LOCUS       Exported                3503 bp DNA     circular SYN 14-JUL-2025
DEFINITION  Plasmid for yeast gene deletion using the bleMX6 selectable marker 
            conferring phleomycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Hentges P, Van Driessche B, Tafforeau L, Vandenhaute J, Carr AM.
  TITLE     Three novel antibiotic marker cassettes for gene disruption and 
            marker switching in Schizosaccharomyces pombe.
  JOURNAL   Yeast 2005;22:1013-9.
  PUBMED    16200533
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   EUROSCARF
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3503)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3503)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2005"; volume: "22"; pages: "1013-9"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            113..1034
                     /label=bleMX6
     promoter        113..456
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             457..831
                     /codon_start=1
                     /product="zeocin resistance protein"
                     /label=BleR
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      837..1034
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(1139..1157)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1415..2003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2177..3034)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3035..3139)
                     /label=AmpR promoter
     promoter        3485..3503
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 acgcggccgc cagctgaagc ttcgtacgct gcaggtcgac ggatccccgg gttaattaag
       61 gcgcgccaga tctgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga
      121 ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg
      181 tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga
      241 tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg agcagggaaa
      301 cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa
      361 aggttaggat ttgccactga ggttcttctt tcatatactt cctttttaaa tcttgctagg
      421 atacagttct cacatcacat ccgaacataa acaaccatgg ccaagttgac cagtgccgtt
      481 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
      541 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
      601 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
      661 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
      721 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
      781 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg atcagtactg
      841 acaataaaaa gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag
      901 ttgttctatt ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct
      961 gcccagatgc gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc
     1021 tggtcgctat actgctgtcg attcgatact aacgccgcca tccagtttaa acgagctcga
     1081 attcatcgat gatatcagat ccactagtgg cctatgcggc cgcggatctg ccggtctccc
     1141 tatagtgagt cgtattaatt tcgataagcc aggttaacct gcattaatga atcggccaac
     1201 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1261 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1321 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1381 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1441 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1501 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1561 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
     1621 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1681 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1741 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1801 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     1861 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1921 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1981 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2041 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2101 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2161 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     2221 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     2281 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     2341 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     2401 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     2461 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     2521 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     2581 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     2641 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     2701 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     2761 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     2821 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     2881 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     2941 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     3001 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     3061 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     3121 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     3181 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     3241 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     3301 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     3361 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     3421 tggacatatt gtcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca
     3481 tacgatttag gtgacactat aga
//
