LOCUS       40924_19516        4653 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast tagging vector pFA6a-myc-KlURA3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Sung M-K., Huh W-K.
  TITLE     Yeast tagging vector pFA6a-myc-KlURA3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Sung M-K., Huh W-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2007) School of Biological Sciences, Seoul 
            National University, Seoul 151-747, Republic of Korea
REFERENCE   3  (bases 1 to 4653)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4653)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-OCT-2007) School of Biological Sciences, Seoul National 
            University, Seoul 151-747, Republic of Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             66..95
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             102..131
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             147..176
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             183..212
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             219..248
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             890..1693
                     /codon_start=1
                     /gene="URA3"
                     /product="orotidine 5'-phosphate decarboxylase"
                     /label=URA3
                     /protein_id="ABX47175.1"
                     /translation="MSTKSYTSRAETHASPVASKLLRLMDEKKTNLCASLDVRSTDELL
                     KLVETLGPYICLLKTHVDILDDFSYEGTVVPLKALAEKYKFLIFEDRKFADIGNTVKLQ
                     YTSGVYRIAEWSDITNAHGVTGAGIVAGLKQGAQEVTKEPRGLLMLAELSSKGSLAHGE
                     YTKGTVDIAKSDKDFVIGFIAQNDMGGREEGFDWLIMTPGVGLDDKGDALGQQYRTVDE
                     VVSGGSDIIIVGRGLFAKGRDPKVEGERYRNAGWEAYQKRISAPH"
     gene            890..1693
                     /gene="URA3"
                     /label=URA3
     terminator      1989..2186
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(2291..2309)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2567..3155)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3329..4186)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4187..4291)
                     /label=AmpR promoter
     promoter        4637..4653
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 acggtgaaca aaagctaatc tccgaggaag acttgaacgg tgaacaaaaa ttaatctcag
      121 aagaagactt gaacggactc gacggtgaac aaaagttgat ttctgaagaa gatttgaacg
      181 gtgaacaaaa gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag
      241 aagacttgaa ttgaggcgcg cctctctgct tttgtgcgcg tatgtttatg tatgtacctc
      301 tctctctatt tctattttta aaccaccctc tcaataaaat aaaaataata aagtattttt
      361 aaggaaaaga cgtgtttaag cactgacttt atctactttt tgtacgtttt cattgatata
      421 atgtgttttg tctctccctt ttctacgaaa atttcaaaaa ttgaccaaaa aaaggaatat
      481 atatacgaaa aactattata tttatatatc atagtgttga taaaaaatgt ttatcagatc
      541 tcggagacaa tcatatggga gaagcaattg gaagatagaa aaaaggtact cggtacataa
      601 atatatgtga ttctgggtag aagatcggtc tgcattggat ggtggtaacg cattttttta
      661 cacacattac ttgcctcgag catcaaatgg tggttattcg tggatctata tcacgtgatt
      721 tgcttaagaa ttgtcgttca tggtgacact tttagctttg acatgattaa gctcatctca
      781 attgatgtta tctaaagtca tttcaactat ctaagatgtg gttgtgattg ggccattttg
      841 tgaaagccag tacgccagcg tcaatacact cccgtcaatt agttgcacca tgtccacaaa
      901 atcatatacc agtagagctg agactcatgc aagtccggtt gcatcgaaac ttttacgttt
      961 aatggatgaa aagaagacca atttgtgtgc ttctcttgac gttcgttcga ctgatgagct
     1021 attgaaactt gttgaaacgt tgggtccata catttgcctt ttgaaaacac acgttgatat
     1081 cttggatgat ttcagttatg agggtactgt cgttccattg aaagcattgg cagagaaata
     1141 caagttcttg atatttgagg acagaaaatt cgccgatatc ggtaacacag tcaaattaca
     1201 atatacatcg ggcgtttacc gtatcgcaga atggtctgat atcaccaacg cccacggggt
     1261 tactggtgct ggtattgttg ctggcttgaa acaaggtgcg caagaggtca ccaaagaacc
     1321 aaggggatta ttgatgcttg ctgaattgtc ttccaagggt tctctagcac acggtgaata
     1381 tactaagggt accgttgata ttgcaaagag tgataaagat ttcgttattg ggttcattgc
     1441 tcagaacgat atgggaggaa gagaagaagg gtttgattgg ctaatcatga ccccaggtgt
     1501 aggtttagac gacaaaggcg atgcattggg tcagcagtac agaaccgtcg acgaagttgt
     1561 aagtggtgga tcagatatca tcattgttgg cagaggactt ttcgccaagg gtagagatcc
     1621 taaggttgaa ggtgaaagat acagaaatgc tggatgggaa gcgtaccaaa agagaatcag
     1681 cgctccccat taattataca ggaaacttaa tagaacaaat cacatattta atctaatagc
     1741 cacctgcatt ggcacggtgc aacactcact tcaacttcat cttacaaaag atcacgtgat
     1801 ctgttgtatt gaactgaaaa ttttttgttt gcttctctct ctctctttca ttatgtgaga
     1861 gtttaaaaac cagaaactac atcatcgaaa aagagtttaa accattacaa ccattgcgat
     1921 aagccctctc aaacttcctc cagaaccaat gacgttccct caaccaaagg tgttttgatg
     1981 tgaagagtac tgacaataaa aagattcttg ttttcaagaa cttgtcattt gtatagtttt
     2041 tttatattgt agttgttcta ttttaatcaa atgttagcgt gatttatatt ttttttcgcc
     2101 tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa agtaatatca tgcgtcaatc
     2161 gtatgtgaat gctggtcgct atactgctgt cgattcgata ctaacgccgc catccagttt
     2221 aaacgagctc gaattcatcg atgatatcag atccactagt ggcctatgcg gccgcggatc
     2281 tgccggtctc cctatagtga gtcgtattaa tttcgataag ccaggttaac ctgcattaat
     2341 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     2401 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2461 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2521 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2581 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2641 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2701 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2761 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2821 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2881 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2941 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3001 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3061 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3121 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3181 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3241 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3301 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3361 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3421 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3481 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3541 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3601 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3661 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3721 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3781 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3841 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     3901 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     3961 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4021 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4081 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4141 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4201 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4261 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     4321 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     4381 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     4441 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     4501 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     4561 gagtgcacca tatggacata ttgtcgttag aacgcggcta caattaatac ataaccttat
     4621 gtatcataca catacgattt aggtgacact ata
//
