LOCUS       40924_19621        3415 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid for yeast gene deletion using the S. cerevisiae TRP1 
            selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 3415)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3415)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        76..223
                     /label=TRP1 promoter
     CDS             224..895
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(1053..1071)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1329..1917)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2091..2948)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2949..3053)
                     /label=AmpR promoter
     promoter        3399..3415
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 aggcgcgcca gatctgtaca atcttgatcc ggagcttttc tttttttgcc gattaagaat
      121 taattcggtc gaaaaaagaa aaggagaggg ccaagaggga gggcattggt gactattgag
      181 cacgtgagta tacgtgatta agcacacaaa ggcagcttgg agtatgtctg ttattaattt
      241 cacaggtagt tctggtccat tggtgaaagt ttgcggcttg cagagcacag aggccgcaga
      301 atgtgctcta gattccgatg ctgacttgct gggtattata tgtgtgccca atagaaagag
      361 aacaattgac ccggttattg caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag
      421 ttcaggcact ccgaaatact tggttggcgt gtttcgtaat caacctaagg aggatgtttt
      481 ggctctggtc aatgattacg gcattgatat cgtccaactg catggagatg agtcgtggca
      541 agaataccaa gagttcctcg gtttgccagt tattaaaaga ctcgtatttc caaaagactg
      601 caacatacta ctcagtgcag cttcacagaa acctcattcg tttattccct tgtttgattc
      661 agaagcaggt gggacaggtg aacttttgga ttggaactcg atttctgact gggttggaag
      721 gcaagagagc cccgaaagct tacattttat gttagctggt ggactgacgc cagaaaatgt
      781 tggtgatgcg cttagattaa atggcgttat tggtgttgat gtaagcggag gtgtggagac
      841 aaatggtgta aaagactcta acaaaatagc aaatttcgtc aaaaatgcta agaaataggt
      901 tattactgag tagtatttat ttaagtattg tttgtgcact tgcctgcggt gtgaaatacc
      961 gcacagatgc gtaaggaggt ttaaacgagc tcgaattcat cgatgatatc agatccacta
     1021 gtggcctatg cggccgcgga tctgccggtc tccctatagt gagtcgtatt aatttcgata
     1081 agccaggtta acctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     1141 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1201 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1261 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1321 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1381 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1441 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1501 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     1561 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1621 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1681 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1741 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     1801 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1861 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1921 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1981 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2041 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2101 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2161 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2221 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2281 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2341 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2401 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2461 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2521 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2581 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2641 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2701 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2761 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2821 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2881 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2941 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3001 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3061 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     3121 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     3181 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     3241 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     3301 gcatcagagc agattgtact gagagtgcac catatggaca tattgtcgtt agaacgcggc
     3361 tacaattaat acataacctt atgtatcata cacatacgat ttaggtgaca ctata
//
