LOCUS       pFA6a-His3MX6-PG        5209 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid with a HIS3MX6 marker for swapping in the GAL1 promoter and 
            adding a GST tag.
ACCESSION   .
VERSION     .
KEYWORDS    pFA6a-His3MX6-PGAL1-GST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 5209)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5209)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(50..237)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(279..932)
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             complement(945..947)
                     /codon_start=1
                     /label=start codon
                     /note="start codon"
                     /translation="M"
     promoter        complement(967..1408)
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     gene            1542..2742
                     /label=HIS3MX6
                     /note="yeast selectable marker encoding the S. pombe his5
                     gene, which corresponds to S. cerevisiae HIS3"
     promoter        complement(2847..2865)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3123..3711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3885..4742)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4743..4847)
                     /label=AmpR promoter
     promoter        5193..5209
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatctgc cggtagaggt
       61 gtggtcaata agagcgacct catgctatac ctgagaaagc aacctgacct acaggaaaga
      121 gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa atttgtatac
      181 acttattttt tttataactt atttaataat aaaaatcata aatcataaga aattcgctta
      241 tttagaagtg gcgcgcctca acgcggaacc agatccgatt ttggaggatg gtcgccacca
      301 ccaaacgtgg cttgccagcc ctgcaaaggc catgctatat acttgctgga tttcaagtac
      361 ttatcaattt gtgggatagc ttcaatacgt tttttaaaac aaactaattt tgggaacgca
      421 tccaggcaca ttgggtccat gtataaaaca acatcaagag cgtcatacaa catgaagtca
      481 ggatgggtta catgatcacc atttaaatat gttttatgac ataaacgatc ttcgaacatt
      541 ttcagcattt caggtagctt gctaagaaaa tcaactttga gagtttcaaa gtctttacta
      601 tatgcaattc tcgaaacacc gtatctaata tccaaaaccg ctccttcaag cattgaaatc
      661 tctgcacgct cttttggaca accacccaac atgttgtgct tgtcagctat ataacgtatg
      721 atggccatag actgtgttaa tttaacatca ccatcaatat aataaggaag attgggaaac
      781 tccaaaccca attcaaactt tttgtttcgc catttatcac cttcatcgcg ctcatacaaa
      841 tgctcttcat atttttcttc aagatattcc aaaagaagtc gagtgggttg cacaaggccc
      901 ttaattttcc aataacctag tataggggac atattaatta aagacatttt gagatccggg
      961 ttttttctcc ttgacgttaa agtatagagg tatattaaca attttttgtt gatactttta
     1021 ttacatttga ataagaagta atacaaaccg aaaatgttga aagtattagt taaagtggtt
     1081 atgcagtttt tgcatttata tatctgttaa tagatcaaaa atcatcgctt cgctgattaa
     1141 ttaccccaga aataaggcta aaaaactaat cgcattatca tcctatggtt gttaatttga
     1201 ttcgttcatt tgaaggtttg tggggccagg ttactgccaa tttttcctct tcataaccat
     1261 aaaagctagt attgtagaat ctttattgtt cggagcagtg cggcgcgagg cacatctgcg
     1321 tttcaggaac gcgaccggtg aagacgagga cgcacggagg agagtcttcc ttcggagggc
     1381 tgtcacccgc tcggcggctt ctaatccgta cttcaatata gcaatgagca gttaagcgta
     1441 ttactgaaag ttccaaagag aaggtttttt taggctaaga taatggggct ctttacagat
     1501 ctgtttagct tgcctcgtcc ccgccgggtc acccggccag cgacatggag gcccagaata
     1561 ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtaca
     1621 tttagcccat acatccccat gtataatcat ttgcatccat acattttgat ggccgcacgg
     1681 cgcgaagcaa aaattacggc tcctcgctgc agacctgcga gcagggaaac gctcccctca
     1741 cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt
     1801 tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc
     1861 acatcacatc cgaacataaa caaccatggg taggagggct tttgtagaaa gaaatacgaa
     1921 cgaaacgaaa atcagcgttg ccatcgcttt ggacaaagct cccttacctg aagagtcgaa
     1981 ttttattgat gaacttataa cttccaagca tgcaaaccaa aagggagaac aagtaatcca
     2041 agtagacacg ggaattggat tcttggatca catgtatcat gcactggcta aacatgcagg
     2101 ctggagctta cgactttact caagaggtga tttaatcatc gatgatcatc acactgcaga
     2161 agatactgct attgcacttg gtattgcatt caagcaggct atgggtaact ttgccggcgt
     2221 taaaagattt ggacatgctt attgtccact tgacgaagct ctttctagaa gcgtagttga
     2281 cttgtcggga cggccctatg ctgttatcga tttgggatta aagcgtgaaa aggttgggga
     2341 attgtcctgt gaaatgatcc ctcacttact atattccttt tcggtagcag ctggaattac
     2401 tttgcatgtt acctgcttat atggtagtaa tgaccatcat cgtgctgaaa gcgcttttaa
     2461 atctctggct gttgccatgc gcgcggctac tagtcttact ggaagttctg aagtcccaag
     2521 cacgaaggga gtgttgtaaa ggatactgac aataaaaaga ttcttgtttt caagaacttg
     2581 tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt
     2641 tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta
     2701 atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa
     2761 cgccgccatc cagtttaaac gagctcgaat tcatcgatga tatcagatcc actagtggcc
     2821 tatgcggccg cggatctgcc ggtctcccta tagtgagtcg tattaatttc gataagccag
     2881 gttaacctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     2941 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     3001 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     3061 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3121 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3181 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3241 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3301 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3361 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3421 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3481 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3541 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     3601 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3661 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3721 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3781 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3841 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     3901 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     3961 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     4021 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     4081 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     4141 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     4201 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     4261 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     4321 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     4381 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     4441 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     4501 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     4561 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     4621 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     4681 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     4741 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     4801 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     4861 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     4921 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     4981 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     5041 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     5101 gagcagattg tactgagagt gcaccatatg gacatattgt cgttagaacg cggctacaat
     5161 taatacataa ccttatgtat catacacata cgatttaggt gacactata
//
