LOCUS       40924_19306        4570 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast tagging vector pFA6a-HA-KlURA3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Sung M-K., Huh W-K.
  TITLE     Yeast tagging vector pFA6a-HA-KlURA3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Sung M-K., Huh W-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2007) School of Biological Sciences, Seoul 
            National University, Seoul 151-747, Republic of Korea
REFERENCE   3  (bases 1 to 4570)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4570)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-OCT-2007) School of Biological Sciences, Seoul National 
            University, Seoul 151-747, Republic of Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             100..126
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             133..159
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             807..1610
                     /codon_start=1
                     /gene="URA3"
                     /product="orotidine 5'-phosphate decarboxylase"
                     /label=URA3
                     /protein_id="ABX47177.1"
                     /translation="MSTKSYTSRAETHASPVASKLLRLMDEKKTNLCASLDVRSTDELL
                     KLVETLGPYICLLKTHVDILDDFSYEGTVVPLKALAEKYKFLIFEDRKFADIGNTVKLQ
                     YTSGVYRIAEWSDITNAHGVTGAGIVAGLKQGAQEVTKEPRGLLMLAELSSKGSLAHGE
                     YTKGTVDIAKSDKDFVIGFIAQNDMGGREEGFDWLIMTPGVGLDDKGDALGQQYRTVDE
                     VVSGGSDIIIVGRGLFAKGRDPKVEGERYRNAGWEAYQKRISAPH"
     gene            807..1610
                     /gene="URA3"
                     /label=URA3
     terminator      1906..2103
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(2208..2226)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2484..3072)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3246..4103)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4104..4208)
                     /label=AmpR promoter
     promoter        4554..4570
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 acatctttta cccatacgat gtctcctgac tatgcgggct atccctatga cgtcccggac
      121 tatgcaggat cctatccata tgacgttcca gattacgctg ctcagtgctg aggcgcgcct
      181 ctctgctttt gtgcgcgtat gtttatgtat gtacctctct ctctatttct atttttaaac
      241 caccctctca ataaaataaa aataataaag tatttttaag gaaaagacgt gtttaagcac
      301 tgactttatc tactttttgt acgttttcat tgatataatg tgttttgtct ctcccttttc
      361 tacgaaaatt tcaaaaattg accaaaaaaa ggaatatata tacgaaaaac tattatattt
      421 atatatcata gtgttgataa aaaatgttta tcagatctcg gagacaatca tatgggagaa
      481 gcaattggaa gatagaaaaa aggtactcgg tacataaata tatgtgattc tgggtagaag
      541 atcggtctgc attggatggt ggtaacgcat ttttttacac acattacttg cctcgagcat
      601 caaatggtgg ttattcgtgg atctatatca cgtgatttgc ttaagaattg tcgttcatgg
      661 tgacactttt agctttgaca tgattaagct catctcaatt gatgttatct aaagtcattt
      721 caactatcta agatgtggtt gtgattgggc cattttgtga aagccagtac gccagcgtca
      781 atacactccc gtcaattagt tgcaccatgt ccacaaaatc atataccagt agagctgaga
      841 ctcatgcaag tccggttgca tcgaaacttt tacgtttaat ggatgaaaag aagaccaatt
      901 tgtgtgcttc tcttgacgtt cgttcgactg atgagctatt gaaacttgtt gaaacgttgg
      961 gtccatacat ttgccttttg aaaacacacg ttgatatctt ggatgatttc agttatgagg
     1021 gtactgtcgt tccattgaaa gcattggcag agaaatacaa gttcttgata tttgaggaca
     1081 gaaaattcgc cgatatcggt aacacagtca aattacaata tacatcgggc gtttaccgta
     1141 tcgcagaatg gtctgatatc accaacgccc acggggttac tggtgctggt attgttgctg
     1201 gcttgaaaca aggtgcgcaa gaggtcacca aagaaccaag gggattattg atgcttgctg
     1261 aattgtcttc caagggttct ctagcacacg gtgaatatac taagggtacc gttgatattg
     1321 caaagagtga taaagatttc gttattgggt tcattgctca gaacgatatg ggaggaagag
     1381 aagaagggtt tgattggcta atcatgaccc caggtgtagg tttagacgac aaaggcgatg
     1441 cattgggtca gcagtacaga accgtcgacg aagttgtaag tggtggatca gatatcatca
     1501 ttgttggcag aggacttttc gccaagggta gagatcctaa ggttgaaggt gaaagataca
     1561 gaaatgctgg atgggaagcg taccaaaaga gaatcagcgc tccccattaa ttatacagga
     1621 aacttaatag aacaaatcac atatttaatc taatagccac ctgcattggc acggtgcaac
     1681 actcacttca acttcatctt acaaaagatc acgtgatctg ttgtattgaa ctgaaaattt
     1741 tttgtttgct tctctctctc tctttcatta tgtgagagtt taaaaaccag aaactacatc
     1801 atcgaaaaag agtttaaacc attacaacca ttgcgataag ccctctcaaa cttcctccag
     1861 aaccaatgac gttccctcaa ccaaaggtgt tttgatgtga agagtactga caataaaaag
     1921 attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt tgttctattt
     1981 taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg cccagatgcg
     2041 aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata
     2101 ctgctgtcga ttcgatacta acgccgccat ccagtttaaa cgagctcgaa ttcatcgatg
     2161 atatcagatc cactagtggc ctatgcggcc gcggatctgc cggtctccct atagtgagtc
     2221 gtattaattt cgataagcca ggttaacctg cattaatgaa tcggccaacg cgcggggaga
     2281 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2341 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2401 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2461 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2521 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2581 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2641 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     2701 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2761 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2821 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2881 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     2941 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3001 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3061 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3121 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3181 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3241 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3301 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3361 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3421 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3481 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3541 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3601 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3661 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3721 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3781 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3841 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3901 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3961 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4021 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4081 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4141 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     4201 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     4261 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     4321 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     4381 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     4441 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat ggacatattg
     4501 tcgttagaac gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg
     4561 tgacactata
//
