LOCUS       40924_19431        3993 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFA6a-HTB-TRP1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, 
            Auer B, Huang L, Kaiser P.
  TITLE     HB tag modules for PCR-based gene tagging and tandem affinity 
            purification in Saccharomyces cerevisiae
  JOURNAL   Yeast 23 (8), 623-632 (2006)
  PUBMED    16823883
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Kaiser P, Tagwerker C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2006) Biological Chemistry, UC Irvine, 19182 
            Jamboree Blvd RM H136 Plumwood Bldg, Irvine, CA 92697, USA
REFERENCE   3  (bases 1 to 3993)
  AUTHORS   Kaiser P, Tagwerker C.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2006) Biological Chemistry, UC Irvine, 19182 
            Jamboree Blvd RM H136 Plumwood Bldg, Irvine, CA 92697, USA
REFERENCE   4  (bases 1 to 3993)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3993)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2006"; volume: "23"; issue: "8"; pages: "623-632"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-FEB-2006) Biological Chemistry, UC Irvine, 19182 Jamboree Blvd 
            RM H136 Plumwood Bldg, Irvine, CA 92697, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (18-JUL-2006) Biological Chemistry, UC Irvine, 19182 Jamboree Blvd 
            RM H136 Plumwood Bldg, Irvine, CA 92697, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     On Jul 18, 2006 this sequence version replaced DQ407923.1.
FEATURES             Location/Qualifiers
     source          1..3993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             72..89
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             117..137
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQS"
     terminator      431..618
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        654..801
                     /label=TRP1 promoter
     CDS             802..1473
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     RBS             1550..1558
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(1631..1649)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1907..2495)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2669..3526)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3527..3631)
                     /label=AmpR promoter
     promoter        3977..3993
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 acaggggttc acatcatcac caccatcatg actacgatat acccacaacc gccagcgaga
      121 atctgtactt tcaatcagag ttaaaaaccg ccgcactagc tcaacatgac gaggctgctg
      181 gaaaggccgg tgaaggtgaa atccctgccc ctcttgctgg taccgtttct aagatactgg
      241 taaaagaagg tgacactgtt aaagctggtc aaacagttct ggtgctggag gctatgaaaa
      301 tggagacaga aattaacgct cctactgacg gaaaagttga aaaggtgtta gttaaggaaa
      361 gagatgctgt tcaaggtggt caaggtctaa tcaagatcgg cgtttaatga ggcgcgccac
      421 ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa
      481 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt
      541 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg tcgctcttat
      601 tgaccacacc tctaccggca gatccgctag ggataacagg gtaatataga tctgtacaat
      661 cttgatccgg agcttttctt tttttgccga ttaagaatta attcggtcga aaaaagaaaa
      721 ggagagggcc aagagggagg gcattggtga ctattgagca cgtgagtata cgtgattaag
      781 cacacaaagg cagcttggag tatgtctgtt attaatttca caggtagttc tggtccattg
      841 gtgaaagttt gcggcttgca gagcacagag gccgcagaat gtgctctaga ttccgatgct
      901 gacttgctgg gtattatatg tgtgcccaat agaaagagaa caattgaccc ggttattgca
      961 aggaaaattt caagtcttgt aaaagcatat aaaaatagtt caggcactcc gaaatacttg
     1021 gttggcgtgt ttcgtaatca acctaaggag gatgttttgg ctctggtcaa tgattacggc
     1081 attgatatcg tccaactgca tggagatgag tcgtggcaag aataccaaga gttcctcggt
     1141 ttgccagtta ttaaaagact cgtatttcca aaagactgca acatactact cagtgcagct
     1201 tcacagaaac ctcattcgtt tattcccttg tttgattcag aagcaggtgg gacaggtgaa
     1261 cttttggatt ggaactcgat ttctgactgg gttggaaggc aagagagccc cgaaagctta
     1321 cattttatgt tagctggtgg actgacgcca gaaaatgttg gtgatgcgct tagattaaat
     1381 ggcgttattg gtgttgatgt aagcggaggt gtggagacaa atggtgtaaa agactctaac
     1441 aaaatagcaa atttcgtcaa aaatgctaag aaataggtta ttactgagta gtatttattt
     1501 aagtattgtt tgtgcacttg cctgcggtgt gaaataccgc acagatgcgt aaggaggttt
     1561 aaacgagctc gaattcatcg atgatatcag atccactagt ggcctatgcg gccgcggatc
     1621 tgccggtctc cctatagtga gtcgtattaa tttcgataag ccaggttaac ctgcattaat
     1681 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     1741 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1801 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1861 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1921 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1981 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2041 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2101 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2161 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2221 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2281 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2341 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2401 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2461 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2521 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2581 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     2641 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     2701 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     2761 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     2821 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     2881 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     2941 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3001 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3061 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3121 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3181 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     3241 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     3301 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3361 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3421 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     3481 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     3541 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     3601 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     3661 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     3721 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     3781 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     3841 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     3901 gagtgcacca tatggacata ttgtcgttag aacgcggcta caattaatac ataaccttat
     3961 gtatcataca catacgattt aggtgacact ata
//
