LOCUS       40924_19191        4092 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid with a HIS3MX6 marker for adding a C-terminal triple-HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 4092)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4092)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             69..158
                     /codon_start=1
                     /label=3xHA
                     /note="three tandem HA epitope tags"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      191..378
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     gene            425..1625
                     /label=HIS3MX6
                     /note="yeast selectable marker encoding the S. pombe his5
                     gene, which corresponds to S. cerevisiae HIS3"
     promoter        complement(1730..1748)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2006..2594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2768..3625)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3626..3730)
                     /label=AmpR promoter
     promoter        4076..4092
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 acatctttta cccatacgat gttcctgact atgcgggcta tccgtatgac gtcccggact
      121 atgcaggatc ctatccatat gacgttccag attacgctgc tcagtgctga ggcgcgccac
      181 ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa
      241 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt
      301 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat
      361 tgaccacacc tctaccggca gatctgttta gcttgcctcg tccccgccgg gtcacccggc
      421 cagcgacatg gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc
      481 atgatgtgac tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc
      541 catacatttt gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg
      601 cgagcaggga aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta
      661 gagaaatata aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa
      721 aatcttgcta ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaggagg
      781 gcttttgtag aaagaaatac gaacgaaacg aaaatcagcg ttgccatcgc tttggacaaa
      841 gctcccttac ctgaagagtc gaattttatt gatgaactta taacttccaa gcatgcaaac
      901 caaaagggag aacaagtaat ccaagtagac acgggaattg gattcttgga tcacatgtat
      961 catgcactgg ctaaacatgc aggctggagc ttacgacttt actcaagagg tgatttaatc
     1021 atcgatgatc atcacactgc agaagatact gctattgcac ttggtattgc attcaagcag
     1081 gctatgggta actttgccgg cgttaaaaga tttggacatg cttattgtcc acttgacgaa
     1141 gctctttcta gaagcgtagt tgacttgtcg ggacggccct atgctgttat cgatttggga
     1201 ttaaagcgtg aaaaggttgg ggaattgtcc tgtgaaatga tccctcactt actatattcc
     1261 ttttcggtag cagctggaat tactttgcat gttacctgct tatatggtag taatgaccat
     1321 catcgtgctg aaagcgcttt taaatctctg gctgttgcca tgcgcgcggc tactagtctt
     1381 actggaagtt ctgaagtccc aagcacgaag ggagtgttgt aaaggatact gacaataaaa
     1441 agattcttgt tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat
     1501 tttaatcaaa tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg
     1561 cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta
     1621 tactgctgtc gattcgatac taacgccgcc atccagttta aacgagctcg aattcatcga
     1681 tgatatcaga tccactagtg gcctatgcgg ccgcggatct gccggtctcc ctatagtgag
     1741 tcgtattaat ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga
     1801 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1861 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1921 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1981 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2041 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2101 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2161 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     2221 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2281 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2341 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2401 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     2461 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2521 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2581 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2641 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2701 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     2761 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2821 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2881 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2941 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     3001 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     3061 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     3121 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     3181 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     3241 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     3301 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     3361 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     3421 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3481 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3541 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3601 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3661 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3721 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     3781 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     3841 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     3901 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     3961 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat
     4021 tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac atacgattta
     4081 ggtgacacta ta
//
