LOCUS       40924_19296        5137 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast tagging vector pFA6a-GST-KlURA3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Sung M-K., Huh W-K.
  TITLE     Yeast tagging vector pFA6a-GST-KlURA3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   Sung M-K., Huh W-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2007) School of Biological Sciences, Seoul 
            National University, Seoul 151-747, Republic of Korea
REFERENCE   3  (bases 1 to 5137)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5137)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-OCT-2007) School of Biological Sciences, Seoul National 
            University, Seoul 151-747, Republic of Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             88..717
                     /codon_start=1
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
                     /translation="KIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFP
                     NLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYS
                     KDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLD
                     AFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             1374..2177
                     /codon_start=1
                     /gene="URA3"
                     /product="orotidine 5'-phosphate decarboxylase"
                     /label=URA3
                     /protein_id="ABX47173.1"
                     /translation="MSTKSYTSRAETHASPVASKLLRLMDEKKTNLCASLDVRSTDELL
                     KLVETLGPYICLLKTHVDILDDFSYEGTVVPLKALAEKYKFLIFEDRKFADIGNTVKLQ
                     YTSGVYRIAEWSDITNAHGVTGAGIVAGLKQGAQEVTKEPRGLLMLAELSSKGSLAHGE
                     YTKGTVDIAKSDKDFVIGFIAQNDMGGREEGFDWLIMTPGVGLDDKGDALGQQYRTVDE
                     VVSGGSDIIIVGRGLFAKGRDPKVEGERYRNAGWEAYQKRISAPH"
     gene            1374..2177
                     /gene="URA3"
                     /label=URA3
     terminator      2473..2670
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(2775..2793)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3051..3639)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3813..4670)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4671..4775)
                     /label=AmpR promoter
     promoter        5121..5137
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 atatgtcccc tatactaggt tattgtgaaa attaagggcc ttgtgcaacc cactcgactt
      121 cttttggaat atcttgaaga aaaatatgaa gagcatttgt atgagcgcga tgaaggtgat
      181 aaatggcgaa acaaaaagtt tgaattgggt ttggagtttc ccaatcttcc ttattatatt
      241 gatggtgatg ttaaattaac acagtctatg gccatcatac gttatatagc tgacaagcac
      301 aacatgttgg gtggttgtcc aaaagagcgt gcagagattt caatgcttga aggagcggtt
      361 ttggatatta gatacggtgt ttcgagaatt gcatatagta aagactttga aactctcaaa
      421 gttgattttc ttagcaagct acctgaaatg ctgaaaatgt tcgaagatcg tttatgtcat
      481 aaaacatatt taaatggtga tcatgtaacc catcctgact tcatgttgta tgacgctctt
      541 gatgttgttt tatacatgga cccaatgtgc ctggatgcgt tcccaaaatt agtttgtttt
      601 aaaaaacgta ttgaagctat cccacaaatt gataagtact tgaaatccag caagtatata
      661 gcatggcctt tgcagggctg gcaagccacg tttggtggtg gcgaccatcc tccaaaatcg
      721 gatctggttc cgcgttgagg cgcgcctctc tgcttttgtg cgcgtatgtt tatgtatgta
      781 cctctctctc tatttctatt tttaaaccac cctctcaata aaataaaaat aataaagtat
      841 ttttaaggaa aagacgtgtt taagcactga ctttatctac tttttgtacg ttttcattga
      901 tataatgtgt tttgtctctc ccttttctac gaaaatttca aaaattgacc aaaaaaagga
      961 atatatatac gaaaaactat tatatttata tatcatagtg ttgataaaaa atgtttatca
     1021 gatctcggag acaatcatat gggagaagca attggaagat agaaaaaagg tactcggtac
     1081 ataaatatat gtgattctgg gtagaagatc ggtctgcatt ggatggtggt aacgcatttt
     1141 tttacacaca ttacttgcct cgagcatcaa atggtggtta ttcgtggatc tatatcacgt
     1201 gatttgctta agaattgtcg ttcatggtga cacttttagc tttgacatga ttaagctcat
     1261 ctcaattgat gttatctaaa gtcatttcaa ctatctaaga tgtggttgtg attgggccat
     1321 tttgtgaaag ccagtacgcc agcgtcaata cactcccgtc aattagttgc accatgtcca
     1381 caaaatcata taccagtaga gctgagactc atgcaagtcc ggttgcatcg aaacttttac
     1441 gtttaatgga tgaaaagaag accaatttgt gtgcttctct tgacgttcgt tcgactgatg
     1501 agctattgaa acttgttgaa acgttgggtc catacatttg ccttttgaaa acacacgttg
     1561 atatcttgga tgatttcagt tatgagggta ctgtcgttcc attgaaagca ttggcagaga
     1621 aatacaagtt cttgatattt gaggacagaa aattcgccga tatcggtaac acagtcaaat
     1681 tacaatatac atcgggcgtt taccgtatcg cagaatggtc tgatatcacc aacgcccacg
     1741 gggttactgg tgctggtatt gttgctggct tgaaacaagg tgcgcaagag gtcaccaaag
     1801 aaccaagggg attattgatg cttgctgaat tgtcttccaa gggttctcta gcacacggtg
     1861 aatatactaa gggtaccgtt gatattgcaa agagtgataa agatttcgtt attgggttca
     1921 ttgctcagaa cgatatggga ggaagagaag aagggtttga ttggctaatc atgaccccag
     1981 gtgtaggttt agacgacaaa ggcgatgcat tgggtcagca gtacagaacc gtcgacgaag
     2041 ttgtaagtgg tggatcagat atcatcattg ttggcagagg acttttcgcc aagggtagag
     2101 atcctaaggt tgaaggtgaa agatacagaa atgctggatg ggaagcgtac caaaagagaa
     2161 tcagcgctcc ccattaatta tacaggaaac ttaatagaac aaatcacata tttaatctaa
     2221 tagccacctg cattggcacg gtgcaacact cacttcaact tcatcttaca aaagatcacg
     2281 tgatctgttg tattgaactg aaaatttttt gtttgcttct ctctctctct ttcattatgt
     2341 gagagtttaa aaaccagaaa ctacatcatc gaaaaagagt ttaaaccatt acaaccattg
     2401 cgataagccc tctcaaactt cctccagaac caatgacgtt ccctcaacca aaggtgtttt
     2461 gatgtgaaga gtactgacaa taaaaagatt cttgttttca agaacttgtc atttgtatag
     2521 tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt
     2581 cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc
     2641 aatcgtatgt gaatgctggt cgctatactg ctgtcgattc gatactaacg ccgccatcca
     2701 gtttaaacga gctcgaattc atcgatgata tcagatccac tagtggccta tgcggccgcg
     2761 gatctgccgg tctccctata gtgagtcgta ttaatttcga taagccaggt taacctgcat
     2821 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     2881 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2941 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     3001 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3061 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3121 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3181 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3241 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3301 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3361 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3421 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3481 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3541 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3601 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3661 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3721 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3781 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3841 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3901 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3961 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4021 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4081 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4141 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4201 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4261 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4321 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4381 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4441 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4501 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4561 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4621 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4681 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4741 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4801 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     4861 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     4921 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     4981 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     5041 ctgagagtgc accatatgga catattgtcg ttagaacgcg gctacaatta atacataacc
     5101 ttatgtatca tacacatacg atttaggtga cactata
//
