LOCUS       40924_19626        5104 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast tagging vector pFA6a-URA3-kanMX6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Iwase M, Nishihama R, Vallen EA, Luo J, Fang X, Tong Z, Guttman J, 
            Meisel R, Hanna J, Bi E.
  TITLE     A URA3-Kan cassette: diverse gene modifications in Saccharomyces 
            cerevisiae without leaving a trace
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Nishihama R.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2010) Department of Genetics, Stanford University 
            School of Medicine, 300 Pasteur Drive, Stanford, CA 94305-5120, USA
REFERENCE   3  (bases 1 to 5104)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5104)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2010) Department of Genetics, Stanford University School of 
            Medicine, 300 Pasteur Drive, Stanford, CA 94305-5120, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        113..333
                     /label=URA3 promoter
     CDS             334..1134
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     gene            1281..2637
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     promoter        complement(2742..2760)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3018..3606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3780..4637)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4638..4742)
                     /label=AmpR promoter
     promoter        5088..5104
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 aggcgcgcct atgcggcatc agagcagatt gtactgagag tgcaccacgc ttttcaattc
      121 aattcatcat ttttttttta ttcttttttt tgatttcggt ttctttgaaa tttttttgat
      181 tcggtaatct ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat
      241 atacgcatat gtagtgttga agaaacatga aattgcccag tattcttaac ccaactgcac
      301 agaacaaaaa cctgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt
      361 gctgctactc atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa
      421 acaaacttgt gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa
      481 gcattaggtc ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc
      541 atggagggca cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc
      601 gaagacagaa aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta
      661 tacagaatag cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt
      721 gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg ccttttgatg
      781 ttagcagaat tgtcatgcaa gggctcccta tctactggag aatatactaa gggtactgtt
      841 gacattgcga agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt
      901 ggaagagatg aaggttacga ttggttgatt atgacacccg gtgtgggttt agatgacaag
      961 ggagacgcat tgggtcaaca gtatagaacc gtggatgatg tggtctctac aggatctgac
     1021 attattattg ttggaagagg actatttgca aagggaaggg atgctaaggt agagggtgaa
     1081 cgttacagaa aagcaggctg ggaagcatat ttgagaagat gcggccagca aaactaaaaa
     1141 actgtattat aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta
     1201 tatcagttat taccctgcgg tgtgaaatac cgcacagatc tgtttagctt gcctcgtccc
     1261 cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac agtcttgacg
     1321 tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata catccccatg
     1381 tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa aattacggct
     1441 cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg aattgtcccc
     1501 acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg ttcttctttc
     1561 atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc gaacataaac
     1621 aaccatgggt aaggaaaaga ctcacgtttc gaggccgcga ttaaattcca acatggatgc
     1681 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     1741 tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     1801 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     1861 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     1921 ccccggcaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     1981 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     2041 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     2101 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     2161 aatgcataag cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     2221 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     2281 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     2341 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     2401 gcagtttcat ttgatgctcg atgagttttt ctaatcagta ctgacaataa aaagattctt
     2461 gttttcaaga acttgtcatt tgtatagttt ttttatattg tagttgttct attttaatca
     2521 aatgttagcg tgatttatat tttttttcgc ctcgacatca tctgcccaga tgcgaagtta
     2581 agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg
     2641 tcgattcgat actaacgccg ccatccagtt taaacgagct cgaattcatc gatgatatca
     2701 gatccactag tggcctatgc ggccgcggat ctgccggtct ccctatagtg agtcgtatta
     2761 atttcgataa gccaggttaa cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     2821 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2881 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2941 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3001 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3061 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3121 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3181 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     3241 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3301 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3361 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3421 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     3481 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3541 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3601 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3661 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3721 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3781 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3841 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3901 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3961 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4021 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4081 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4141 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4201 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4261 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4321 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4381 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4441 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4501 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     4561 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     4621 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     4681 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     4741 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     4801 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     4861 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     4921 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     4981 aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat attgtcgtta
     5041 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
     5101 tata
//
