LOCUS       40924_19476        5000 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFA6a-kanMX6-PGAL1-HBH, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, 
            Auer B, Huang L, Kaiser P.
  TITLE     HB tag modules for PCR-based gene tagging and tandem affinity 
            purification in Saccharomyces cerevisiae
  JOURNAL   Yeast 23 (8), 623-632 (2006)
  PUBMED    16823883
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Kaiser P, Tagwerker C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2006) Biological Chemistry, UC Irvine, 19182 
            Jamboree Blvd RM H136 Plumwood Bldg, Irvine, CA 92697, USA
REFERENCE   3  (bases 1 to 5000)
  AUTHORS   Kaiser P, Tagwerker C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2009) Biological Chemistry, UC Irvine, 19182 
            Jamboree Blvd RM H136 Plumwood Bldg, Irvine, CA 92697, USA
REFERENCE   4  (bases 1 to 5000)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5000)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2006"; volume: "23"; issue: "8"; pages: "623-632"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-FEB-2006) Biological Chemistry, UC Irvine, 19182 Jamboree Blvd 
            RM H136 Plumwood Bldg, Irvine, CA 92697, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (21-AUG-2009) Biological Chemistry, UC Irvine, 19182 Jamboree Blvd 
            RM H136 Plumwood Bldg, Irvine, CA 92697, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     On Aug 21, 2009 this sequence version replaced DQ407930.1.
FEATURES             Location/Qualifiers
     source          1..5000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..19)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        365..469
                     /label=AmpR promoter
     CDS             470..1327
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1501..2089
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2347..2365
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            complement(2470..3826)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     promoter        3960..4401
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     CDS             4448..4465
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             4694..4711
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      4738..4925
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
ORIGIN
        1 tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc
       61 gcgttctaac gacaatatgt ccatatggtg cactctcagt acaatctgct ctgatgccgc
      121 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
      181 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
      241 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
      301 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
      361 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
      421 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
      481 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
      541 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
      601 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
      661 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
      721 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
      781 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
      841 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
      901 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
      961 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     1021 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     1081 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     1141 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     1201 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     1261 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     1321 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     1381 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     1441 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1501 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1561 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     1621 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     1681 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1741 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1801 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     1861 ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg
     1921 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     1981 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2041 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2101 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     2161 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     2221 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     2281 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaggttaa cctggcttat
     2341 cgaaattaat acgactcact atagggagac cggcagatcc gcggccgcat aggccactag
     2401 tggatctgat atcatcgatg aattcgagct cgtttaaact ggatggcggc gttagtatcg
     2461 aatcgacagc agtatagcga ccagcattca catacgattg acgcatgata ttactttctg
     2521 cgcacttaac ttcgcatctg ggcagatgat gtcgaggcga aaaaaaatat aaatcacgct
     2581 aacatttgat taaaatagaa caactacaat ataaaaaaac tatacaaatg acaagttctt
     2641 gaaaacaaga atctttttat tgtcagtact gattagaaaa actcatcgag catcaaatga
     2701 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     2761 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     2821 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     2881 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta
     2941 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     3001 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg
     3061 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     3121 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttg
     3181 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     3241 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
     3301 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     3361 aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     3421 aaatcagcat ccatgttgga atttaatcgc ggcctcgaaa cgtgagtctt ttccttaccc
     3481 atggttgttt atgttcggat gtgatgtgag aactgtatcc tagcaagatt ttaaaaggaa
     3541 gtatatgaaa gaagaacctc agtggcaaat cctaaccttt tatatttctc tacaggggcg
     3601 cggcgtgggg acaattcaac gcgtctgtga ggggagcgtt tccctgctcg caggtctgca
     3661 gcgaggagcc gtaatttttg cttcgcgccg tgcggccatc aaaatgtatg gatgcaaatg
     3721 attatacatg gggatgtatg ggctaaatgt acgggcgaca gtcacatcat gcccctgagc
     3781 tgcgcacgtc aagactgtca aggagggtat tctgggcctc catgtcgctg gccgggtgac
     3841 ccggcgggga cgaggcaagc taaacagatc tgtaaagagc cccattatct tagcctaaaa
     3901 aaaccttctc tttggaactt tcagtaatac gcttaactgc tcattgctat attgaagtac
     3961 ggattagaag ccgccgagcg ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc
     4021 tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac
     4081 aataaagatt ctacaatact agcttttatg gttatgaaga ggaaaaattg gcagtaacct
     4141 ggccccacaa accttcaaat gaacgaatca aattaacaac cataggatga taatgcgatt
     4201 agttttttag ccttatttct ggggtaatta atcagcgaag cgatgatttt tgatctatta
     4261 acagatatat aaatgcaaaa actgcataac cactttaact aatactttca acattttcgg
     4321 tttgtattac ttcttattca aatgtaataa aagtatcaac aaaaaattgt taatatacct
     4381 ctatacttta acgtcaagga gaaaaaaccc ggatctcaaa atgtctttaa ttaacatgag
     4441 gggttcacat catcaccacc atcatgctgg aaaggccggt gaaggtgaaa tccctgcccc
     4501 tcttgctggt accgtttcta agatactggt aaaagaaggt gacactgtta aagctggtca
     4561 aacagttctg gtgctggagg ctatgaaaat ggagacagaa attaacgctc ctactgacgg
     4621 aaaagttgaa aaggtgttag ttaaggaaag agatgctgtt caaggtggtc aaggtctaat
     4681 caagatcggc gttcatcatc accaccatca tggagccggc gcgccacttc taaataagcg
     4741 aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac
     4801 aaattttaaa gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc
     4861 ctgtaggtca ggttgctttc tcaggtatag tatgaggtcg ctcttattga ccacacctct
     4921 accggcagat ccgctaggga taacagggta atatagatcc gtcgacctgc agcgtacgaa
     4981 gcttcagctg gcggccgcgt
//
