LOCUS       40924_19681        4980 bp DNA     circular SYN 18-DEC-2018
DEFINITION  BiFC vector pFAGN173, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Gong Z, Lu H.
  TITLE     A new combination of fluorescent protein fragments suitable for 
            green-colored bimolecular fluorescence complementation (BiFC) assays
            in Schizosaccharomyces pombe
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Gong Z, Lu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2012) School of Life Science, Fudan University, 
            Room 618, Science Building, Handan Road 220, Shanghai 200433, China
REFERENCE   3  (bases 1 to 4980)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4980)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-DEC-2012) School of Life Science, Fudan University, Room 618, 
            Science Building, Handan Road 220, Shanghai 200433, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        235..253
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    303..317
                     /label=linker
                     /note="linker"
     CDS             318..830
                     /label=VN173
                     /note="N-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
     CDS             831..857
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     regulatory      867..1359
                     /label=Saccharomyces cerevisiae LEU2 terminator
                     /note="Saccharomyces cerevisiae LEU2 terminator"
                     /regulatory_class="terminator"
     gene            1405..2761
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     promoter        complement(2869..2887)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3145..3733)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3907..4764)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4765..4869)
                     /label=AmpR promoter
ORIGIN
        1 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
       61 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
      121 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tggacatatt
      181 gtcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag
      241 gtgacactat agaacgcggc cgccagctga agcttcgtac gctgcaggtc gaccccggta
      301 cccgttccat tgctaccagt aaaggagaag aacttttcac tggagttgtc ccaattcttg
      361 ttgaattaga tggtgacgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
      421 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
      481 catggccaac acttgtcact actctgactt atggtgttca atgcttttca agatacccag
      541 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
      601 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
      661 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
      721 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
      781 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa tacccttacg
      841 atgttcccga ctacgcttaa tctagagaaa atccttgctt aaaaagattc tcttttttta
      901 tgatatttgt acataaactt tataaatgaa attcataata gaaacgacac gaaattacaa
      961 aatggaatat gttcataggg tagacgaaac tatatacgca atctacatac atttatcaag
     1021 aaggagaaaa aggaggatgt aaaggaatac aggtaagcaa attgatacta atggctcaac
     1081 gtgataagga aaaagaattg cactttaaca ttaatattga caaggaggag ggcaccacac
     1141 aaaaagttag gtgtaacaga aaatcatgaa actatgattc ctaatttata tattggagga
     1201 ttttctctaa aaaaaaaaaa atacaacaaa taaaaaacac tcaatgacct gaccatttga
     1261 tggagtttaa gtcaatacct tcttgaacca tttcccataa tggtgaaagt tccctcaaga
     1321 attttactct gtcagaaacg gccttaacga cgtagtcgaa gatctgttta gcttgcctcg
     1381 tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt
     1441 gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc
     1501 catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac
     1561 ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt
     1621 ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc
     1681 tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat
     1741 aaacaaccat gggtaaggaa aagactcacg tttcgaggcc gcgattaaat tccaacatgg
     1801 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     1861 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     1921 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     1981 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     2041 cgatccccgg caaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     2101 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     2161 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     2221 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     2281 aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     2341 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     2401 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     2461 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     2521 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agtactgaca ataaaaagat
     2581 tcttgttttc aagaacttgt catttgtata gtttttttat attgtagttg ttctatttta
     2641 atcaaatgtt agcgtgattt atattttttt tcgcctcgac atcatctgcc cagatgcgaa
     2701 gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg tcgctatact
     2761 gctgtcgatt cgatactaac gccgccatcc agtgtcgaaa acgagctcga attcatcgat
     2821 gatatcagat ccactagtgg cctatgcggc cgcggatctg ccggtctccc tatagtgagt
     2881 cgtattaatt tcgataagcc aggttaacct gcattaatga atcggccaac gcgcggggag
     2941 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     3001 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3061 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3121 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3181 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3241 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3301 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     3361 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3421 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3481 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3541 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     3601 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3661 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3721 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3781 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3841 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3901 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3961 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     4021 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     4081 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4141 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4201 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4261 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4321 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4381 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4441 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4501 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4561 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4621 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4681 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4741 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4801 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4861 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     4921 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
//
