LOCUS       40924_19161       10079 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mariner transposase delivery vector pFA545, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10079)
  AUTHORS   Bae T, Banger AK, Wallace A, Glass EM, Aslund F, Schneewind O, 
            Missiakas DM.
  TITLE     Staphylococcus aureus virulence genes identified by bursa aurealis 
            mutagenesis and nematode killing
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12312-12317 (2004)
  PUBMED    15304642
REFERENCE   2  (bases 1 to 10079)
  AUTHORS   Bae T, Aslund F, Missiakas DM, Schneewind O.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2004) Molecular Genetics and Cell Biology, 
            Committee on Microbiology, University of Chicago, 920 E. 58th St., 
            Chicago, IL 60637, USA
REFERENCE   3  (bases 1 to 10079)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10079)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2004"; volume: "101"; issue: "33"; pages:
            "12312-12317"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUL-2004) Molecular Genetics and Cell Biology, Committee on 
            Microbiology, University of Chicago, 920 E. 58th St., Chicago, IL 
            60637, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1703..1719)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1727..1743
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1751..1781)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1796..1817)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2105..2693)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2867..3724)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3725..3829)
                     /label=AmpR promoter
     promoter        4619..4637
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             4995..6146
                     /codon_start=1
                     /gene="xylR"
                     /product="Xyl repressor"
                     /label=xylR
                     /protein_id="AAU04865.1"
                     /translation="MENNFIVNENEKRVLKQIFNNSNISRTQISKNLELNKATISNILN
                     NLKHKSLVNEVGEGNSTKSGGRKPILLEINQKYGYYISMDLTYDSVELMYNYFDATILK
                     QDSYELNDKNVSSILQILKSNINVSEKYDTLYGLLGISISIHGIVDDEQNIINLPFHKN
                     EKRTFTDELKSFTNVPVVIENEANLSALYEKSLYINSNINNLITLSIHKGIGAGIIINK
                     KLYRGSNGEAGEIGKTLVLESINNNDNKYYKIEDICSQDALIQKINNRLGVTLTFTELI
                     QYYNEGNSIVAHEIKQFINKMTVLIHNLNTQFNPDAIYINCPLINELPNILNEIKEQFS
                     CFSQGSPIQLHLTTNVKQATLLGGTLAIMQKTLNINNIQMNIK"
     gene            4995..6146
                     /gene="xylR"
                     /label=xylR
     gene            6197..7249
                     /gene="tnp"
                     /label=tnp
     CDS             6197..7243
                     /codon_start=1
                     /gene="tnp"
                     /product="mariner transposase"
                     /label=tnp
                     /protein_id="AAU04866.1"
                     /translation="MEKKEFRVLIKYCFLKGKNTVEAKTWLDNEFPDSAPGKSTIIDWY
                     AKFKRGEMSTEDGERSGRPKEVVTDENIKKIHKMILNDRKMKLIEIAEALKISKERVGH
                     IIHQYLDMRKLCAKWVPRELTFDQKQQRVDDSERCLQLLTRNTPEFFRRYVTMDETWLH
                     HYTPESNRQSAEWTATGEPSPKRGKTQKSAGKVMASVFWDAHGIIFIDYLEKGKTINSD
                     YYMALLERLKVEIAAKRPHMKKKKVLFHQDNAPCHKSLRTMAKIHELGFELLPHPPYSP
                     DLAPSDFFLFSDLKRMLAGKKFGCNEEVIAETEAYFEAKPKEYYQNGIKKLEGRYNRCI
                     ALEGNYVE"
     CDS             complement(7822..8280)
                     /codon_start=1
                     /gene="tetD"
                     /product="polypeptide D"
                     /label=tetD
                     /note="tetracycline resistance protein"
                     /protein_id="AAU04867.1"
                     /translation="MMKTIYHVNVATIGNSVIFPGTMSVIVFGYFGGFLVDRKGSLFVF
                     ILGSLSISISFLTIAFFVEFSMWLTTFMFIFVMGELSFTKTVISKIVSSSLSEEEVASG
                     MSLLNFTSFLSEGTGIAIVGGLLSLQLINRKLVLEFINYSSGVYSNIL"
     gene            complement(7822..8280)
                     /gene="tetD"
                     /label=tetD
     CDS             complement(8237..9124)
                     /codon_start=1
                     /gene="tetB"
                     /product="polypeptide B"
                     /label=tetB
                     /note="tetracycline resistance protein"
                     /protein_id="AAU04868.1"
                     /translation="MFSLYKKFKGLFYSVLFWLCILSFFSVLNEMVLNVSLPDIANHFN
