LOCUS       62056_10695        5162 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        132..475
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      2512..2709
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(2817..2835)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3093..3681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3855..4712)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4713..4817)
                     /label=AmpR promoter
ORIGIN
        1 atttaggtga cactatagaa cgcggccgcc agctgaagct tcgtacgctg caggtcgacg
       61 gatccccggg ttaattaagg cgcgccagat ctgtttagct tgcctcgtcc ccgccgggtc
      121 acccggccag cgacatggag gcccagaata ccctccttga cagtcttgac gtgcgcagct
      181 caggggcatg atgtgactgt cgcccgtaca tttagcccat acatccccat gtataatcat
      241 ttgcatccat acattttgat ggccgcacgg cgcgaagcaa aaattacggc tcctcgctgc
      301 agacctgcga gcagggaaac gctcccctca cagacgcgtt gaattgtccc cacgccgcgc
      361 ccctgtagag aaatataaaa ggttaggatt tgccactgag gttcttcttt catatacttc
      421 cttttaaaat cttgctagga tacagttctc acatcacatc cgaacataaa caaccatggc
      481 tagctttcaa cgttttcttt actattgcac ttataattgt atttggaaat gaaagacata
      541 tggagcttta tttctctttc ctcttcaggt ttctgaattt attggatata atgacacggt
      601 gatacacgtt gtaatgaatt aaagactcac attgctacac actatatatt tatagatatc
      661 acctgcatct taggttaatt attaattaat ctcatcgatc gctttcttct tactttaaca
      721 aaaacacatc gaaagagata cgatgtttga tttgaatact tgtcttcgta aaaatatctt
      781 agaacttcag ccttatcgct gtgcgagaga gtatgtaata acttgataaa ctagaaagcg
      841 tgatatatat taacatagaa tatagtgact tctctgagta tgttttatct ttatatttgg
      901 gtaataattg atatgagggc tacttctaga aagatatttt attagctgct tggaaagcaa
      961 tgaagtactc cctgtttact gttgttgttg gagctggcga tttcaggcct ttgttgtttt
     1021 ttaccctttt ctcgcctata tattttttgc taatcttaat agaggtgtcc ttcttgatgc
     1081 caatgaatgt gcttatggtt cggttatttc cgtggacggt gtggaattca atcgttatcc
     1141 ggatcctaga cagatcgaag taaagcaaag attatgtgat ttgagaaaca aagagctttc
     1201 cattaccaag ccactaacac cagataacat ttgcatgggt gttggcagcg atgaaattat
     1261 tgactcctta attcgcattt cttgtattcc tggaaaagat aagattttga tgtgtccacc
     1321 ttcgtacgga atgtatacgg tctctgcaaa aatcaacgat gttgaggttg ttaaggttct
     1381 ccttgaacca gatttcaatt tgaacgtcga tgctatttgc gaaacacttt ctaaagatag
     1441 tgccatcaaa gtcttttttg cttgttcccc tggtaatcca acagctaagg ctcttaaact
     1501 cgaggatatt aagaagattt tggagcaccc tacatggaat ggaatcgttg tcgtcgatga
     1561 ggcctatatt gatttctctg ctcccgatat gtctgcctta actcttgtca atgaatatcc
     1621 aaaccttgct gtttgccaaa ccctttcgaa atctttcggt ctagcaggaa ttcggtaagt
     1681 taatctaagc ttttaagcat taatattatt tctttactaa ctatgatgtt tagtatcgga
     1741 ttttgtttga caagtaaacc tattgctacc attatgaact cattaaaggc tccttataac
     1801 attagcgaac ccacctctcg tttagctttg gatgctttat cccctcagtc gattgataaa
     1861 atgcatacat atagagatgc tattatacag caaagagttc gtctttgcaa agagcttact
     1921 accattaaag gaatgggcaa gattataggt ggttatgatg caaattttat cctcattcag
     1981 gttttagata gaccagaagg aggtaagcct agtaacgatg ccgcaaaata tctttattta
     2041 caaatggcca cgatgcacaa agtcgttgtt agatttagag gtacagagcc tctttgcgaa
     2101 ggagcattgc gtatcactgt tggcacagaa gaagagaata cgatattact aaaaactatt
     2161 aaacttgtat tgcaggaata ctatacaaaa aaatgaggtt tccttttctg gcctattaga
     2221 acgtttcaat tagacaaatt ataactcttt cctttcccct tccggactcg tcacatttct
     2281 tttctgatgt acccaatata ttttgtgttc gctaattgcg cttgcattcc ttctttcttt
     2341 attaaaatac gttttcttcc tttttggtgt gtttccgtat agttcgataa gcaatgaaaa
     2401 gatgttattt ttaattttgt aaaggagtta cgctaattta aaggattata acccgtgtaa
     2461 taaaagaatt aaagattcgt tagctttgca tagagctagc ccctaatcag tactgacaat
     2521 aaaaagattc ttgttttcaa gaacttgtca tttgtatagt ttttttatat tgtagttgtt
     2581 ctattttaat caaatgttag cgtgatttat attttttttc gcctcgacat catctgccca
     2641 gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc
     2701 gctatactgc tgtcgattcg atactaacgc cgccatccag tgtcgaaaac gagctcgaat
     2761 tcatcgatga tatcagatcc actagtggcc tatgcggccg cggatctgcc ggtctcccta
     2821 tagtgagtcg tattaatttc gataagccag gttaacctgc attaatgaat cggccaacgc
     2881 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     2941 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     3001 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     3061 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     3121 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     3181 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     3241 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     3301 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     3361 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     3421 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     3481 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     3541 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     3601 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3661 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3721 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3781 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3841 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3901 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3961 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     4021 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     4081 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     4141 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     4201 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     4261 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     4321 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     4381 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     4441 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     4501 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     4561 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     4621 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     4681 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4741 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4801 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     4861 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     4921 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     4981 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     5041 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     5101 gacatattgt cgttagaacg cggctacaat taatacataa ccttatgtat catacacata
     5161 cg
//
