LOCUS       40924_19631        4318 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast tagging vector pFA6a-VC-His3MX6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Sung MK, Huh WK.
  TITLE     Bimolecular fluorescence complementation analysis system for in vivo
            detection of protein-protein interaction in Saccharomyces cerevisiae
  JOURNAL   Yeast 24 (9), 767-775 (2007)
  PUBMED    17534848
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Sung M-K., Huh W-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2007) School of Biological Sciences, Seoul 
            National University, Seoul 151-747, Republic of Korea
REFERENCE   3  (bases 1 to 4318)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4318)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2007"; volume: "24"; issue: "9"; pages: "767-775"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-JAN-2007) School of Biological Sciences, Seoul National 
            University, Seoul 151-747, Republic of Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             114..365
                     /codon_start=1
                     /label=VC155
                     /note="C-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
                     /translation="DKQKNGIKLNFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     terminator      389..576
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     gene            651..1851
                     /label=HIS3MX6
                     /note="yeast selectable marker encoding the S. pombe his5
                     gene, which corresponds to S. cerevisiae HIS3"
     promoter        complement(1956..1974)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2232..2820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2994..3851)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3852..3956)
                     /label=AmpR promoter
     promoter        4302..4318
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 accgtccggc gtgcaaaatc ccgaacgacc tgaaacagaa agtcatgaac cacgacaagc
      121 agaagaacgg catcaagctg aacttcaaga tccgccacaa catcgaggac ggcggcgtgc
      181 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      241 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
      301 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      361 acaagtaggg cgcgccactt ctaaataagc gaatttctta tgatttatga tttttattat
      421 taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa
      481 acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata
      541 gtatgaggtc gctcttattg accacacctc taccggcaga tccgctaggg ataacagggt
      601 aatatagatc tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg
      661 cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc
      721 gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata cattttgatg
      781 gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg
      841 ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag
      901 gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat
      961 acagttctca catcacatcc gaacataaac aaccatgggt aggagggctt ttgtagaaag
     1021 aaatacgaac gaaacgaaaa tcagcgttgc catcgctttg gacaaagctc ccttacctga
     1081 agagtcgaat tttattgatg aacttataac ttccaagcat gcaaaccaaa agggagaaca
     1141 agtaatccaa gtagacacgg gaattggatt cttggatcac atgtatcatg cactggctaa
     1201 acatgcaggc tggagcttac gactttactc aagaggtgat ttaatcatcg atgatcatca
     1261 cactgcagaa gatactgcta ttgcacttgg tattgcattc aagcaggcta tgggtaactt
     1321 tgccggcgtt aaaagatttg gacatgctta ttgtccactt gacgaagctc tttctagaag
     1381 cgtagttgac ttgtcgggac ggccctatgc tgttatcgat ttgggattaa agcgtgaaaa
     1441 ggttggggaa ttgtcctgtg aaatgatccc tcacttacta tattcctttt cggtagcagc
     1501 tggaattact ttgcatgtta cctgcttata tggtagtaat gaccatcatc gtgctgaaag
     1561 cgcttttaaa tctctggctg ttgccatgcg cgcggctact agtcttactg gaagttctga
     1621 agtcccaagc acgaagggag tgttgtaaag agtactgaca ataaaaagat tcttgttttc
     1681 aagaacttgt catttgtata gtttttttat attgtagttg ttctatttta atcaaatgtt
     1741 agcgtgattt atattttttt tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg
     1801 cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg tcgctatact gctgtcgatt
     1861 cgatactaac gccgccatcc agtttaaacg agctcgaatt catcgatgat atcagatcca
     1921 ctagtggcct atgcggccgc ggatctgccg gtctccctat agtgagtcgt attaatttcg
     1981 ataagccagg ttaacctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2041 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2101 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2161 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2221 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2281 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2341 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2401 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     2461 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2521 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2581 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2641 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     2701 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2761 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2821 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2881 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2941 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3001 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3061 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3121 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3181 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3241 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3301 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3361 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3421 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3481 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3541 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3601 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3661 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3721 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3781 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3841 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3901 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3961 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     4021 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     4081 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     4141 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     4201 gcggcatcag agcagattgt actgagagtg caccatatgg acatattgtc gttagaacgc
     4261 ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg acactata
//
