LOCUS       V014966                 5057 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014966
VERSION     V014966
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        132..475
                     /label="TEF promoter"
                     /note="Ashbya gossypii TEF promoter"
     CDS             736..1716
                     /gene="arg3"
                     /label="Ornithine carbamoyltransferase, mitochondrial"
                     /note="Ornithine carbamoyltransferase, mitochondrial from
                     Schizosaccharomyces pombe (strain 972 / ATCC 24843).
                     Accession#: P31317"
     terminator      2407..2604
                     /label="TEF terminator"
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(2712..2730)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2988..3576)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3750..4607)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4608..4712)
                     /label="AmpR promoter"
ORIGIN
        1 atttaggtga cactatagaa cgcggccgcc agctgaagct tcgtacgctg caggtcgacg
       61 gatccccggg ttaattaagg cgcgccagat ctgtttagct tgcctcgtcc ccgccgggtc
      121 acccggccag cgacatggag gcccagaata ccctccttga cagtcttgac gtgcgcagct
      181 caggggcatg atgtgactgt cgcccgtaca tttagcccat acatccccat gtataatcat
      241 ttgcatccat acattttgat ggccgcacgg cgcgaagcaa aaattacggc tcctcgctgc
      301 agacctgcga gcagggaaac gctcccctca cagacgcgtt gaattgtccc cacgccgcgc
      361 ccctgtagag aaatataaaa ggttaggatt tgccactgag gttcttcttt catatacttc
      421 cttttaaaat cttgctagga tacagttctc acatcacatc cgaacataaa caaccatggc
      481 tagacgtact agcttgtttg caactgaact ctagtgtacg ataatgtaaa aattttccat
      541 ctcgtcgaaa atccgcatac gatatattac ggcgccaatc tcgcagtctg agagagaact
      601 agatatcggc tatatgcaat ctcaccctga atatctaaga aattaccagt aacttgtggg
      661 ttccttgtag tcgactacaa aaaccgtatt tttgatatat ttagtcgaga agaattattc
      721 agtttgcaat tgaaaatgtc tttcaaaaaa tttcctcgtc atttactatc cattcgtgat
      781 cttagtcgcg gagagattgt aaagttgatc gatcggtctt ctgaaattaa acaagcttat
      841 aagcagaatt ttcagaatcg ccgttctgtt caaatgagtg gtttgtcgtc tcagaatgtc
      901 gccatgatct tcagtaagcg tagcactcgt actcgtgtat cagtcgaaag tgctgtcagt
      961 tgccttggcg gcaatgccat gtttttaggt aaagatgata tccaattagg agtgaatgag
     1021 tctttatacg atacttcaaa agttatatca agtatggttt ctggtattgt tgctcgcgta
     1081 aacaaatatt cagatgttgc tacgctcgct aaacatgcct cttgtcctgt tattaatggt
     1141 ctttgtgaca cgtttcatcc tctccaggcg ttagcagatt tactcactat taaggaaact
     1201 ttcaaaagct ttgacggttt aaaagttgca tgggtaggtg atgccaataa cgttttacat
     1261 gatttgatga ttgcaaatgc caaggttggt attcatacta gcgttgctaa gcccaaggac
     1321 gtcaacgttc gcgacgatat tttatcaatc gttaacgaag ctgcgaacga aaacggttct
     1381 acttttgaaa ttgttaacga ccccaaggtt gctgttaaaa atgctgatat cgtcgttacc
     1441 gacacttgga tttctatggg tcaagaagct gagaaggaac aacgtcttaa acaatttaca
     1501 ggattccaag tcaccggaga gatcatgaag cttgccaaac ctagctgcaa attcatgcat
     1561 tgtttacccc gtcatcccga ggaagtgtct gacgaggtat tttatggaga gaacagttta
     1621 gttttccaag aagcagagaa ccgcaaatgg accactgttg ctgtcttgga ggctttgctt
     1681 gtcaaccgtg gtgaaattct tcctcctgcc tcagcctaaa ctgaaatcct taatttcatt
     1741 tttatctggg gaatgtgcaa acaatgccaa ctttataatt ttttttattg ggcgtgcaaa
     1801 ttgtgatatt catttttgaa attttatgta ctttgtttca caaaaagaaa attgactttt
     1861 gagaaaattt tttaattgac aattactttt cagatttttt atgcgcagga cacaggcttc
     1921 taaagacgta cttatacgtt aaaaaagggg atggatcaat ttggctcatt agtccaacaa
     1981 gctttgtgta aaattattac ccgcttaatg ctatcctctt aactagatat ttcaaggtat
     2041 caagcaatca tagtattaag cactattata tgcaaaatac aatttaaaat taatagaaac
     2101 tacaaataag cgcaacactc ataaggaaaa agctttggga caataaagct ttcgtcaatg
     2161 cggaatctac gacagaaaga aaacttagtg taggcatcga aatttattat atttcaagca
     2221 tgtatgctag taaaataaaa cgtaaactca ttgattcaag aaatctatag aaaaaaaaga
     2281 tctgggcaag tagagtgtgc atcatcgaaa tccggcttct ttcataaact tatcgtattg
     2341 ctctttaagt gctttgcttt tatcaataaa tggctttttc ttgtcttcct ctagccccta
     2401 atcagtactg acaataaaaa gattcttgtt ttcaagaact tgtcatttgt atagtttttt
     2461 tatattgtag ttgttctatt ttaatcaaat gttagcgtga tttatatttt ttttcgcctc
     2521 gacatcatct gcccagatgc gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt
     2581 atgtgaatgc tggtcgctat actgctgtcg attcgatact aacgccgcca tccagtgtcg
     2641 aaaacgagct cgaattcatc gatgatatca gatccactag tggcctatgc ggccgcggat
     2701 ctgccggtct ccctatagtg agtcgtatta atttcgataa gccaggttaa cctgcattaa
     2761 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2821 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2881 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2941 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3001 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3061 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3121 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3181 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3241 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3301 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3361 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3421 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3481 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3541 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3601 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3661 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3721 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     4021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     4081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     4141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     4201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4381 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     4741 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     4801 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     4861 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     4921 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     4981 agagtgcacc atatggacat attgtcgtta gaacgcggct acaattaata cataacctta
     5041 tgtatcatac acatacg
//
