LOCUS       40924_19376        4329 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid for swapping in the GAL1 promoter using the HIS3MX6 
            selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 4329)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4329)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(87..528)
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     gene            662..1862
                     /label=HIS3MX6
                     /note="yeast selectable marker encoding the S. pombe his5
                     gene, which corresponds to S. cerevisiae HIS3"
     promoter        complement(1967..1985)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2243..2831)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3005..3862)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3863..3967)
                     /label=AmpR promoter
     promoter        4313..4329
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 aagacatttt gagatccggg ttttttctcc ttgacgttaa agtatagagg tatattaaca
      121 attttttgtt gatactttta ttacatttga ataagaagta atacaaaccg aaaatgttga
      181 aagtattagt taaagtggtt atgcagtttt tgcatttata tatctgttaa tagatcaaaa
      241 atcatcgctt cgctgattaa ttaccccaga aataaggcta aaaaactaat cgcattatca
      301 tcctatggtt gttaatttga ttcgttcatt tgaaggtttg tggggccagg ttactgccaa
      361 tttttcctct tcataaccat aaaagctagt attgtagaat ctttattgtt cggagcagtg
      421 cggcgcgagg cacatctgcg tttcaggaac gcgaccggtg aagacgagga cgcacggagg
      481 agagtcttcc ttcggagggc tgtcacccgc tcggcggctt ctaatccgta cttcaatata
      541 gcaatgagca gttaagcgta ttactgaaag ttccaaagag aaggtttttt taggctaaga
      601 taatggggct ctttacagat ctgtttagct tgcctcgtcc ccgccgggtc acccggccag
      661 cgacatggag gcccagaata ccctccttga cagtcttgac gtgcgcagct caggggcatg
      721 atgtgactgt cgcccgtaca tttagcccat acatccccat gtataatcat ttgcatccat
      781 acattttgat ggccgcacgg cgcgaagcaa aaattacggc tcctcgctgc agacctgcga
      841 gcagggaaac gctcccctca cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag
      901 aaatataaaa ggttaggatt tgccactgag gttcttcttt catatacttc cttttaaaat
      961 cttgctagga tacagttctc acatcacatc cgaacataaa caaccatggg taggagggct
     1021 tttgtagaaa gaaatacgaa cgaaacgaaa atcagcgttg ccatcgcttt ggacaaagct
     1081 cccttacctg aagagtcgaa ttttattgat gaacttataa cttccaagca tgcaaaccaa
     1141 aagggagaac aagtaatcca agtagacacg ggaattggat tcttggatca catgtatcat
     1201 gcactggcta aacatgcagg ctggagctta cgactttact caagaggtga tttaatcatc
     1261 gatgatcatc acactgcaga agatactgct attgcacttg gtattgcatt caagcaggct
     1321 atgggtaact ttgccggcgt taaaagattt ggacatgctt attgtccact tgacgaagct
     1381 ctttctagaa gcgtagttga cttgtcggga cggccctatg ctgttatcga tttgggatta
     1441 aagcgtgaaa aggttgggga attgtcctgt gaaatgatcc ctcacttact atattccttt
     1501 tcggtagcag ctggaattac tttgcatgtt acctgcttat atggtagtaa tgaccatcat
     1561 cgtgctgaaa gcgcttttaa atctctggct gttgccatgc gcgcggctac tagtcttact
     1621 ggaagttctg aagtcccaag cacgaaggga gtgttgtaaa ggatactgac aataaaaaga
     1681 ttcttgtttt caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt
     1741 aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga
     1801 agttaagtgc gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac
     1861 tgctgtcgat tcgatactaa cgccgccatc cagtttaaac gagctcgaat tcatcgatga
     1921 tatcagatcc actagtggcc tatgcggccg cggatctgcc ggtctcccta tagtgagtcg
     1981 tattaatttc gataagccag gttaacctgc attaatgaat cggccaacgc gcggggagag
     2041 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2101 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2161 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2221 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2281 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2341 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2401 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
     2461 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2521 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2581 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2641 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     2701 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2761 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2821 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2881 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     2941 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3001 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3061 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3121 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3181 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3241 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3301 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3361 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3421 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3481 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     3541 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     3601 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     3661 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     3721 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     3781 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3841 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     3901 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     3961 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     4021 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     4081 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     4141 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     4201 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg gacatattgt
     4261 cgttagaacg cggctacaat taatacataa ccttatgtat catacacata cgatttaggt
     4321 gacactata
//
