcaggtcgac tctagaggat ccccgggtac cgttgattca ttgtttgcct ccctgctgcg
     6901 gtttttcacc gaagttcatg ccagtccagc gtttttgcag cagaaaagcc gccgacttcg
     6961 gtttgcggtc gcgagtgaag atccctttct tgttaccgcc aacgcgcaat atgccttgcg
     7021 aggtcgcaaa atcggcgaaa ttccatacct gttcaccgac gacggcgctg acgcgatcaa
     7081 agacgcggtg atacatatcc agccatgcac actgatactc ttcactccac atgtcggtgt
     7141 acattgagtg cagcccggct aacgtatcca cgccgtattc ggtgatgata atcggctgat
     7201 gcagtttctc ctgccaggcc agaagttctt tttccagtac cttctctgcc gtttccaaat
     7261 cgccgctttg gacataccat ccgtaataac ggttcaggca cagcacatca aagagatcgc
     7321 tgatggtatc ggtgtgagcg tcgcagaaca ttacattgac gcaggtgatc ggacgcgtcg
     7381 ggtcgagttt acgcgttgct tccgccagtg gcgcgaaata ttcccgtgca ccttgcggac
     7441 gggtatccgg ttcgttggca atactccaca tcaccacgct tgggtggttt ttgtcacgcg
     7501 ctatcagctc tttaatcgcc tgtaagtgcg cttgctgagt ttccccgttg actgcctctt
     7561 cgctgtacag ttctttcggc ttgttgcccg cttcgaaacc aatgcctaaa gagaggttaa
     7621 agccgacagc agcagtttca tcaatcacca cgatgccatg ttcatctgcc cagtcgagca
     7681 tctcttcagc gtaagggtaa tgcgaggtac ggtaggagtt ggccccaatc cagtccatta
     7741 atgcgtggtc gtgcaccatc agcacgttat cgaatccttt gccacgcaag tccgcatctt
     7801 catgacgacc aaagccagta aagtagaacg gtttgtggtt aatcaggaac tgttcgccct
     7861 tcactgccac tgaccggatg ccgacgcgaa gcgggtagat atcacactct gtctggcttt
     7921 tggctgtgac gcacagttca tagagataac cttcacccgg ttgccagagg tgcggattca
     7981 ccacttgcaa agtcccgcta gtgccttgtc cagttgcaac cacctgttga tccgcatcac
     8041 gcagttcaac gctgacatca ccattggcca ccacctgcca gtcaacagac gcgtggttac
     8101 agtcttgcgc gacatgcgtc accacggtga tatcgtccac ccaggtgttc ggcgtggtgt
     8161 agagcattac gctgcgatgg attccggcat agttaaagaa atcatggaag taagactgct
     8221 ttttcttgcc gttttcgtcg gtaatcacca ttcccggcgg gatagtctgc cagttcagtt
     8281 cgttgttcac acaaacggtg atacgtacac ttttcccggc aataacatac ggcgtgacat
     8341 cggcttcaaa tggcgtatag ccgccctgat gctccatcac ttcctgatta ttgacccaca
     8401 ctttgccgta atgagtgacc gcatcgaaac gcagcacgat acgctggcct gcccaacctt
     8461 tcggtataaa gacttcgcgc tgataccaga cgttgcccgc ataattacga atatctgcat
     8521 cggcgaactg atcgttaaaa ctgcctggca cagcaattgc ccggctttct tgtaacgcgc
     8581 tttcccacca acgctgatca attccacagt tttcgcgatc cagactgaat gcccacaggc
     8641 cgtcgagttt tttgatttca cgggttgggg tttctacagg acgtaacata agggactcct
     8701 ccttagctag tcaggtaccg agctcgaatt cgcgcggccg cacttgtgta taagagtcag
     8761 aattccacaa attgttatcc gctcacaatt ccacatgtgg aattccacat gtggaattcc
     8821 catgtcagcc gttaagtgtt cctgtgtcac tcaaaattgc tttgagaggc tctaagggct
     8881 tctcagtgcg ttacatccct ggcttgttgt ccacaaccgt taaaccttaa aagctttaaa
