LOCUS       40924_19186        4152 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid with a TRP1 marker for adding a C-terminal 13xMyc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 4152)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4152)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             66..95
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     terminator      618..805
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        813..960
                     /label=TRP1 promoter
     CDS             961..1632
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(1790..1808)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2066..2654)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2828..3685)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3686..3790)
                     /label=AmpR promoter
     promoter        4136..4152
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 acggtgaaca aaagctaatc tccgaggaag acttgaacgg tgaacaaaaa ttaatctcag
      121 aagaagactt gaacggactc gacggtgaac aaaagttgat ttctgaagaa gatttgaacg
      181 gtgaacaaaa gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag
      241 aagacttgaa cggactcgac ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg
      301 aacaaaagct aatctccgag gaagacttga acggtgaaca aaaattaatc tcagaagaag
      361 acttgaacgg actcgacggt gaacaaaagt tgatttctga agaagatttg aacggtgaac
      421 aaaagctaat ctccgaggaa gacttgaacg gtgaacaaaa attaatctca gaagaagact
      481 tgaacggact cgacggtgaa caaaagttga tttctgaaga agatttgaac ggtgaacaaa
      541 agctaatctc cgaggaagac ttgaacggtg aacaaaaatt aatcaatcac tagtgaattc
      601 gcgccacttc taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta
      661 taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct
      721 tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag catgaggtcg
      781 ctcttattga ccacacctct accggcagat ctgtacaatc ttgatccgga gcttttcttt
      841 ttttgccgat taagaattaa ttcggtcgaa aaaagaaaag gagagggcca agagggaggg
      901 cattggtgac tattgagcac gtgagtatac gtgattaagc acacaaaggc agcttggagt
      961 atgtctgtta ttaatttcac aggtagttct ggtccattgg tgaaagtttg cggcttgcag
     1021 agcacagagg ccgcagaatg tgctctagat tccgatgctg acttgctggg tattatatgt
     1081 gtgcccaata gaaagagaac aattgacccg gttattgcaa ggaaaatttc aagtcttgta
     1141 aaagcatata aaaatagttc aggcactccg aaatacttgg ttggcgtgtt tcgtaatcaa
     1201 cctaaggagg atgttttggc tctggtcaat gattacggca ttgatatcgt ccaactgcat
     1261 ggagatgagt cgtggcaaga ataccaagag ttcctcggtt tgccagttat taaaagactc
     1321 gtatttccaa aagactgcaa catactactc agtgcagctt cacagaaacc tcattcgttt
     1381 attcccttgt ttgattcaga agcaggtggg acaggtgaac ttttggattg gaactcgatt
     1441 tctgactggg ttggaaggca agagagcccc gaaagcttac attttatgtt agctggtgga
     1501 ctgacgccag aaaatgttgg tgatgcgctt agattaaatg gcgttattgg tgttgatgta
     1561 agcggaggtg tggagacaaa tggtgtaaaa gactctaaca aaatagcaaa tttcgtcaaa
     1621 aatgctaaga aataggttat tactgagtag tatttattta agtattgttt gtgcacttgc
     1681 ctgcggtgtg aaataccgca cagatgcgta aggaggttta aacgagctcg aattcatcga
     1741 tgatatcaga tccactagtg gcctatgcgg ccgcggatct gccggtctcc ctatagtgag
     1801 tcgtattaat ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga
     1861 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1921 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1981 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2041 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2101 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2161 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2221 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     2281 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2341 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2401 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2461 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     2521 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2581 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2641 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2701 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2761 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     2821 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2881 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2941 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3001 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     3061 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     3121 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     3181 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     3241 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     3301 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     3361 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     3421 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     3481 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3541 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3601 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3661 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3721 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3781 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     3841 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     3901 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     3961 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     4021 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat
     4081 tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac atacgattta
     4141 ggtgacacta ta
//