                     TTPGITNWVNTAYMLTFSIGTAVYGKLSDYINIKKLLIIGISLSCLGSLIAFIGHNHFF
                     ILIFGRLVQGVGSAAFPSLIMVVVARNITRKKQGKAFGFIGSIVALGEGLGPSIGGIIA
                     HYIHWSYLLILPMITIVTIPFLIKVMVPGKSTKNTLDIVGIVLMSISIICFMLFTTNYN
                     WTFLILFTIFFVIFIKHISRVSNPFINPKLGKNIPFMLGLFSGWANIFYSSWFYINGAL
                     YDENYLSCKCSDNR"
     gene            complement(8237..9124)
                     /gene="tetB"
                     /label=tetB
ORIGIN
        1 cgatcgtcat tgcatcaaaa cttaattttg gatcagatat gcacacagta tgtgcgtcca
       61 accggctatt agagtagccg gttttagaaa aattgtctaa atcgtgattt tccaaatgat
      121 ttgaatgatt tgcatgattg tttttataca taaaaaatcg actccttaat ctcaatttcg
      181 tttaaggaat cgctcaccca aatatatatc ttgatgtata tttaaatatc gtttaatatc
      241 taaatataca agattataaa aacaactcag tgtttttttc tttgaatgat gtcgttcaca
      301 aactttggtc agggcgtgag cgactccttt ttattttgtt attaatataa cactatcaaa
      361 agatttggtc taatcagatc aagtcttttt ttatttaagc atttgtatta tctggtaaac
      421 agttaaaact actaaaacac caagtacata cttacttgtt ataaaattct caaaggcttc
      481 tctgaatttc atttcttcaa ctccttttat tgctttattc caatttcctt attggtcgga
      541 acctacaggg ctttatgtgt tgttgagttg gtactttctt gggattaatc ccaattcaag
      601 tccaaccaac tcgctaacaa gttagctaac acatagccca ttccaaccaa taagttttct
      661 cggcataaat gcatgttcta taagtgcata cctctatctc gtttttgatt cttttgttgt
      721 tcttgttgct gttcctgttc tgaatataat tctggattca tttttttatc taagtttcct
      781 gctaaaccat ttagctttgg ttcatcttct gttaacttac taactcttcc cgcaaaggta
      841 tttcttccta aaactcgttc taactctttt agtaagattt ttaaaagctt taacgctatt
      901 tctatattct ctttaagtgg ctttgcattt tcgtaaagtt ggttaaaatt atcgtctgcg
      961 ttttcaatac gctcaactgc tttatttaat aaatcatctt tctctcgtat ttccttatct
     1021 ttatcatcta aggctctacc agactttata tactcgtaat cttccgaaat atcttgagca
     1081 gcttttatct gtttctgaaa ttcattgaaa tctttttggc ttattacaac atttccagct
     1141 tcttgtatct ctttgctaaa taaaccacct actttttcag tttcttgctc atacggaaca
     1201 tttataggct ttttaagcgt atttaacgat ttttggtact cttgcattaa tttatcgttc
     1261 ttttgcttta tatggtctgt tttttggctc tcacgttcat attcttgctt atgatattct
     1321 gttttttgtt tatactgact tatttgctca tgtttagcat ttgttacttg tcttgattgc
     1381 ccacgttcta aaccatatcc tcgttgttta acatgctcat taaatctatc ttgaaacgct
     1441 gttaaagctt tttattacct acaacttctt tagcacttaa acgaccatca tcaagtattg
     1501 gaacaacgcc ataatgcatt gtggtgtttt ttcgtccatg tgaactgttg catataataa
     1561 attatcttta ccgtattctt gttctaaaaa ctctttagca tattcaaaaa actgctttgt
     1621 atcttctggc gtttgattat caaagaaatc attgtctgat gtaattaaac catcaatgtg
     1681 tttaatcgaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     1741 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     1801 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     1861 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     1921 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1981 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     2041 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     2101 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     2161 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2221 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2281 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2341 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     2401 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2461 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2521 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     2581 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2641 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2701 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     2761 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     2821 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     2881 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2941 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     3001 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     3061 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     3121 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     3181 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     3241 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     3301 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     3361 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     3421 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3481 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3541 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3601 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     3661 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     3721 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     3781 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3841 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     3901 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     3961 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     4021 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     4081 cagagcagat tgtactgaga gtgcaccata tcgacgctct cccttatgcg actcctgcat
