LOCUS       40924_19491        4485 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid for swapping in the GAL1 promoter using the kanMX selectable
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 4485)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4485)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(87..528)
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     gene            662..2018
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     promoter        complement(2123..2141)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2399..2987)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3161..4018)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4019..4123)
                     /label=AmpR promoter
     promoter        4469..4485
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 aagacatttt gagatccggg ttttttctcc ttgacgttaa agtatagagg tatattaaca
      121 attttttgtt gatactttta ttacatttga ataagaagta atacaaaccg aaaatgttga
      181 aagtattagt taaagtggtt atgcagtttt tgcatttata tatctgttaa tagatcaaaa
      241 atcatcgctt cgctgattaa ttaccccaga aataaggcta aaaaactaat cgcattatca
      301 tcctatggtt gttaatttga ttcgttcatt tgaaggtttg tggggccagg ttactgccaa
      361 tttttcctct tcataaccat aaaagctagt attgtagaat ctttattgtt cggagcagtg
      421 cggcgcgagg cacatctgcg tttcaggaac gcgaccggtg aagacgagga cgcacggagg
      481 agagtcttcc ttcggagggc tgtcacccgc tcggcggctt ctaatccgta cttcaatata
      541 gcaatgagca gttaagcgta ttactgaaag ttccaaagag aaggtttttt taggctaaga
      601 taatggggct ctttacagat ctgtttagct tgcctcgtcc ccgccgggtc acccggccag
      661 cgacatggag gcccagaata ccctccttga cagtcttgac gtgcgcagct caggggcatg
      721 atgtgactgt cgcccgtaca tttagcccat acatccccat gtataatcat ttgcatccat
      781 acattttgat ggccgcacgg cgcgaagcaa aaattacggc tcctcgctgc agacctgcga
      841 gcagggaaac gctcccctca cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag
      901 aaatataaaa ggttaggatt tgccactgag gttcttcttt catatacttc cttttaaaat
      961 cttgctagga tacagttctc acatcacatc cgaacataaa caaccatggg taaggaaaag
     1021 actcacgttt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     1081 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc
     1141 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     1201 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     1261 atccgtactc ctgatgatgc atggttactc accactgcga tccccggcaa aacagcattc
     1321 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     1381 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     1441 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     1501 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca
     1561 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     1621 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     1681 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     1741 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     1801 gatgagtttt tctaatcagt actgacaata aaaagattct tgttttcaag aacttgtcat
     1861 ttgtatagtt tttttatatt gtagttgttc tattttaatc aaatgttagc gtgatttata
     1921 ttttttttcg cctcgacatc atctgcccag atgcgaagtt aagtgcgcag aaagtaatat
     1981 catgcgtcaa tcgtatgtga atgctggtcg ctatactgct gtcgattcga tactaacgcc
     2041 gccatccagt ttaaacgagc tcgaattcat cgatgatatc agatccacta gtggcctatg
     2101 cggccgcgga tctgccggtc tccctatagt gagtcgtatt aatttcgata agccaggtta
     2161 acctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     2221 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2281 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2341 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2401 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2461 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2521 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2581 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2641 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2701 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2761 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2821 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2881 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2941 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3001 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3061 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3121 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3181 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3241 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3301 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3361 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3421 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3481 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3541 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3601 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3661 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3721 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3781 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3841 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3901 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3961 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     4021 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     4081 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4141 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     4201 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     4261 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     4321 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     4381 agattgtact gagagtgcac catatggaca tattgtcgtt agaacgcggc tacaattaat
     4441 acataacctt atgtatcata cacatacgat ttaggtgaca ctata
//
