LOCUS       pFA6a-His3MX6-PG        5248 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid with a HIS3MX6 marker for swapping in the GAL1 promoter and 
            adding a GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    pFA6a-His3MX6-PGAL1-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 5248)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5248)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(50..237)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(261..971)
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
     CDS             complement(984..986)
                     /codon_start=1
                     /label=start codon
                     /note="start codon"
                     /translation="M"
     promoter        complement(1006..1447)
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     gene            1581..2781
                     /label=HIS3MX6
                     /note="yeast selectable marker encoding the S. pombe his5
                     gene, which corresponds to S. cerevisiae HIS3"
     promoter        complement(2886..2904)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3162..3750)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3924..4781)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4782..4886)
                     /label=AmpR promoter
     promoter        5232..5248
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatctgc cggtagaggt
       61 gtggtcaata agagcgacct catgctatac ctgagaaagc aacctgacct acaggaaaga
      121 gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa atttgtatac
      181 acttattttt tttataactt atttaataat aaaaatcata aatcataaga aattcgctta
      241 tttagaagtg gcgcgcccta tttgtatagt tcatccatgc catgtgtaat cccagcagct
      301 gttacaaact caagaaggac catgtggtct ctcttttcgt tgggatcttt cgaaagggca
      361 gattgtgtgg acaggtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta
      421 ttttgttgat aatggtctgc tagttgaacg cttccatctt caatgttgtg tctaattttg
      481 aagttaactt tgattccatt cttttgtttg tctgccatga tgtatacatt gtgtgagtta
      541 tagttgtatt ccaatttgtg tccaagaatg tttccatctt ctttaaaatc aatacctttt
      601 aactcgattc tattaacaag ggtatcacct tcaaacttga cttcagcacg tgtcttgtag
      661 ttcccgtcat ctttgaaaaa tatagttctt tcctgtacat aaccttcggg catggcactc
      721 ttgaaaaagt catgccgttt catatgatct gggtatcttg aaaagcattg aacaccatac
      781 gtaaaagtag tgacaagtgt tggccatgga acaggtagtt ttccagtagt gcaaataaat
      841 ttaagggtaa gttttccgta tgttgcatca ccttcaccct ctccactgac agaaaatttg
      901 tgcccattaa catcaccatc taattcaaca agaattggga caactccagt gaaaagttct
      961 tctcctttac tgttaattaa agacattttg agatccgggt tttttctcct tgacgttaaa
     1021 gtatagaggt atattaacaa ttttttgttg atacttttat tacatttgaa taagaagtaa
     1081 tacaaaccga aaatgttgaa agtattagtt aaagtggtta tgcagttttt gcatttatat
     1141 atctgttaat agatcaaaaa tcatcgcttc gctgattaat taccccagaa ataaggctaa
     1201 aaaactaatc gcattatcat cctatggttg ttaatttgat tcgttcattt gaaggtttgt
     1261 ggggccaggt tactgccaat ttttcctctt cataaccata aaagctagta ttgtagaatc
     1321 tttattgttc ggagcagtgc ggcgcgaggc acatctgcgt ttcaggaacg cgaccggtga
     1381 agacgaggac gcacggagga gagtcttcct tcggagggct gtcacccgct cggcggcttc
     1441 taatccgtac ttcaatatag caatgagcag ttaagcgtat tactgaaagt tccaaagaga
     1501 aggttttttt aggctaagat aatggggctc tttacagatc tgtttagctt gcctcgtccc
     1561 cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac agtcttgacg
     1621 tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata catccccatg
     1681 tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa aattacggct
     1741 cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg aattgtcccc
     1801 acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg ttcttctttc
     1861 atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc gaacataaac
     1921 aaccatgggt aggagggctt ttgtagaaag aaatacgaac gaaacgaaaa tcagcgttgc
     1981 catcgctttg gacaaagctc ccttacctga agagtcgaat tttattgatg aacttataac
     2041 ttccaagcat gcaaaccaaa agggagaaca agtaatccaa gtagacacgg gaattggatt
     2101 cttggatcac atgtatcatg cactggctaa acatgcaggc tggagcttac gactttactc
     2161 aagaggtgat ttaatcatcg atgatcatca cactgcagaa gatactgcta ttgcacttgg
     2221 tattgcattc aagcaggcta tgggtaactt tgccggcgtt aaaagatttg gacatgctta
     2281 ttgtccactt gacgaagctc tttctagaag cgtagttgac ttgtcgggac ggccctatgc
     2341 tgttatcgat ttgggattaa agcgtgaaaa ggttggggaa ttgtcctgtg aaatgatccc
     2401 tcacttacta tattcctttt cggtagcagc tggaattact ttgcatgtta cctgcttata
     2461 tggtagtaat gaccatcatc gtgctgaaag cgcttttaaa tctctggctg ttgccatgcg
     2521 cgcggctact agtcttactg gaagttctga agtcccaagc acgaagggag tgttgtaaag
     2581 gatactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat
     2641 attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac
     2701 atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg
     2761 tgaatgctgg tcgctatact gctgtcgatt cgatactaac gccgccatcc agtttaaacg
     2821 agctcgaatt catcgatgat atcagatcca ctagtggcct atgcggccgc ggatctgccg
     2881 gtctccctat agtgagtcgt attaatttcg ataagccagg ttaacctgca ttaatgaatc
     2941 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     3001 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3061 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     3121 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3181 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3241 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3301 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     3361 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3421 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3481 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3541 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3601 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3661 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3721 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3781 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3841 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3901 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3961 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4021 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4081 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4141 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4201 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     4261 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4321 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4381 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4441 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4501 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4561 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     4621 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4681 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4741 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4801 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4861 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     4921 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     4981 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     5041 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     5101 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     5161 caccatatgg acatattgtc gttagaacgc ggctacaatt aatacataac cttatgtatc
     5221 atacacatac gatttaggtg acactata
//
