LOCUS       40924_19346        5148 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast tagging vector pFA6a-His3MX6-pCET1-VN, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Sung MK, Huh WK.
  TITLE     Bimolecular fluorescence complementation analysis system for in vivo
            detection of protein-protein interaction in Saccharomyces cerevisiae
  JOURNAL   Yeast 24 (9), 767-775 (2007)
  PUBMED    17534848
REFERENCE   2  (bases 1 to 5148)
  AUTHORS   Sung M-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2007) School of Biological Sciences, Seoul 
            National University, Seoul 151-747, Republic of Korea
REFERENCE   3  (bases 1 to 5148)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5148)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2007"; volume: "24"; issue: "9"; pages: "767-775"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-MAR-2007) School of Biological Sciences, Seoul National 
            University, Seoul 151-747, Republic of Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..19)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        365..469
                     /label=AmpR promoter
     CDS             470..1327
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1501..2089
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2347..2365
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            complement(2470..3670)
                     /label=HIS3MX6
                     /note="yeast selectable marker encoding the S. pombe his5
                     gene, which corresponds to S. cerevisiae HIS3"
     CDS             4344..4862
                     /codon_start=1
                     /label=VN173
                     /note="N-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     terminator      4886..5073
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
ORIGIN
        1 tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc
       61 gcgttctaac gacaatatgt ccatatggtg cactctcagt acaatctgct ctgatgccgc
      121 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
      181 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
      241 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
      301 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
      361 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
      421 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
      481 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
      541 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
      601 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
      661 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
      721 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
      781 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
      841 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
      901 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
      961 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     1021 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     1081 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     1141 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     1201 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     1261 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     1321 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     1381 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     1441 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1501 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1561 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     1621 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     1681 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1741 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1801 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     1861 ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg
     1921 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     1981 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2041 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2101 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     2161 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     2221 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     2281 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaggttaa cctggcttat
     2341 cgaaattaat acgactcact atagggagac cggcagatcc gcggccgcat aggccactag
     2401 tggatctgat atcatcgatg aattcgagct cgtttaaact ggatggcggc gttagtatcg
     2461 aatcgacagc agtatagcga ccagcattca catacgattg acgcatgata ttactttctg
     2521 cgcacttaac ttcgcatctg ggcagatgat gtcgaggcga aaaaaaatat aaatcacgct
     2581 aacatttgat taaaatagaa caactacaat ataaaaaaac tatacaaatg acaagttctt
     2641 gaaaacaaga atctttttat tgtcagtact ctttacaaca ctcccttcgt gcttgggact
     2701 tcagaacttc cagtaagact agtagccgcg cgcatggcaa cagccagaga tttaaaagcg
     2761 ctttcagcac gatgatggtc attactacca tataagcagg taacatgcaa agtaattcca
     2821 gctgctaccg aaaaggaata tagtaagtga gggatcattt cacaggacaa ttccccaacc
     2881 ttttcacgct ttaatcccaa atcgataaca gcatagggcc gtcccgacaa gtcaactacg
     2941 cttctagaaa gagcttcgtc aagtggacaa taagcatgtc caaatctttt aacgccggca
     3001 aagttaccca tagcctgctt gaatgcaata ccaagtgcaa tagcagtatc ttctgcagtg
     3061 tgatgatcat cgatgattaa atcacctctt gagtaaagtc gtaagctcca gcctgcatgt
     3121 ttagccagtg catgatacat gtgatccaag aatccaattc ccgtgtctac ttggattact
     3181 tgttctccct tttggtttgc atgcttggaa gttataagtt catcaataaa attcgactct
     3241 tcaggtaagg gagctttgtc caaagcgatg gcaacgctga ttttcgtttc gttcgtattt
     3301 ctttctacaa aagccctcct acccatggtt gtttatgttc ggatgtgatg tgagaactgt
     3361 atcctagcaa gattttaaaa ggaagtatat gaaagaagaa cctcagtggc aaatcctaac
     3421 cttttatatt tctctacagg ggcgcggcgt ggggacaatt caacgcgtct gtgaggggag
     3481 cgtttccctg ctcgcaggtc tgcagcgagg agccgtaatt tttgcttcgc gccgtgcggc
     3541 catcaaaatg tatggatgca aatgattata catggggatg tatgggctaa atgtacgggc
     3601 gacagtcaca tcatgcccct gagctgcgca cgtcaagact gtcaaggagg gtattctggg
     3661 cctccatgtc gctggccggg tgacccggcg gggacgaggc aagctaaaca gatccatgtc
     3721 aagccgtttc atttcctact tatttttaat tatcggtttc ttctattcat gtagttcact
     3781 attcaattag tatatgtaga taaaaggaga agggttttat ctgcaaacag acgcaagacg
     3841 tatgtactaa gcactaaata ctcatataac catattcttt caaaaataca tgccattttt
     3901 ctgtttcata ataatcctgg aggctacaag tatggtgata taataaatct tgaaccttgt
     3961 tttactggaa ctcctaaagt caagccatac ataatgccag aaacaggacc tgcgtagata
     4021 acatataaca tgtaccagtc atgattaccc aacgtgccgt tacaaatgat atatagacct
     4081 acattttagt tagtttcgtc gtaagttatg gtaataaagg aaaagtcctc taatgaatct
     4141 ataatatagg ttcacacaaa taagagtgat aattaaaagc gtattcgaca ctgaaagatc
     4201 tgctgggaat actatactga tatttccaaa atatccctta taaattgaat ctggaatagc
     4261 acttcttttt ttaaaaaacc ttgaattgtt ggtagcattt ctatcctccc actatgagtt
     4321 taattaacag atccatcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     4381 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     4441 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca
     4501 agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg cagtgcttcg
     4561 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     4621 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     4681 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     4741 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     4801 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     4861 agtagggcgc gccacttcta aataagcgaa tttcttatga tttatgattt ttattattaa
     4921 ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg
     4981 aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagta
     5041 tgaggtcgct cttattgacc acacctctac cggcagatcc gctagggata acagggtaat
     5101 atagatccgt cgacctgcag cgtacgaagc ttcagctggc ggccgcgt
//
