LOCUS       40924_19606        3962 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid for swapping in the GAL1 promoter using the TRP1 selectable 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 3962)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3962)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(87..528)
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     promoter        623..770
                     /label=TRP1 promoter
     CDS             771..1442
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(1600..1618)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1876..2464)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2638..3495)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3496..3600)
                     /label=AmpR promoter
     promoter        3946..3962
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 aagacatttt gagatccggg ttttttctcc ttgacgttaa agtatagagg tatattaaca
      121 attttttgtt gatactttta ttacatttga ataagaagta atacaaaccg aaaatgttga
      181 aagtattagt taaagtggtt atgcagtttt tgcatttata tatctgttaa tagatcaaaa
      241 atcatcgctt cgctgattaa ttaccccaga aataaggcta aaaaactaat cgcattatca
      301 tcctatggtt gttaatttga ttcgttcatt tgaaggtttg tggggccagg ttactgccaa
      361 tttttcctct tcataaccat aaaagctagt attgtagaat ctttattgtt cggagcagtg
      421 cggcgcgagg cacatctgcg tttcaggaac gcgaccggtg aagacgagga cgcacggagg
      481 agagtcttcc ttcggagggc tgtcacccgc tcggcggctt ctaatccgta cttcaatata
      541 gcaatgagca gttaagcgta ttactgaaag ttccaaagag aaggtttttt taggctaaga
      601 taatggggct ctttacagat ctgtacaatc ttgatccgga gcttttcttt ttttgccgat
      661 taagaattaa ttcggtcgaa aaaagaaaag gagagggcca agagggaggg cattggtgac
      721 tattgagcac gtgagtatac gtgattaagc acacaaaggc agcttggagt atgtctgtta
      781 ttaatttcac aggtagttct ggtccattgg tgaaagtttg cggcttgcag agcacagagg
      841 ccgcagaatg tgctctagat tccgatgctg acttgctggg tattatatgt gtgcccaata
      901 gaaagagaac aattgacccg gttattgcaa ggaaaatttc aagtcttgta aaagcatata
      961 aaaatagttc aggcactccg aaatacttgg ttggcgtgtt tcgtaatcaa cctaaggagg
     1021 atgttttggc tctggtcaat gattacggca ttgatatcgt ccaactgcat ggagatgagt
     1081 cgtggcaaga ataccaagag ttcctcggtt tgccagttat taaaagactc gtatttccaa
     1141 aagactgcaa catactactc agtgcagctt cacagaaacc tcattcgttt attcccttgt
     1201 ttgattcaga agcaggtggg acaggtgaac ttttggattg gaactcgatt tctgactggg
     1261 ttggaaggca agagagcccc gaaagcttac attttatgtt agctggtgga ctgacgccag
     1321 aaaatgttgg tgatgcgctt agattaaatg gcgttattgg tgttgatgta agcggaggtg
     1381 tggagacaaa tggtgtaaaa gactctaaca aaatagcaaa tttcgtcaaa aatgctaaga
     1441 aataggttat tactgagtag tatttattta agtattgttt gtgcacttgc ctgcggtgtg
     1501 aaataccgca cagatgcgta aggaggttta aacgagctcg aattcatcga tgatatcaga
     1561 tccactagtg gcctatgcgg ccgcggatct gccggtctcc ctatagtgag tcgtattaat
     1621 ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     1681 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     1741 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     1801 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1861 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     1921 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1981 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2041 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     2101 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2161 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2221 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2281 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     2341 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2401 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2461 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     2521 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     2581 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     2641 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     2701 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     2761 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     2821 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     2881 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     2941 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3001 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     3061 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     3121 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     3181 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     3241 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     3301 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     3361 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     3421 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     3481 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     3541 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     3601 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     3661 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     3721 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     3781 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     3841 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat tgtcgttaga
     3901 acgcggctac aattaataca taaccttatg tatcatacac atacgattta ggtgacacta
     3961 ta
//
