LOCUS       62056_10710        4904 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        132..475
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1012..1803
                     /codon_start=1
                     /label=S. pombe ura4
                     /note="orotidine 5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     terminator      2254..2451
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(2559..2577)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2835..3423)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3597..4454)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4455..4559)
                     /label=AmpR promoter
ORIGIN
        1 atttaggtga cactatagaa cgcggccgcc agctgaagct tcgtacgctg caggtcgacg
       61 gatccccggg ttaattaagg cgcgccagat ctgtttagct tgcctcgtcc ccgccgggtc
      121 acccggccag cgacatggag gcccagaata ccctccttga cagtcttgac gtgcgcagct
      181 caggggcatg atgtgactgt cgcccgtaca tttagcccat acatccccat gtataatcat
      241 ttgcatccat acattttgat ggccgcacgg cgcgaagcaa aaattacggc tcctcgctgc
      301 agacctgcga gcagggaaac gctcccctca cagacgcgtt gaattgtccc cacgccgcgc
      361 ccctgtagag aaatataaaa ggttaggatt tgccactgag gttcttcttt catatacttc
      421 cttttaaaat cttgctagga tacagttctc acatcacatc cgaacataaa caaccatggc
      481 tagaagctac aaatcccact ggctatatgt atgcatttgt gttaaaaaag tttgtataga
      541 ttatttaatc tactcagcat tctttctcta aataggaatt tgttacttaa tggagaaaaa
      601 aatgtttcga tttacctagt gtatttgttt gtatactcac gtttaatttc aaacatccat
      661 tctatcttgt gtaatttttg gcatggtgaa aaagataatc agccttataa tctttacaaa
      721 agtaagaaat tctgtaaata agccttaatg cccttgcttt aaattaaaat ggttcttttt
      781 catgataatg tttgcacttt gtgaatatat tttagatagt tctgtgaggt ataattaaga
      841 tgttttagag acttatacaa ttttgtcttt ataaattctt aattgatttt accatcccag
      901 tttaactatg cttcgtcggc atctctgcac atgtcgtgtt ttcttaccgt attgtcctac
      961 caagaacctc ttttttgctt ggatcgaaat taaaggttta aaagcaaagt tatggatgct
     1021 agagtatttc aaagctattc agctagagct gaggggatga aaaatcccat tgccaaggaa
     1081 ttgttggctt tgatggaaga aaagcaaagc aacttgtcag tcgcggtcga tttgacgaag
     1141 aaatccgaaa tcttagaatt ggtagataaa attggaccct atgtctgtgt tatcaagaca
     1201 catattgacg ttgtcgagga tttcgaccag gatatggtag aaaaactggt ggccttaggt
     1261 aaaaagcatc gttttcttat ctttgaggat cgcaaattcg cagacattgg aaataccgtc
     1321 aagctacaat atgcatctgg tgtgtacaaa attgcttctt gggctcatat cacaaattgc
     1381 catacagtgc caggcgaggg tattatacaa ggcctcaaag aagttggttt acctttggga
     1441 cgtggtctct tgcttttggc tgaaatgtct tccaaaggct ctttggctac tggttcctac
     1501 acagagaaaa ccttagaatg gtttgagaag cataccgatt tttgctttgg ctttatagct
     1561 ggtcgtcgat ttcctaacct tcaaagcgac tacataacta tgtcccctgg tatcggcttg
     1621 gatgttaaag gagacgggct gggacagcaa tatcgtactc ctgaagaagt gattgtaaac
     1681 tgcggtagcg atatcatcat tgttggtcgt ggagtctatg gagctggtcg taatcctgtt
     1741 gtcgaagcca agagatatag agaagctggt tggaaggcat atcagcaaag actttctcag
     1801 cattaaaaaa agactaatgt aaaatttttt tggttggtta ttgaaaaagt cgatgccttg
     1861 tttgcgtttg ttttcctagg cgttttatgt cagaaggcat ttagaattag tatacaagta
     1921 ctctttggta aaattttatg tagcgactaa aatattaact attatagata aacaccttgg
     1981 gaataaaaag taatttgcta tagtaattta ttaaacatgc tcctacaaca ttaccacaat
     2041 cttttctctt ggattgacat tgaataagaa aagagtgaat ttttttagac ttgtaatgat
     2101 aactatgtac aaagccaatg aaagatgtat gtagatgaat gtaaaatacc atgtagacaa
     2161 acaagataaa acttggttat aaacattggt gttggaacag aataaattag atgtcaaaaa
     2221 gtttcgtcaa tatcactcta gcccctaatc agtactgaca ataaaaagat tcttgttttc
     2281 aagaacttgt catttgtata gtttttttat attgtagttg ttctatttta atcaaatgtt
     2341 agcgtgattt atattttttt tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg
     2401 cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg tcgctatact gctgtcgatt
     2461 cgatactaac gccgccatcc agtgtcgaaa acgagctcga attcatcgat gatatcagat
     2521 ccactagtgg cctatgcggc cgcggatctg ccggtctccc tatagtgagt cgtattaatt
     2581 tcgataagcc aggttaacct gcattaatga atcggccaac gcgcggggag aggcggtttg
     2641 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     2701 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2761 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2821 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2881 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2941 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3001 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3061 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3121 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3181 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3241 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3301 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3361 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3421 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3481 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3541 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     3601 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3661 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3721 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3781 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3841 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3901 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3961 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     4021 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     4081 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     4141 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     4201 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4261 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4321 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     4381 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     4441 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     4501 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     4561 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     4621 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     4681 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     4741 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     4801 tatgcggcat cagagcagat tgtactgaga gtgcaccata tggacatatt gtcgttagaa
     4861 cgcggctaca attaatacat aaccttatgt atcatacaca tacg
//
