LOCUS       Exported                3701 bp DNA     circular SYN 05-JUN-2025
DEFINITION  Plasmid for yeast gene deletion using the natMX6 selectable marker 
            conferring nourseothricin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Hentges P, Van Driessche B, Tafforeau L, Vandenhaute J, Carr AM.
  TITLE     Three novel antibiotic marker cassettes for gene disruption and 
            marker switching in Schizosaccharomyces pombe.
  JOURNAL   Yeast 2005;22:1013-9.
  PUBMED    16200533
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   EUROSCARF
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3701)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3701)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3701)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2005"; volume: "22"; pages: "1013-9"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            113..1232
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        113..456
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             457..1029
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      1035..1232
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(1337..1355)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1613..2201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2372..3232)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3233..3337)
                     /label=AmpR promoter
     promoter        3683..3701
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 acgcggccgc cagctgaagc ttcgtacgct gcaggtcgac ggatccccgg gttaattaag
       61 gcgcgccaga tctgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga
      121 ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg
      181 tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga
      241 tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg agcagggaaa
      301 cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa
      361 aggttaggat ttgccactga ggttcttctt tcatatactt cctttttaaa tcttgctagg
      421 atacagttct cacatcacat ccgaacataa acaaccatgg gtaccactct tgacgacacg
      481 gcttaccggt accgcaccag tgtcccgggg gacgccgagg ccatcgaggc actggatggg
      541 tccttcacca ccgacaccgt cttccgcgtc accgccaccg gggacggctt caccctgcgg
      601 gaggtgccgg tggacccgcc cctgaccaag gtgttccccg acgacgaatc ggacgacgaa
      661 tcggacgacg gggaggacgg cgacccggac tctcggacgt tcgtcgcgta cggggacgac
      721 ggcgacctgg cgggcttcgt ggtcgtctcg tactccggct ggaaccgccg gctgaccgtc
      781 gaggacatcg aggtcgcccc ggagcaccgg gggcacgggg tcgggcgcgc gttgatgggg
      841 ctcgcgacgg agttcgcccg cgagcggggt gccgggcacc tctggctgga ggtcaccaac
      901 gtcaacgcac cggcgatcca cgcgtaccgg cggatggggt tcaccctctg cggcctggac
      961 accgccctgt acgacggcac cgcctcggac ggcgagcagg cgctctacat gagcatgccc
     1021 tgcccctaat cagtactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat
     1081 agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt
     1141 ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta atatcatgcg
     1201 tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa cgccgccatc
     1261 cagtttaaac gagctcgaat tcatcgatga tatcagatcc actagtggcc tatgcggccg
     1321 cggatctgcc ggtctcccta tagtgagtcg tattaatttc gataagccag gttaacctgc
     1381 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1441 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1501 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1561 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1621 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1681 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1741 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1801 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1861 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1921 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1981 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2041 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2101 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2161 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2221 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2281 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2341 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2401 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2461 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2521 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2581 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2641 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2701 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2761 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2821 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2881 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2941 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3001 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3061 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3121 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3181 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3241 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3301 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3361 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3421 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     3481 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     3541 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     3601 tactgagagt gcaccatatg gacatattgt cgttagaacg cggctacaat taatacataa
     3661 ccttatgtat catacacata cgatttaggt gacactatag a
//
