LOCUS       40924_19216        4312 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Tagging vector pFA6a-5FLAG-kanMX6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Noguchi C, Garabedian MV, Malik M, Noguchi E.
  TITLE     A vector system for genomic FLAG epitope-tagging in 
            Schizosaccharomyces pombe
  JOURNAL   Biotechnol J 3 (9-10), 1280-1285 (2008)
  PUBMED    18729046
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Noguchi C, Garabedian M, Malik M, Noguchi E.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2008) Biochemistry and Molecular Biology, Drexel 
            University College of Medicine, 245 N 15th Street, MS 497, 
            Philadelphia, PA 19102, USA
REFERENCE   3  (bases 1 to 4312)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4312)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J 3 (9-10), 1280-1285 (2008)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-FEB-2008) Biochemistry and Molecular Biology, Drexel University 
            College of Medicine, 245 N 15th Street, MS 497, Philadelphia, PA 
            19102, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             69..200
                     /codon_start=1
                     /label=5xFLAG
                     /note="five tandem FLAG(R) epitope tags"
                     /translation="DYKDDDDKDYKDDDDKLMDYKDDDDKDYKDDDDKLMDYKDDDDK"
     terminator      227..414
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     gene            489..1845
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     promoter        complement(1950..1968)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2226..2814)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2988..3845)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3846..3950)
                     /label=AmpR promoter
     promoter        4296..4312
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 atcatatgga ctacaaggac gacgatgaca aggattacaa agatgacgac gataagctta
      121 tggactacaa ggacgacgat gacaaggatt acaaagatga cgacgataag cttatggact
      181 acaaggacga cgatgacaag catatgggcg cgccacttct aaataagcga atttcttatg
      241 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
      301 tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag
      361 gttgctttct caggtatagt atgaggtcgc tcttattgac cacacctcta ccggcagatc
      421 cgctagggat aacagggtaa tatagatctg tttagcttgc ctcgtccccg ccgggtcacc
      481 cggccagcga catggaggcc cagaataccc tccttgacag tcttgacgtg cgcagctcag
      541 gggcatgatg tgactgtcgc ccgtacattt agcccataca tccccatgta taatcatttg
      601 catccataca ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga
      661 cctgcgagca gggaaacgct cccctcacag acgcgttgaa ttgtccccac gccgcgcccc
      721 tgtagagaaa tataaaaggt taggatttgc cactgaggtt cttctttcat atacttcctt
      781 ttaaaatctt gctaggatac agttctcaca tcacatccga acataaacaa ccatgggtaa
      841 ggaaaagact cacgtttcga ggccgcgatt aaattccaac atggatgctg atttatatgg
      901 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg
      961 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     1021 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     1081 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggcaaaac
     1141 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     1201 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     1261 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     1321 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct
     1381 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     1441 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     1501 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     1561 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     1621 gatgctcgat gagtttttct aatcagtact gacaataaaa agattcttgt tttcaagaac
     1681 ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa tgttagcgtg
     1741 atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa
     1801 gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc gattcgatac
     1861 taacgccgcc atccagttta aacgagctcg aattcatcga tgatatcaga tccactagtg
     1921 gcctatgcgg ccgcggatct gccggtctcc ctatagtgag tcgtattaat ttcgataagc
     1981 caggttaacc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     2041 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2101 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2161 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2221 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2281 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2341 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2401 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2461 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2521 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     2581 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2641 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     2701 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2761 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2821 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2881 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2941 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3001 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3061 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3121 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3181 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3241 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3301 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3361 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3421 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3481 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     3541 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3601 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3661 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3721 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3781 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3841 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3901 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     3961 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     4021 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     4081 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     4141 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     4201 tcagagcaga ttgtactgag agtgcaccat atggacatat tgtcgttaga acgcggctac
     4261 aattaataca taaccttatg tatcatacac atacgattta ggtgacacta ta
//
