LOCUS       40924_19301        4292 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid with a TRP1 marker for adding a C-terminal GST tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 4292)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4292)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             63..716
                     /codon_start=1
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     terminator      758..945
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        953..1100
                     /label=TRP1 promoter
     CDS             1101..1772
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(1930..1948)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2206..2794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2968..3825)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3826..3930)
                     /label=AmpR promoter
     promoter        4276..4292
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 atatgtcccc tatactaggt tattggaaaa ttaagggcct tgtgcaaccc actcgacttc
      121 ttttggaata tcttgaagaa aaatatgaag agcatttgta tgagcgcgat gaaggtgata
      181 aatggcgaaa caaaaagttt gaattgggtt tggagtttcc caatcttcct tattatattg
      241 atggtgatgt taaattaaca cagtctatgg ccatcatacg ttatatagct gacaagcaca
      301 acatgttggg tggttgtcca aaagagcgtg cagagatttc aatgcttgaa ggagcggttt
      361 tggatattag atacggtgtt tcgagaattg catatagtaa agactttgaa actctcaaag
      421 ttgattttct tagcaagcta cctgaaatgc tgaaaatgtt cgaagatcgt ttatgtcata
      481 aaacatattt aaatggtgat catgtaaccc atcctgactt catgttgtat gacgctcttg
      541 atgttgtttt atacatggac ccaatgtgcc tggatgcgtt cccaaaatta gtttgtttta
      601 aaaaacgtat tgaagctatc ccacaaattg ataagtactt gaaatccagc aagtatatag
      661 catggccttt gcagggctgg caagccacgt ttggtggtgg cgaccatcct ccaaaatcgg
      721 atctggttcc gcgttgaggc gcgccacttc taaataagcg aatttcttat gatttatgat
      781 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
      841 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
      901 tcaggtatag catgaggtcg ctcttattga ccacacctct accggcagat ctgtacaatc
      961 ttgatccgga gcttttcttt ttttgccgat taagaattaa ttcggtcgaa aaaagaaaag
     1021 gagagggcca agagggaggg cattggtgac tattgagcac gtgagtatac gtgattaagc
     1081 acacaaaggc agcttggagt atgtctgtta ttaatttcac aggtagttct ggtccattgg
     1141 tgaaagtttg cggcttgcag agcacagagg ccgcagaatg tgctctagat tccgatgctg
     1201 acttgctggg tattatatgt gtgcccaata gaaagagaac aattgacccg gttattgcaa
     1261 ggaaaatttc aagtcttgta aaagcatata aaaatagttc aggcactccg aaatacttgg
     1321 ttggcgtgtt tcgtaatcaa cctaaggagg atgttttggc tctggtcaat gattacggca
     1381 ttgatatcgt ccaactgcat ggagatgagt cgtggcaaga ataccaagag ttcctcggtt
     1441 tgccagttat taaaagactc gtatttccaa aagactgcaa catactactc agtgcagctt
     1501 cacagaaacc tcattcgttt attcccttgt ttgattcaga agcaggtggg acaggtgaac
     1561 ttttggattg gaactcgatt tctgactggg ttggaaggca agagagcccc gaaagcttac
     1621 attttatgtt agctggtgga ctgacgccag aaaatgttgg tgatgcgctt agattaaatg
     1681 gcgttattgg tgttgatgta agcggaggtg tggagacaaa tggtgtaaaa gactctaaca
     1741 aaatagcaaa tttcgtcaaa aatgctaaga aataggttat tactgagtag tatttattta
     1801 agtattgttt gtgcacttgc ctgcggtgtg aaataccgca cagatgcgta aggaggttta
     1861 aacgagctcg aattcatcga tgatatcaga tccactagtg gcctatgcgg ccgcggatct
     1921 gccggtctcc ctatagtgag tcgtattaat ttcgataagc caggttaacc tgcattaatg
     1981 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     2041 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     2101 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     2161 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2221 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2281 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2341 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2401 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2461 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2521 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2581 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2641 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2701 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2761 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2821 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     2881 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     2941 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     3001 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     3061 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     3121 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     3181 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3241 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     3301 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3361 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3421 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     3481 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     3541 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3601 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3661 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3721 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3781 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     3841 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3901 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     3961 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     4021 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     4081 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     4141 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     4201 agtgcaccat atggacatat tgtcgttaga acgcggctac aattaataca taaccttatg
     4261 tatcatacac atacgattta ggtgacacta ta
//