     8941 agccttatat attctttttt ttcttataaa acttaaaacc ttagaggcta tttaagttgc
     9001 tgatttatat taattttatt gttcaaacat gagagcttag tacgtgaaac atgagagctt
     9061 agtacgttag ccatgagagc ttagtacgtt agccatgagg gtttagttcg ttaaacatga
     9121 gagcttagta cgttaaacat gagagcttag tacgtgaaac atgagagctt agtacgtact
     9181 atcaacaggt tgaactgctg atcttcagat cctctacgcc ggacgcatcg tggccggatc
     9241 cagccgacca ggctttccac gcccgcgtgc cgctccatgt cgttcgcgcg gttctcggaa
     9301 acgcgctgcc gcgtttcgtg attgtcacgc tcaagcccgt agtcccgttc gagcgtcgcg
     9361 cagaggtcag cgagggcgcg gtaggcccga tacggctcat ggatggtgtt tcgggtcggg
     9421 tgaatcttgt tgatggcgat atggatgtgc aggttgtcgg tgtcgtgatg cacggcactg
     9481 acgcgctgat gctcggcgaa gccaagccca gcgcagatgc ggtcctcaat cgcgcgcaac
     9541 gtctccgcgt cgggcttctc tcccgcgcgg aagctaacca gcaggtgata ggtcttgtcg
     9601 gcctcggaac gggtgttgcc gtgctgggtc gccatcacct cggccatgac agcgggcagg
     9661 gtgtttgcct cgcagttcgt gacgcgcacg tgacccaggc gctcggtctt gccttgctcg
     9721 tcggtgatgt acttcaccag ctccgcgaag tcgctcttct tgatggagcg catggggacg
     9781 tgcttggcaa tcacgcgcac cccccggccg ttttagcggc taaaaaagtc atggctctgc
     9841 cctcgggcgg accacgccca tcatgacctt gccaagctcg tcctgcttct cttcgatctt
     9901 cgccagcagg gcgaggatcg tggcatcacc gaaccgcgcc gtgcgcgggt cgtcggtgag
     9961 ccagagtttc agcaggccgc ccaggcggcc caggtcgcca ttgatgcggg ccagctcgcg
    10021 gacgtgctca tagtccacga cgcccgtgat tttgtagccc tggccgacgg ccagcaggta
    10081 ggccgacagg ctcatgccgg ccgccgccgc cttttcctca atcgctcttc gttcgtctgg
    10141 aaggcagtac accttgatag gtgggctgcc cttcctggtt ggcttggttt catcagccat
    10201 ccgcttgccc tcatctgtta cgccggcggt agccggccag cctcgcagag caggattccc
    10261 gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg
    10321 gcctacttca cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg
    10381 aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc
    10441 tataatgacc ccgaagcagg gttatgcagc ggaaaagcgc tgcttccctg ctgttttgtg
    10501 gaatatctac cgactggaaa caggcaaatg caggaaatta ctgaactgag gggacaggcg
    10561 agagacgatg ccaaagagct acaccgacga gctggccgag tgggttgaat cccgcgcggc
    10621 caagaagcgc cggcgtgatg aggctgcggt tgcgttcctg gcggtgaggg cggatgtcga
    10681 ggcggcgtta gcgtccggct atgcgctcgt caccatttgg gagcacatgc gggaaacggg
    10741 gaaggtcaag ttctcctacg agacgttccg ctcgcacgcc aggcggcaca tcaaggccaa
    10801 gcccgccgat gtgcccgcac cgcaggccaa ggctgcggaa cccgcgccgg cacccaagac
    10861 gccggagcca cggcggccga agcagggggg caaggctgaa aagccggccc ccgctgcggc
    10921 cccgaccggc ttcaccttca acccaacacc ggacaaaaag gatcctctac gccggacgca
    10981 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcct
//
