LOCUS       pFA6a-TRP1-PGAL1        4275 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid with a TRP1 marker for swapping in the GAL1 promoter and 
            adding a triple-HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    pFA6a-TRP1-PGAL1-3HA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 4275)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4275)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(50..237)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(270..359)
                     /label=3xHA
                     /note="three tandem HA epitope tags"
     CDS             complement(378..380)
                     /codon_start=1
                     /label=start codon
                     /note="start codon"
                     /translation="M"
     promoter        complement(400..841)
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     promoter        936..1083
                     /label=TRP1 promoter
     CDS             1084..1755
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
     promoter        complement(1913..1931)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2189..2777)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2951..3808)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3809..3913)
                     /label=AmpR promoter
     promoter        4259..4275
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatctgc cggtagaggt
       61 gtggtcaata agagcgacct catgctatac ctgagaaagc aacctgacct acaggaaaga
      121 gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa atttgtatac
      181 acttattttt tttataactt atttaataat aaaaatcata aatcataaga aattcgctta
      241 tttagaagtg gcgcgcctca gcactgagca gcgtaatctg gaacgtcata tggataggat
      301 cctgcatagt ccgggacgtc atacggatag cccgcatagt caggaacatc gtatgggtaa
      361 aagatgttaa ttaaagacat tttgagatcc gggttttttc tccttgacgt taaagtatag
      421 aggtatatta acaatttttt gttgatactt ttattacatt tgaataagaa gtaatacaaa
      481 ccgaaaatgt tgaaagtatt agttaaagtg gttatgcagt ttttgcattt atatatctgt
      541 taatagatca aaaatcatcg cttcgctgat taattacccc agaaataagg ctaaaaaact
      601 aatcgcatta tcatcctatg gttgttaatt tgattcgttc atttgaaggt ttgtggggcc
      661 aggttactgc caatttttcc tcttcataac cataaaagct agtattgtag aatctttatt
      721 gttcggagca gtgcggcgcg aggcacatct gcgtttcagg aacgcgaccg gtgaagacga
      781 ggacgcacgg aggagagtct tccttcggag ggctgtcacc cgctcggcgg cttctaatcc
      841 gtacttcaat atagcaatga gcagttaagc gtattactga aagttccaaa gagaaggttt
      901 ttttaggcta agataatggg gctctttaca gatctgtaca atcttgatcc ggagcttttc
      961 tttttttgcc gattaagaat taattcggtc gaaaaaagaa aaggagaggg ccaagaggga
     1021 gggcattggt gactattgag cacgtgagta tacgtgatta agcacacaaa ggcagcttgg
     1081 agtatgtctg ttattaattt cacaggtagt tctggtccat tggtgaaagt ttgcggcttg
     1141 cagagcacag aggccgcaga atgtgctcta gattccgatg ctgacttgct gggtattata
     1201 tgtgtgccca atagaaagag aacaattgac ccggttattg caaggaaaat ttcaagtctt
     1261 gtaaaagcat ataaaaatag ttcaggcact ccgaaatact tggttggcgt gtttcgtaat
     1321 caacctaagg aggatgtttt ggctctggtc aatgattacg gcattgatat cgtccaactg
     1381 catggagatg agtcgtggca agaataccaa gagttcctcg gtttgccagt tattaaaaga
     1441 ctcgtatttc caaaagactg caacatacta ctcagtgcag cttcacagaa acctcattcg
     1501 tttattccct tgtttgattc agaagcaggt gggacaggtg aacttttgga ttggaactcg
     1561 atttctgact gggttggaag gcaagagagc cccgaaagct tacattttat gttagctggt
     1621 ggactgacgc cagaaaatgt tggtgatgcg cttagattaa atggcgttat tggtgttgat
     1681 gtaagcggag gtgtggagac aaatggtgta aaagactcta acaaaatagc aaatttcgtc
     1741 aaaaatgcta agaaataggt tattactgag tagtatttat ttaagtattg tttgtgcact
     1801 tgcctgcggt gtgaaatacc gcacagatgc gtaaggaggt ttaaacgagc tcgaattcat
     1861 cgatgatatc agatccacta gtggcctatg cggccgcgga tctgccggtc tccctatagt
     1921 gagtcgtatt aatttcgata agccaggtta acctgcatta atgaatcggc caacgcgcgg
     1981 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2041 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2101 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2161 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2221 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2281 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2341 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
     2401 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2461 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2521 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2581 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     2641 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2701 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2761 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2821 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2881 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2941 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3001 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3061 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3121 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3181 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3241 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3301 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3361 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3421 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3481 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3541 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3601 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3661 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3721 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3781 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3841 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3901 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     3961 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     4021 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     4081 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     4141 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatggaca
     4201 tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat
     4261 ttaggtgaca ctata
//
