LOCUS       40924_19181        4675 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid with a kanMX marker for adding a C-terminal 13xMyc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 4675)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4675)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             66..95
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     terminator      618..805
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     gene            852..2208
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     promoter        complement(2313..2331)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2589..3177)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3351..4208)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4209..4313)
                     /label=AmpR promoter
     promoter        4659..4675
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 acggtgaaca aaagctaatc tccgaggaag acttgaacgg tgaacaaaaa ttaatctcag
      121 aagaagactt gaacggactc gacggtgaac aaaagttgat ttctgaagaa gatttgaacg
      181 gtgaacaaaa gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag
      241 aagacttgaa cggactcgac ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg
      301 aacaaaagct aatctccgag gaagacttga acggtgaaca aaaattaatc tcagaagaag
      361 acttgaacgg actcgacggt gaacaaaagt tgatttctga agaagatttg aacggtgaac
      421 aaaagctaat ctccgaggaa gacttgaacg gtgaacaaaa attaatctca gaagaagact
      481 tgaacggact cgacggtgaa caaaagttga tttctgaaga agatttgaac ggtgaacaaa
      541 agctaatctc cgaggaagac ttgaacggtg aacaaaaatt aatcaatcac tagtgaattc
      601 gcgccacttc taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta
      661 taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct
      721 tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag catgaggtcg
      781 ctcttattga ccacacctct accggcagat ctgtttagct tgcctcgtcc ccgccgggtc
      841 acccggccag cgacatggag gcccagaata ccctccttga cagtcttgac gtgcgcagct
      901 caggggcatg atgtgactgt cgcccgtaca tttagcccat acatccccat gtataatcat
      961 ttgcatccat acattttgat ggccgcacgg cgcgaagcaa aaattacggc tcctcgctgc
     1021 agacctgcga gcagggaaac gctcccctca cagacgcgtt gaattgtccc cacgccgcgc
     1081 ccctgtagag aaatataaaa ggttaggatt tgccactgag gttcttcttt catatacttc
     1141 cttttaaaat cttgctagga tacagttctc acatcacatc cgaacataaa caaccatggg
     1201 taaggaaaag actcacgttt cgaggccgcg attaaattcc aacatggatg ctgatttata
     1261 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta
     1321 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     1381 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     1441 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggcaa
     1501 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     1561 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     1621 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     1681 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     1741 gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     1801 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     1861 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     1921 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     1981 tttgatgctc gatgagtttt tctaatcagt actgacaata aaaagattct tgttttcaag
     2041 aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc aaatgttagc
     2101 gtgatttata ttttttttcg cctcgacatc atctgcccag atgcgaagtt aagtgcgcag
     2161 aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct gtcgattcga
     2221 tactaacgcc gccatccagt ttaaacgagc tcgaattcat cgatgatatc agatccacta
     2281 gtggcctatg cggccgcgga tctgccggtc tccctatagt gagtcgtatt aatttcgata
     2341 agccaggtta acctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     2401 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     2461 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     2521 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     2581 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2641 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2701 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2761 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     2821 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2881 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2941 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3001 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     3061 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3121 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3181 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3241 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3301 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3361 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3421 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     3481 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     3541 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     3601 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     3661 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     3721 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     3781 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     3841 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     3901 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     3961 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4021 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4081 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4141 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4201 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4261 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4321 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     4381 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     4441 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     4501 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     4561 gcatcagagc agattgtact gagagtgcac catatggaca tattgtcgtt agaacgcggc
     4621 tacaattaat acataacctt atgtatcata cacatacgat ttaggtgaca ctata
//
