LOCUS       pFA6a-TRP1-PGAL1        4842 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid with a TRP1 marker for swapping in the GAL1 promoter and 
            adding a GST tag.
ACCESSION   .
VERSION     .
KEYWORDS    pFA6a-TRP1-PGAL1-GST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 4842)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4842)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(50..237)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(279..932)
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             complement(945..947)
                     /codon_start=1
                     /label=start codon
                     /note="start codon"
                     /translation="M"
     promoter        complement(967..1408)
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     promoter        1503..1650
                     /label=TRP1 promoter
     CDS             1651..2322
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
     promoter        complement(2480..2498)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2756..3344)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3518..4375)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4376..4480)
                     /label=AmpR promoter
     promoter        4826..4842
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatctgc cggtagaggt
       61 gtggtcaata agagcgacct catgctatac ctgagaaagc aacctgacct acaggaaaga
      121 gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa atttgtatac
      181 acttattttt tttataactt atttaataat aaaaatcata aatcataaga aattcgctta
      241 tttagaagtg gcgcgcctca acgcggaacc agatccgatt ttggaggatg gtcgccacca
      301 ccaaacgtgg cttgccagcc ctgcaaaggc catgctatat acttgctgga tttcaagtac
      361 ttatcaattt gtgggatagc ttcaatacgt tttttaaaac aaactaattt tgggaacgca
      421 tccaggcaca ttgggtccat gtataaaaca acatcaagag cgtcatacaa catgaagtca
      481 ggatgggtta catgatcacc atttaaatat gttttatgac ataaacgatc ttcgaacatt
      541 ttcagcattt caggtagctt gctaagaaaa tcaactttga gagtttcaaa gtctttacta
      601 tatgcaattc tcgaaacacc gtatctaata tccaaaaccg ctccttcaag cattgaaatc
      661 tctgcacgct cttttggaca accacccaac atgttgtgct tgtcagctat ataacgtatg
      721 atggccatag actgtgttaa tttaacatca ccatcaatat aataaggaag attgggaaac
      781 tccaaaccca attcaaactt tttgtttcgc catttatcac cttcatcgcg ctcatacaaa
      841 tgctcttcat atttttcttc aagatattcc aaaagaagtc gagtgggttg cacaaggccc
      901 ttaattttcc aataacctag tataggggac atattaatta aagacatttt gagatccggg
      961 ttttttctcc ttgacgttaa agtatagagg tatattaaca attttttgtt gatactttta
     1021 ttacatttga ataagaagta atacaaaccg aaaatgttga aagtattagt taaagtggtt
     1081 atgcagtttt tgcatttata tatctgttaa tagatcaaaa atcatcgctt cgctgattaa
     1141 ttaccccaga aataaggcta aaaaactaat cgcattatca tcctatggtt gttaatttga
     1201 ttcgttcatt tgaaggtttg tggggccagg ttactgccaa tttttcctct tcataaccat
     1261 aaaagctagt attgtagaat ctttattgtt cggagcagtg cggcgcgagg cacatctgcg
     1321 tttcaggaac gcgaccggtg aagacgagga cgcacggagg agagtcttcc ttcggagggc
     1381 tgtcacccgc tcggcggctt ctaatccgta cttcaatata gcaatgagca gttaagcgta
     1441 ttactgaaag ttccaaagag aaggtttttt taggctaaga taatggggct ctttacagat
     1501 ctgtacaatc ttgatccgga gcttttcttt ttttgccgat taagaattaa ttcggtcgaa
     1561 aaaagaaaag gagagggcca agagggaggg cattggtgac tattgagcac gtgagtatac
     1621 gtgattaagc acacaaaggc agcttggagt atgtctgtta ttaatttcac aggtagttct
     1681 ggtccattgg tgaaagtttg cggcttgcag agcacagagg ccgcagaatg tgctctagat
     1741 tccgatgctg acttgctggg tattatatgt gtgcccaata gaaagagaac aattgacccg
     1801 gttattgcaa ggaaaatttc aagtcttgta aaagcatata aaaatagttc aggcactccg
     1861 aaatacttgg ttggcgtgtt tcgtaatcaa cctaaggagg atgttttggc tctggtcaat
     1921 gattacggca ttgatatcgt ccaactgcat ggagatgagt cgtggcaaga ataccaagag
     1981 ttcctcggtt tgccagttat taaaagactc gtatttccaa aagactgcaa catactactc
     2041 agtgcagctt cacagaaacc tcattcgttt attcccttgt ttgattcaga agcaggtggg
     2101 acaggtgaac ttttggattg gaactcgatt tctgactggg ttggaaggca agagagcccc
     2161 gaaagcttac attttatgtt agctggtgga ctgacgccag aaaatgttgg tgatgcgctt
     2221 agattaaatg gcgttattgg tgttgatgta agcggaggtg tggagacaaa tggtgtaaaa
     2281 gactctaaca aaatagcaaa tttcgtcaaa aatgctaaga aataggttat tactgagtag
     2341 tatttattta agtattgttt gtgcacttgc ctgcggtgtg aaataccgca cagatgcgta
     2401 aggaggttta aacgagctcg aattcatcga tgatatcaga tccactagtg gcctatgcgg
     2461 ccgcggatct gccggtctcc ctatagtgag tcgtattaat ttcgataagc caggttaacc
     2521 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     2581 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     2641 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2701 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2761 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2821 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2881 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2941 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3001 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3061 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3121 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3181 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3241 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3301 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3361 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     3421 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3481 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3541 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3601 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     3661 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     3721 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     3781 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     3841 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     3901 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     3961 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     4021 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     4081 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     4141 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     4201 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     4261 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     4321 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     4381 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     4441 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     4501 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     4561 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     4621 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     4681 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     4741 ttgtactgag agtgcaccat atggacatat tgtcgttaga acgcggctac aattaataca
     4801 taaccttatg tatcatacac atacgattta ggtgacacta ta
//
