LOCUS       40924_19286        4659 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid with a HIS3MX6 marker for adding a C-terminal GST tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 4659)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4659)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             63..716
                     /codon_start=1
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     terminator      758..945
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     gene            992..2192
                     /label=HIS3MX6
                     /note="yeast selectable marker encoding the S. pombe his5
                     gene, which corresponds to S. cerevisiae HIS3"
     promoter        complement(2297..2315)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2573..3161)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3335..4192)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4193..4297)
                     /label=AmpR promoter
     promoter        4643..4659
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 atatgtcccc tatactaggt tattggaaaa ttaagggcct tgtgcaaccc actcgacttc
      121 ttttggaata tcttgaagaa aaatatgaag agcatttgta tgagcgcgat gaaggtgata
      181 aatggcgaaa caaaaagttt gaattgggtt tggagtttcc caatcttcct tattatattg
      241 atggtgatgt taaattaaca cagtctatgg ccatcatacg ttatatagct gacaagcaca
      301 acatgttggg tggttgtcca aaagagcgtg cagagatttc aatgcttgaa ggagcggttt
      361 tggatattag atacggtgtt tcgagaattg catatagtaa agactttgaa actctcaaag
      421 ttgattttct tagcaagcta cctgaaatgc tgaaaatgtt cgaagatcgt ttatgtcata
      481 aaacatattt aaatggtgat catgtaaccc atcctgactt catgttgtat gacgctcttg
      541 atgttgtttt atacatggac ccaatgtgcc tggatgcgtt cccaaaatta gtttgtttta
      601 aaaaacgtat tgaagctatc ccacaaattg ataagtactt gaaatccagc aagtatatag
      661 catggccttt gcagggctgg caagccacgt ttggtggtgg cgaccatcct ccaaaatcgg
      721 atctggttcc gcgttgaggc gcgccacttc taaataagcg aatttcttat gatttatgat
      781 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
      841 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
      901 tcaggtatag catgaggtcg ctcttattga ccacacctct accggcagat ctgtttagct
      961 tgcctcgtcc ccgccgggtc acccggccag cgacatggag gcccagaata ccctccttga
     1021 cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtaca tttagcccat
     1081 acatccccat gtataatcat ttgcatccat acattttgat ggccgcacgg cgcgaagcaa
     1141 aaattacggc tcctcgctgc agacctgcga gcagggaaac gctcccctca cagacgcgtt
     1201 gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt tgccactgag
     1261 gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc acatcacatc
     1321 cgaacataaa caaccatggg taggagggct tttgtagaaa gaaatacgaa cgaaacgaaa
     1381 atcagcgttg ccatcgcttt ggacaaagct cccttacctg aagagtcgaa ttttattgat
     1441 gaacttataa cttccaagca tgcaaaccaa aagggagaac aagtaatcca agtagacacg
     1501 ggaattggat tcttggatca catgtatcat gcactggcta aacatgcagg ctggagctta
     1561 cgactttact caagaggtga tttaatcatc gatgatcatc acactgcaga agatactgct
     1621 attgcacttg gtattgcatt caagcaggct atgggtaact ttgccggcgt taaaagattt
     1681 ggacatgctt attgtccact tgacgaagct ctttctagaa gcgtagttga cttgtcggga
     1741 cggccctatg ctgttatcga tttgggatta aagcgtgaaa aggttgggga attgtcctgt
     1801 gaaatgatcc ctcacttact atattccttt tcggtagcag ctggaattac tttgcatgtt
     1861 acctgcttat atggtagtaa tgaccatcat cgtgctgaaa gcgcttttaa atctctggct
     1921 gttgccatgc gcgcggctac tagtcttact ggaagttctg aagtcccaag cacgaaggga
     1981 gtgttgtaaa ggatactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat
     2041 agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt
     2101 ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta atatcatgcg
     2161 tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa cgccgccatc
     2221 cagtttaaac gagctcgaat tcatcgatga tatcagatcc actagtggcc tatgcggccg
     2281 cggatctgcc ggtctcccta tagtgagtcg tattaatttc gataagccag gttaacctgc
     2341 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     2401 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2461 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2521 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2581 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2641 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2701 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2761 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2821 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2881 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2941 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3001 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3061 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3121 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3181 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3241 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3301 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3361 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3421 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3481 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3541 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3601 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3661 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3721 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3781 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3841 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3901 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3961 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     4021 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4081 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4141 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4201 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4261 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4321 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     4381 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     4441 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     4501 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     4561 tactgagagt gcaccatatg gacatattgt cgttagaacg cggctacaat taatacataa
     4621 ccttatgtat catacacata cgatttaggt gacactata
//
