LOCUS       40924_19636        4474 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast tagging vector pFA6a-VC-kanMX6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Sung MK, Huh WK.
  TITLE     Bimolecular fluorescence complementation analysis system for in vivo
            detection of protein-protein interaction in Saccharomyces cerevisiae
  JOURNAL   Yeast 24 (9), 767-775 (2007)
  PUBMED    17534848
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Sung M-K., Huh W-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2007) School of Biological Sciences, Seoul 
            National University, Seoul 151-747, Republic of Korea
REFERENCE   3  (bases 1 to 4474)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4474)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2007"; volume: "24"; issue: "9"; pages: "767-775"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-JAN-2007) School of Biological Sciences, Seoul National 
            University, Seoul 151-747, Republic of Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             114..365
                     /codon_start=1
                     /label=VC155
                     /note="C-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
                     /translation="DKQKNGIKLNFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     terminator      389..576
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     gene            651..2007
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     promoter        complement(2112..2130)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2388..2976)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3150..4007)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4008..4112)
                     /label=AmpR promoter
     promoter        4458..4474
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 accgtccggc gtgcaaaatc ccgaacgacc tgaaacagaa agtcatgaac cacgacaagc
      121 agaagaacgg catcaagctg aacttcaaga tccgccacaa catcgaggac ggcggcgtgc
      181 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      241 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
      301 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      361 acaagtaggg cgcgccactt ctaaataagc gaatttctta tgatttatga tttttattat
      421 taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa
      481 acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata
      541 gtatgaggtc gctcttattg accacacctc taccggcaga tccgctaggg ataacagggt
      601 aatatagatc tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg
      661 cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc
      721 gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata cattttgatg
      781 gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg
      841 ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag
      901 gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat
      961 acagttctca catcacatcc gaacataaac aaccatgggt aaggaaaaga ctcacgtttc
     1021 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     1081 taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga
     1141 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     1201 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     1261 tgatgatgca tggttactca ccactgcgat ccccggcaaa acagcattcc aggtattaga
     1321 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     1381 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     1441 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     1501 tggctggcct gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga
     1561 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     1621 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     1681 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     1741 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     1801 ctaatcagta ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt
     1861 ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc
     1921 ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat
     1981 cgtatgtgaa tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtt
     2041 taaacgagct cgaattcatc gatgatatca gatccactag tggcctatgc ggccgcggat
     2101 ctgccggtct ccctatagtg agtcgtatta atttcgataa gccaggttaa cctgcattaa
     2161 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2221 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2281 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2341 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2401 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2461 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2521 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2581 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2641 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2701 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2761 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2821 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2881 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2941 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3001 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3061 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3121 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3181 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3241 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3301 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3361 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3421 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3481 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3541 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3601 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3661 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3721 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3781 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3841 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3901 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3961 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4021 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4081 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     4141 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     4201 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     4261 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     4321 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     4381 agagtgcacc atatggacat attgtcgtta gaacgcggct acaattaata cataacctta
     4441 tgtatcatac acatacgatt taggtgacac tata
//
