LOCUS       pFA6a-His3MX6-PG        4642 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid with a HIS3MX6 marker for swapping in the GAL1 promoter and 
            adding a triple-HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    pFA6a-His3MX6-PGAL1-3HA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 4642)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4642)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(50..237)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(270..359)
                     /label=3xHA
                     /note="three tandem HA epitope tags"
     CDS             complement(378..380)
                     /codon_start=1
                     /label=start codon
                     /note="start codon"
                     /translation="M"
     promoter        complement(400..841)
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     gene            975..2175
                     /label=HIS3MX6
                     /note="yeast selectable marker encoding the S. pombe his5
                     gene, which corresponds to S. cerevisiae HIS3"
     promoter        complement(2280..2298)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2556..3144)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3318..4175)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4176..4280)
                     /label=AmpR promoter
     promoter        4626..4642
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatctgc cggtagaggt
       61 gtggtcaata agagcgacct catgctatac ctgagaaagc aacctgacct acaggaaaga
      121 gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa atttgtatac
      181 acttattttt tttataactt atttaataat aaaaatcata aatcataaga aattcgctta
      241 tttagaagtg gcgcgcctca gcactgagca gcgtaatctg gaacgtcata tggataggat
      301 cctgcatagt ccgggacgtc atacggatag cccgcatagt caggaacatc gtatgggtaa
      361 aagatgttaa ttaaagacat tttgagatcc gggttttttc tccttgacgt taaagtatag
      421 aggtatatta acaatttttt gttgatactt ttattacatt tgaataagaa gtaatacaaa
      481 ccgaaaatgt tgaaagtatt agttaaagtg gttatgcagt ttttgcattt atatatctgt
      541 taatagatca aaaatcatcg cttcgctgat taattacccc agaaataagg ctaaaaaact
      601 aatcgcatta tcatcctatg gttgttaatt tgattcgttc atttgaaggt ttgtggggcc
      661 aggttactgc caatttttcc tcttcataac cataaaagct agtattgtag aatctttatt
      721 gttcggagca gtgcggcgcg aggcacatct gcgtttcagg aacgcgaccg gtgaagacga
      781 ggacgcacgg aggagagtct tccttcggag ggctgtcacc cgctcggcgg cttctaatcc
      841 gtacttcaat atagcaatga gcagttaagc gtattactga aagttccaaa gagaaggttt
      901 ttttaggcta agataatggg gctctttaca gatctgttta gcttgcctcg tccccgccgg
      961 gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt gacgtgcgca
     1021 gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc catgtataat
     1081 catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac ggctcctcgc
     1141 tgcagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt ccccacgccg
     1201 cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc tttcatatac
     1261 ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat aaacaaccat
     1321 gggtaggagg gcttttgtag aaagaaatac gaacgaaacg aaaatcagcg ttgccatcgc
     1381 tttggacaaa gctcccttac ctgaagagtc gaattttatt gatgaactta taacttccaa
     1441 gcatgcaaac caaaagggag aacaagtaat ccaagtagac acgggaattg gattcttgga
     1501 tcacatgtat catgcactgg ctaaacatgc aggctggagc ttacgacttt actcaagagg
     1561 tgatttaatc atcgatgatc atcacactgc agaagatact gctattgcac ttggtattgc
     1621 attcaagcag gctatgggta actttgccgg cgttaaaaga tttggacatg cttattgtcc
     1681 acttgacgaa gctctttcta gaagcgtagt tgacttgtcg ggacggccct atgctgttat
     1741 cgatttggga ttaaagcgtg aaaaggttgg ggaattgtcc tgtgaaatga tccctcactt
     1801 actatattcc ttttcggtag cagctggaat tactttgcat gttacctgct tatatggtag
     1861 taatgaccat catcgtgctg aaagcgcttt taaatctctg gctgttgcca tgcgcgcggc
     1921 tactagtctt actggaagtt ctgaagtccc aagcacgaag ggagtgttgt aaaggatact
     1981 gacaataaaa agattcttgt tttcaagaac ttgtcatttg tatagttttt ttatattgta
     2041 gttgttctat tttaatcaaa tgttagcgtg atttatattt tttttcgcct cgacatcatc
     2101 tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg
     2161 ctggtcgcta tactgctgtc gattcgatac taacgccgcc atccagttta aacgagctcg
     2221 aattcatcga tgatatcaga tccactagtg gcctatgcgg ccgcggatct gccggtctcc
     2281 ctatagtgag tcgtattaat ttcgataagc caggttaacc tgcattaatg aatcggccaa
     2341 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     2401 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2461 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2521 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2581 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2641 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2701 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc
     2761 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2821 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2881 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2941 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     3001 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3061 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3121 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3181 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3241 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3301 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3361 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3421 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3481 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3541 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3601 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     3661 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3721 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3781 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     3841 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     3901 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     3961 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4021 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4081 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4141 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4201 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4261 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     4321 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     4381 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     4441 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     4501 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     4561 atggacatat tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac
     4621 atacgattta ggtgacacta ta
//
