LOCUS       40924_19176        4519 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid with a HIS3MX6 marker for adding a C-terminal 13xMyc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 4519)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4519)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             66..95
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     terminator      618..805
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     gene            852..2052
                     /label=HIS3MX6
                     /note="yeast selectable marker encoding the S. pombe his5
                     gene, which corresponds to S. cerevisiae HIS3"
     promoter        complement(2157..2175)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2433..3021)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3195..4052)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4053..4157)
                     /label=AmpR promoter
     promoter        4503..4519
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 acggtgaaca aaagctaatc tccgaggaag acttgaacgg tgaacaaaaa ttaatctcag
      121 aagaagactt gaacggactc gacggtgaac aaaagttgat ttctgaagaa gatttgaacg
      181 gtgaacaaaa gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag
      241 aagacttgaa cggactcgac ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg
      301 aacaaaagct aatctccgag gaagacttga acggtgaaca aaaattaatc tcagaagaag
      361 acttgaacgg actcgacggt gaacaaaagt tgatttctga agaagatttg aacggtgaac
      421 aaaagctaat ctccgaggaa gacttgaacg gtgaacaaaa attaatctca gaagaagact
      481 tgaacggact cgacggtgaa caaaagttga tttctgaaga agatttgaac ggtgaacaaa
      541 agctaatctc cgaggaagac ttgaacggtg aacaaaaatt aatcaatcac tagtgaattc
      601 gcgccacttc taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta
      661 taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct
      721 tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag catgaggtcg
      781 ctcttattga ccacacctct accggcagat ctgtttagct tgcctcgtcc ccgccgggtc
      841 acccggccag cgacatggag gcccagaata ccctccttga cagtcttgac gtgcgcagct
      901 caggggcatg atgtgactgt cgcccgtaca tttagcccat acatccccat gtataatcat
      961 ttgcatccat acattttgat ggccgcacgg cgcgaagcaa aaattacggc tcctcgctgc
     1021 agacctgcga gcagggaaac gctcccctca cagacgcgtt gaattgtccc cacgccgcgc
     1081 ccctgtagag aaatataaaa ggttaggatt tgccactgag gttcttcttt catatacttc
     1141 cttttaaaat cttgctagga tacagttctc acatcacatc cgaacataaa caaccatggg
     1201 taggagggct tttgtagaaa gaaatacgaa cgaaacgaaa atcagcgttg ccatcgcttt
     1261 ggacaaagct cccttacctg aagagtcgaa ttttattgat gaacttataa cttccaagca
     1321 tgcaaaccaa aagggagaac aagtaatcca agtagacacg ggaattggat tcttggatca
     1381 catgtatcat gcactggcta aacatgcagg ctggagctta cgactttact caagaggtga
     1441 tttaatcatc gatgatcatc acactgcaga agatactgct attgcacttg gtattgcatt
     1501 caagcaggct atgggtaact ttgccggcgt taaaagattt ggacatgctt attgtccact
     1561 tgacgaagct ctttctagaa gcgtagttga cttgtcggga cggccctatg ctgttatcga
     1621 tttgggatta aagcgtgaaa aggttgggga attgtcctgt gaaatgatcc ctcacttact
     1681 atattccttt tcggtagcag ctggaattac tttgcatgtt acctgcttat atggtagtaa
     1741 tgaccatcat cgtgctgaaa gcgcttttaa atctctggct gttgccatgc gcgcggctac
     1801 tagtcttact ggaagttctg aagtcccaag cacgaaggga gtgttgtaaa ggatactgac
     1861 aataaaaaga ttcttgtttt caagaacttg tcatttgtat agttttttta tattgtagtt
     1921 gttctatttt aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga catcatctgc
     1981 ccagatgcga agttaagtgc gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg
     2041 gtcgctatac tgctgtcgat tcgatactaa cgccgccatc cagtttaaac gagctcgaat
     2101 tcatcgatga tatcagatcc actagtggcc tatgcggccg cggatctgcc ggtctcccta
     2161 tagtgagtcg tattaatttc gataagccag gttaacctgc attaatgaat cggccaacgc
     2221 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     2281 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2341 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2401 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2461 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2521 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2581 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     2641 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2701 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2761 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2821 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     2881 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2941 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3001 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3061 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3121 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3181 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3241 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3301 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3361 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3421 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3481 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3541 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3601 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3661 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3721 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3781 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     3841 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     3901 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     3961 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     4021 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4081 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4141 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     4201 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     4261 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     4321 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     4381 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     4441 gacatattgt cgttagaacg cggctacaat taatacataa ccttatgtat catacacata
     4501 cgatttaggt gacactata
//
