LOCUS       40924_19291        4815 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid with a kanMX marker for adding a C-terminal GST tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 4815)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4815)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             63..716
                     /codon_start=1
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     terminator      758..945
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     gene            992..2348
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     promoter        complement(2453..2471)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2729..3317)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3491..4348)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4349..4453)
                     /label=AmpR promoter
     promoter        4799..4815
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 atatgtcccc tatactaggt tattggaaaa ttaagggcct tgtgcaaccc actcgacttc
      121 ttttggaata tcttgaagaa aaatatgaag agcatttgta tgagcgcgat gaaggtgata
      181 aatggcgaaa caaaaagttt gaattgggtt tggagtttcc caatcttcct tattatattg
      241 atggtgatgt taaattaaca cagtctatgg ccatcatacg ttatatagct gacaagcaca
      301 acatgttggg tggttgtcca aaagagcgtg cagagatttc aatgcttgaa ggagcggttt
      361 tggatattag atacggtgtt tcgagaattg catatagtaa agactttgaa actctcaaag
      421 ttgattttct tagcaagcta cctgaaatgc tgaaaatgtt cgaagatcgt ttatgtcata
      481 aaacatattt aaatggtgat catgtaaccc atcctgactt catgttgtat gacgctcttg
      541 atgttgtttt atacatggac ccaatgtgcc tggatgcgtt cccaaaatta gtttgtttta
      601 aaaaacgtat tgaagctatc ccacaaattg ataagtactt gaaatccagc aagtatatag
      661 catggccttt gcagggctgg caagccacgt ttggtggtgg cgaccatcct ccaaaatcgg
      721 atctggttcc gcgttgaggc gcgccacttc taaataagcg aatttcttat gatttatgat
      781 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
      841 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
      901 tcaggtatag catgaggtcg ctcttattga ccacacctct accggcagat ctgtttagct
      961 tgcctcgtcc ccgccgggtc acccggccag cgacatggag gcccagaata ccctccttga
     1021 cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtaca tttagcccat
     1081 acatccccat gtataatcat ttgcatccat acattttgat ggccgcacgg cgcgaagcaa
     1141 aaattacggc tcctcgctgc agacctgcga gcagggaaac gctcccctca cagacgcgtt
     1201 gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt tgccactgag
     1261 gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc acatcacatc
     1321 cgaacataaa caaccatggg taaggaaaag actcacgttt cgaggccgcg attaaattcc
     1381 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     1441 gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     1501 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     1561 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     1621 accactgcga tccccggcaa aacagcattc caggtattag aagaatatcc tgattcaggt
     1681 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     1741 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     1801 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     1861 gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt
     1921 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     1981 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     2041 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     2101 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcagt actgacaata
     2161 aaaagattct tgttttcaag aacttgtcat ttgtatagtt tttttatatt gtagttgttc
     2221 tattttaatc aaatgttagc gtgatttata ttttttttcg cctcgacatc atctgcccag
     2281 atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg
     2341 ctatactgct gtcgattcga tactaacgcc gccatccagt ttaaacgagc tcgaattcat
     2401 cgatgatatc agatccacta gtggcctatg cggccgcgga tctgccggtc tccctatagt
     2461 gagtcgtatt aatttcgata agccaggtta acctgcatta atgaatcggc caacgcgcgg
     2521 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2581 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2641 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2701 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2761 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2821 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2881 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
     2941 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3001 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3061 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3121 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     3181 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3241 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3301 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3361 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3421 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3481 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3541 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3601 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3661 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3721 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3781 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3841 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3901 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3961 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4021 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4081 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4141 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4201 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4261 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4321 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4381 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4441 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     4501 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     4561 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     4621 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     4681 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatggaca
     4741 tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat
     4801 ttaggtgaca ctata
//
