LOCUS       pFA6a-kanMX6-PGA        4798 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid with a kanMX marker for swapping in the GAL1 promoter and 
            adding a triple-HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    pFA6a-kanMX6-PGAL1-3HA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 4798)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4798)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(50..237)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(270..359)
                     /label=3xHA
                     /note="three tandem HA epitope tags"
     CDS             complement(378..380)
                     /codon_start=1
                     /label=start codon
                     /note="start codon"
                     /translation="M"
     promoter        complement(400..841)
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     gene            975..2331
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     promoter        complement(2436..2454)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2712..3300)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3474..4331)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4332..4436)
                     /label=AmpR promoter
     promoter        4782..4798
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatctgc cggtagaggt
       61 gtggtcaata agagcgacct catgctatac ctgagaaagc aacctgacct acaggaaaga
      121 gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa atttgtatac
      181 acttattttt tttataactt atttaataat aaaaatcata aatcataaga aattcgctta
      241 tttagaagtg gcgcgcctca gcactgagca gcgtaatctg gaacgtcata tggataggat
      301 cctgcatagt ccgggacgtc atacggatag cccgcatagt caggaacatc gtatgggtaa
      361 aagatgttaa ttaaagacat tttgagatcc gggttttttc tccttgacgt taaagtatag
      421 aggtatatta acaatttttt gttgatactt ttattacatt tgaataagaa gtaatacaaa
      481 ccgaaaatgt tgaaagtatt agttaaagtg gttatgcagt ttttgcattt atatatctgt
      541 taatagatca aaaatcatcg cttcgctgat taattacccc agaaataagg ctaaaaaact
      601 aatcgcatta tcatcctatg gttgttaatt tgattcgttc atttgaaggt ttgtggggcc
      661 aggttactgc caatttttcc tcttcataac cataaaagct agtattgtag aatctttatt
      721 gttcggagca gtgcggcgcg aggcacatct gcgtttcagg aacgcgaccg gtgaagacga
      781 ggacgcacgg aggagagtct tccttcggag ggctgtcacc cgctcggcgg cttctaatcc
      841 gtacttcaat atagcaatga gcagttaagc gtattactga aagttccaaa gagaaggttt
      901 ttttaggcta agataatggg gctctttaca gatctgttta gcttgcctcg tccccgccgg
      961 gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt gacgtgcgca
     1021 gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc catgtataat
     1081 catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac ggctcctcgc
     1141 tgcagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt ccccacgccg
     1201 cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc tttcatatac
     1261 ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat aaacaaccat
     1321 gggtaaggaa aagactcacg tttcgaggcc gcgattaaat tccaacatgg atgctgattt
     1381 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt
     1441 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     1501 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     1561 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     1621 caaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     1681 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     1741 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     1801 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     1861 taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     1921 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     1981 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     2041 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     2101 tcatttgatg ctcgatgagt ttttctaatc agtactgaca ataaaaagat tcttgttttc
     2161 aagaacttgt catttgtata gtttttttat attgtagttg ttctatttta atcaaatgtt
     2221 agcgtgattt atattttttt tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg
     2281 cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg tcgctatact gctgtcgatt
     2341 cgatactaac gccgccatcc agtttaaacg agctcgaatt catcgatgat atcagatcca
     2401 ctagtggcct atgcggccgc ggatctgccg gtctccctat agtgagtcgt attaatttcg
     2461 ataagccagg ttaacctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2521 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2581 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2641 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2701 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2761 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2821 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2881 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     2941 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3001 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3061 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3121 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     3181 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3241 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3301 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3361 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3421 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3481 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3541 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3601 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3661 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3721 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3781 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3841 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3901 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3961 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4021 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4081 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4141 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4201 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4261 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4321 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4381 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4441 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     4501 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     4561 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     4621 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     4681 gcggcatcag agcagattgt actgagagtg caccatatgg acatattgtc gttagaacgc
     4741 ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg acactata
//
