LOCUS       pFA6a-kanMX6-PGA        5404 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid with a kanMX marker for swapping in the GAL1 promoter and 
            adding a GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    pFA6a-kanMX6-PGAL1-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 5404)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5404)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(50..237)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(261..971)
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
     CDS             complement(984..986)
                     /codon_start=1
                     /label=start codon
                     /note="start codon"
                     /translation="M"
     promoter        complement(1006..1447)
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     gene            1581..2937
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     promoter        complement(3042..3060)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3318..3906)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4080..4937)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4938..5042)
                     /label=AmpR promoter
     promoter        5388..5404
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatctgc cggtagaggt
       61 gtggtcaata agagcgacct catgctatac ctgagaaagc aacctgacct acaggaaaga
      121 gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa atttgtatac
      181 acttattttt tttataactt atttaataat aaaaatcata aatcataaga aattcgctta
      241 tttagaagtg gcgcgcccta tttgtatagt tcatccatgc catgtgtaat cccagcagct
      301 gttacaaact caagaaggac catgtggtct ctcttttcgt tgggatcttt cgaaagggca
      361 gattgtgtgg acaggtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta
      421 ttttgttgat aatggtctgc tagttgaacg cttccatctt caatgttgtg tctaattttg
      481 aagttaactt tgattccatt cttttgtttg tctgccatga tgtatacatt gtgtgagtta
      541 tagttgtatt ccaatttgtg tccaagaatg tttccatctt ctttaaaatc aatacctttt
      601 aactcgattc tattaacaag ggtatcacct tcaaacttga cttcagcacg tgtcttgtag
      661 ttcccgtcat ctttgaaaaa tatagttctt tcctgtacat aaccttcggg catggcactc
      721 ttgaaaaagt catgccgttt catatgatct gggtatcttg aaaagcattg aacaccatac
      781 gtaaaagtag tgacaagtgt tggccatgga acaggtagtt ttccagtagt gcaaataaat
      841 ttaagggtaa gttttccgta tgttgcatca ccttcaccct ctccactgac agaaaatttg
      901 tgcccattaa catcaccatc taattcaaca agaattggga caactccagt gaaaagttct
      961 tctcctttac tgttaattaa agacattttg agatccgggt tttttctcct tgacgttaaa
     1021 gtatagaggt atattaacaa ttttttgttg atacttttat tacatttgaa taagaagtaa
     1081 tacaaaccga aaatgttgaa agtattagtt aaagtggtta tgcagttttt gcatttatat
     1141 atctgttaat agatcaaaaa tcatcgcttc gctgattaat taccccagaa ataaggctaa
     1201 aaaactaatc gcattatcat cctatggttg ttaatttgat tcgttcattt gaaggtttgt
     1261 ggggccaggt tactgccaat ttttcctctt cataaccata aaagctagta ttgtagaatc
     1321 tttattgttc ggagcagtgc ggcgcgaggc acatctgcgt ttcaggaacg cgaccggtga
     1381 agacgaggac gcacggagga gagtcttcct tcggagggct gtcacccgct cggcggcttc
     1441 taatccgtac ttcaatatag caatgagcag ttaagcgtat tactgaaagt tccaaagaga
     1501 aggttttttt aggctaagat aatggggctc tttacagatc tgtttagctt gcctcgtccc
     1561 cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac agtcttgacg
     1621 tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata catccccatg
     1681 tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa aattacggct
     1741 cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg aattgtcccc
     1801 acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg ttcttctttc
     1861 atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc gaacataaac
     1921 aaccatgggt aaggaaaaga ctcacgtttc gaggccgcga ttaaattcca acatggatgc
     1981 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     2041 tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     2101 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     2161 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     2221 ccccggcaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     2281 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     2341 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     2401 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     2461 aatgcataag cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     2521 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     2581 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     2641 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     2701 gcagtttcat ttgatgctcg atgagttttt ctaatcagta ctgacaataa aaagattctt
     2761 gttttcaaga acttgtcatt tgtatagttt ttttatattg tagttgttct attttaatca
     2821 aatgttagcg tgatttatat tttttttcgc ctcgacatca tctgcccaga tgcgaagtta
     2881 agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg
     2941 tcgattcgat actaacgccg ccatccagtt taaacgagct cgaattcatc gatgatatca
     3001 gatccactag tggcctatgc ggccgcggat ctgccggtct ccctatagtg agtcgtatta
     3061 atttcgataa gccaggttaa cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3121 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3181 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3241 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3301 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3361 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3421 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3481 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     3541 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3601 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3661 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3721 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     3781 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3841 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3901 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3961 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4021 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4081 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4141 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4201 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     4261 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4321 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4381 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4441 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4501 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4561 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4621 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4681 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4741 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4801 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     4861 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     4921 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     4981 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5041 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     5101 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     5161 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     5221 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     5281 aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat attgtcgtta
     5341 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
     5401 tata
//
