LOCUS       40924_19281        5175 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast tagging vector pFA6a-GFP-KlURA3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Sung M-K., Huh W-K.
  TITLE     Yeast tagging vector pFA6a-GFP-KlURA3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Sung M-K., Huh W-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2007) School of Biological Sciences, Seoul 
            National University, Seoul 151-747, Republic of Korea
REFERENCE   3  (bases 1 to 5175)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5175)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-OCT-2007) School of Biological Sciences, Seoul National 
            University, Seoul 151-747, Republic of Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             63..773
                     /codon_start=1
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             1412..2215
                     /codon_start=1
                     /gene="URA3"
                     /product="orotidine 5'-phosphate decarboxylase"
                     /label=URA3
                     /protein_id="ABX47169.1"
                     /translation="MSTKSYTSRAETHASPVASKLLRLMDEKKTNLCASLDVRSTDELL
                     KLVETLGPYICLLKTHVDILDDFSYEGTVVPLKALAEKYKFLIFEDRKFADIGNTVKLQ
                     YTSGVYRIAEWSDITNAHGVTGAGIVAGLKQGAQEVTKEPRGLLMLAELSSKGSLAHGE
                     YTKGTVDIAKSDKDFVIGFIAQNDMGGREEGFDWLIMTPGVGLDDKGDALGQQYRTVDE
                     VVSGGSDIIIVGRGLFAKGRDPKVEGERYRNAGWEAYQKRISAPH"
     gene            1412..2215
                     /gene="URA3"
                     /label=URA3
     terminator      2511..2708
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(2813..2831)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3089..3677)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3851..4708)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4709..4813)
                     /label=AmpR promoter
     promoter        5159..5175
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 acagtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
      121 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
      181 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
      241 tcactacttt cacttatggt gttcaatgct tttcaagata cccagatcat atgaaacggc
      301 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
      361 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta
      421 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
      481 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
      541 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc
      601 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
      661 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc
      721 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaatagggcg
      781 cgcctctctg cttttgtgcg cgtatgttta tgtatgtacc tctctctcta tttctatttt
      841 taaaccaccc tctcaataaa ataaaaataa taaagtattt ttaaggaaaa gacgtgttta
      901 agcactgact ttatctactt tttgtacgtt ttcattgata taatgtgttt tgtctctccc
      961 ttttctacga aaatttcaaa aattgaccaa aaaaaggaat atatatacga aaaactatta
     1021 tatttatata tcatagtgtt gataaaaaat gtttatcaga tctcggagac aatcatatgg
     1081 gagaagcaat tggaagatag aaaaaaggta ctcggtacat aaatatatgt gattctgggt
     1141 agaagatcgg tctgcattgg atggtggtaa cgcatttttt tacacacatt acttgcctcg
     1201 agcatcaaat ggtggttatt cgtggatcta tatcacgtga tttgcttaag aattgtcgtt
     1261 catggtgaca cttttagctt tgacatgatt aagctcatct caattgatgt tatctaaagt
     1321 catttcaact atctaagatg tggttgtgat tgggccattt tgtgaaagcc agtacgccag
     1381 cgtcaataca ctcccgtcaa ttagttgcac catgtccaca aaatcatata ccagtagagc
     1441 tgagactcat gcaagtccgg ttgcatcgaa acttttacgt ttaatggatg aaaagaagac
     1501 caatttgtgt gcttctcttg acgttcgttc gactgatgag ctattgaaac ttgttgaaac
     1561 gttgggtcca tacatttgcc ttttgaaaac acacgttgat atcttggatg atttcagtta
     1621 tgagggtact gtcgttccat tgaaagcatt ggcagagaaa tacaagttct tgatatttga
     1681 ggacagaaaa ttcgccgata tcggtaacac agtcaaatta caatatacat cgggcgttta
     1741 ccgtatcgca gaatggtctg atatcaccaa cgcccacggg gttactggtg ctggtattgt
     1801 tgctggcttg aaacaaggtg cgcaagaggt caccaaagaa ccaaggggat tattgatgct
     1861 tgctgaattg tcttccaagg gttctctagc acacggtgaa tatactaagg gtaccgttga
     1921 tattgcaaag agtgataaag atttcgttat tgggttcatt gctcagaacg atatgggagg
     1981 aagagaagaa gggtttgatt ggctaatcat gaccccaggt gtaggtttag acgacaaagg
     2041 cgatgcattg ggtcagcagt acagaaccgt cgacgaagtt gtaagtggtg gatcagatat
     2101 catcattgtt ggcagaggac ttttcgccaa gggtagagat cctaaggttg aaggtgaaag
     2161 atacagaaat gctggatggg aagcgtacca aaagagaatc agcgctcccc attaattata
     2221 caggaaactt aatagaacaa atcacatatt taatctaata gccacctgca ttggcacggt
     2281 gcaacactca cttcaacttc atcttacaaa agatcacgtg atctgttgta ttgaactgaa
     2341 aattttttgt ttgcttctct ctctctcttt cattatgtga gagtttaaaa accagaaact
     2401 acatcatcga aaaagagttt aaaccattac aaccattgcg ataagccctc tcaaacttcc
     2461 tccagaacca atgacgttcc ctcaaccaaa ggtgttttga tgtgaagagt actgacaata
     2521 aaaagattct tgttttcaag aacttgtcat ttgtatagtt tttttatatt gtagttgttc
     2581 tattttaatc aaatgttagc gtgatttata ttttttttcg cctcgacatc atctgcccag
     2641 atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg
     2701 ctatactgct gtcgattcga tactaacgcc gccatccagt ttaaacgagc tcgaattcat
     2761 cgatgatatc agatccacta gtggcctatg cggccgcgga tctgccggtc tccctatagt
     2821 gagtcgtatt aatttcgata agccaggtta acctgcatta atgaatcggc caacgcgcgg
     2881 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2941 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     3001 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3061 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3121 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3181 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3241 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
     3301 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3361 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3421 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3481 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     3541 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3601 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3661 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3721 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3781 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3841 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3901 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3961 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4021 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4081 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4141 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4201 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4261 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4321 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4381 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4441 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4501 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4561 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4621 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4681 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4741 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4801 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     4861 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     4921 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     4981 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     5041 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatggaca
     5101 tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat
     5161 ttaggtgaca ctata
//
