LOCUS       pFA6a-kanMX6-PGA        5365 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid with a kanMX marker for swapping in the GAL1 promoter and 
            adding a GST tag.
ACCESSION   .
VERSION     .
KEYWORDS    pFA6a-kanMX6-PGAL1-GST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 5365)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5365)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(50..237)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(279..932)
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             complement(945..947)
                     /codon_start=1
                     /label=start codon
                     /note="start codon"
                     /translation="M"
     promoter        complement(967..1408)
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     gene            1542..2898
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     promoter        complement(3003..3021)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3279..3867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4041..4898)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4899..5003)
                     /label=AmpR promoter
     promoter        5349..5365
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatctgc cggtagaggt
       61 gtggtcaata agagcgacct catgctatac ctgagaaagc aacctgacct acaggaaaga
      121 gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa atttgtatac
      181 acttattttt tttataactt atttaataat aaaaatcata aatcataaga aattcgctta
      241 tttagaagtg gcgcgcctca acgcggaacc agatccgatt ttggaggatg gtcgccacca
      301 ccaaacgtgg cttgccagcc ctgcaaaggc catgctatat acttgctgga tttcaagtac
      361 ttatcaattt gtgggatagc ttcaatacgt tttttaaaac aaactaattt tgggaacgca
      421 tccaggcaca ttgggtccat gtataaaaca acatcaagag cgtcatacaa catgaagtca
      481 ggatgggtta catgatcacc atttaaatat gttttatgac ataaacgatc ttcgaacatt
      541 ttcagcattt caggtagctt gctaagaaaa tcaactttga gagtttcaaa gtctttacta
      601 tatgcaattc tcgaaacacc gtatctaata tccaaaaccg ctccttcaag cattgaaatc
      661 tctgcacgct cttttggaca accacccaac atgttgtgct tgtcagctat ataacgtatg
      721 atggccatag actgtgttaa tttaacatca ccatcaatat aataaggaag attgggaaac
      781 tccaaaccca attcaaactt tttgtttcgc catttatcac cttcatcgcg ctcatacaaa
      841 tgctcttcat atttttcttc aagatattcc aaaagaagtc gagtgggttg cacaaggccc
      901 ttaattttcc aataacctag tataggggac atattaatta aagacatttt gagatccggg
      961 ttttttctcc ttgacgttaa agtatagagg tatattaaca attttttgtt gatactttta
     1021 ttacatttga ataagaagta atacaaaccg aaaatgttga aagtattagt taaagtggtt
     1081 atgcagtttt tgcatttata tatctgttaa tagatcaaaa atcatcgctt cgctgattaa
     1141 ttaccccaga aataaggcta aaaaactaat cgcattatca tcctatggtt gttaatttga
     1201 ttcgttcatt tgaaggtttg tggggccagg ttactgccaa tttttcctct tcataaccat
     1261 aaaagctagt attgtagaat ctttattgtt cggagcagtg cggcgcgagg cacatctgcg
     1321 tttcaggaac gcgaccggtg aagacgagga cgcacggagg agagtcttcc ttcggagggc
     1381 tgtcacccgc tcggcggctt ctaatccgta cttcaatata gcaatgagca gttaagcgta
     1441 ttactgaaag ttccaaagag aaggtttttt taggctaaga taatggggct ctttacagat
     1501 ctgtttagct tgcctcgtcc ccgccgggtc acccggccag cgacatggag gcccagaata
     1561 ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtaca
     1621 tttagcccat acatccccat gtataatcat ttgcatccat acattttgat ggccgcacgg
     1681 cgcgaagcaa aaattacggc tcctcgctgc agacctgcga gcagggaaac gctcccctca
     1741 cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt
     1801 tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc
     1861 acatcacatc cgaacataaa caaccatggg taaggaaaag actcacgttt cgaggccgcg
     1921 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     1981 gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct
     2041 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     2101 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     2161 atggttactc accactgcga tccccggcaa aacagcattc caggtattag aagaatatcc
     2221 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     2281 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     2341 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     2401 tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt
     2461 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     2521 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     2581 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     2641 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcagt
     2701 actgacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt tttttatatt
     2761 gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg cctcgacatc
     2821 atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga
     2881 atgctggtcg ctatactgct gtcgattcga tactaacgcc gccatccagt ttaaacgagc
     2941 tcgaattcat cgatgatatc agatccacta gtggcctatg cggccgcgga tctgccggtc
     3001 tccctatagt gagtcgtatt aatttcgata agccaggtta acctgcatta atgaatcggc
     3061 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     3121 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3181 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3241 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3301 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3361 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3421 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
     3481 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3541 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3601 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3661 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     3721 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3781 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3841 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3901 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3961 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4021 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4081 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4141 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4201 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4261 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4321 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4381 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4441 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4501 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4561 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4621 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4681 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     4741 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     4801 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     4861 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     4921 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4981 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     5041 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     5101 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     5161 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     5221 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     5281 catatggaca tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata
     5341 cacatacgat ttaggtgaca ctata
//