     4141 taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc
     4201 atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
     4261 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc
     4321 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc
     4381 gtagaggatc tggctagcga tgaccctgct gattggttcg ctgaccattt ccggggtgcg
     4441 gaacggcgtt accagaaact cagaaggttc gtccaaccaa accgactctg acggcagttt
     4501 acgagagaga tgatagggtc tgcttcagta agccagatgc tacacaatta ggcttgtaca
     4561 tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat
     4621 ttaggtgaca ctatagaata caagcttggg ctgcaggtcg actctagagg atcgggatcg
     4681 atccaagctt agcaacttaa attcacgtga ctataattat gagaaatcga atcataatta
     4741 ttgtttattc agtaatttta tagacttgct taatttagtt attaattttt atggtacatt
     4801 gaattgatct attctcattt ggcattctac gactataact taaatttata ttttttactt
     4861 tataatatat aattgattat agaataatgt tgctcatatc gtttgccaac atctagtact
     4921 caaattacac tatgttacac ttggtaatat taaccgaact tcccctgtcc aaattagata
     4981 agaggtaata ataaatggaa aataatttta tagtaaatga aaatgagaag cgtgtattaa
     5041 aacaaatttt caataacagc aatatttcac gaacacaaat atcgaagaat ttagaactta
     5101 ataaagctac tatttctaac attctgaaca acttaaaaca caagagttta gttaatgaag
     5161 taggagaagg taatagtact aaaagtggtg gacgaaagcc tattttactc gaaattaacc
     5221 aaaaatatgg ctactatatt tctatggatt taacatatga ttccgttgaa ttaatgtaca
     5281 actactttga tgctactata ttaaagcaag attcctacga attaaatgat aaaaatgtaa
     5341 gcagtatatt acaaatttta aaatctaata taaacgtctc agaaaaatat gatacgttat
     5401 atgggttact tggtatatct atatccatac acggtatcgt tgacgatgag caaaacataa
     5461 tcaatcttcc ttttcataaa aatgagaaac gcacatttac cgatgaatta aagtcattca
     5521 caaatgttcc tgtcgttata gaaaatgaag caaatttatc agcgctatat gaaaaaagtt
     5581 tatatattaa ttcaaacata aataatttga ttactttaag tattcacaag ggtataggcg
     5641 ctggcatcat aataaataaa aaactttatc gtggctcaaa tggagaggct ggagagatag
     5701 gtaagacatt ggttttggaa tctataaata acaatgacaa caaatattat aaaatcgaag
     5761 atatatgctc ccaagacgct ttaatacaga aaataaataa taggttgggc gtcacattga
     5821 cgtttacaga actaatccaa tattacaacg aaggaaattc aattgttgct catgaaatta
     5881 aacaatttat taataaaatg acagttctga ttcataattt gaatacacaa tttaacccag
     5941 acgctattta tattaactgt cctttaatta atgaattacc aaatatttta aatgaaatta
     6001 aagagcaatt ctcctgtttt tctcaaggca gtccaattca attacattta actactaatg
     6061 taaaacaagc tactttattg ggtggcactt tagcaataat gcaaaaaaca ttaaatataa
     6121 ataacattca aatgaatatt aaataattac agcagtctga gttataaaat agatatctcg
     6181 gaccggagga ttcaccatgg aaaaaaagga atttcgtgtt ttgataaaat actgttttct
     6241 gaagggaaaa aatacagtgg aagcaaaaac ttggcttgat aatgagtttc cggactctgc
     6301 cccagggaaa tcaacaataa ttgattggta tgcaaaattc aagcgtggtg aaatgagcac
     6361 ggaggacggt gaacgcagtg gacgcccgaa agaggtggtt accgacgaaa acatcaaaaa
     6421 aatccacaaa atgattttga atgaccgtaa aatgaagttg atcgagatag cagaggcctt
     6481 aaagatatca aaggaacgtg ttggtcatat cattcatcaa tatttggata tgcggaagct
     6541 ctgtgcgaaa tgggtgccgc gcgagctcac atttgaccaa aaacaacaac gtgttgatga
     6601 ttctgagcgg tgtttgcagc tgttaactcg taatacaccc gagtttttcc gtcgatatgt
     6661 gacaatggat gaaacatggc tccatcacta cactcctgag tccaatcgac agtcggctga
     6721 gtggacagcg accggtgaac cgtctccgaa gcgtggaaag actcaaaagt ccgctggcaa
     6781 agtaatggcc tctgtttttt gggatgcgca tggaataatt tttatcgatt atcttgagaa
     6841 gggaaaaacc atcaacagtg actattatat ggcgttattg gagcgtttga aggtcgaaat
     6901 cgcggcaaaa cggccccaca tgaagaagaa aaaagtgttg ttccaccaag acaacgcacc
     6961 gtgccacaag tcattgagaa cgatggcaaa aattcatgaa ttgggcttcg aattgcttcc
     7021 ccacccgccg tattctccag atctggcccc cagcgacttt ttcttgttct cagacctcaa
     7081 aaggatgctc gcagggaaaa aatttggctg caatgaagag gtgatcgccg aaactgaggc
     7141 ctattttgag gcaaaaccga aggagtacta ccaaaatggt atcaaaaaat tggaaggtcg
     7201 ttataatcgt tgtatcgctc ttgaagggaa ctatgttgaa taacccgggc tgcagcatgc
     7261 taagtatggt cgtgagcccc tcccaagctc gcggcctttt ttgtaatgaa gaagggggcg
     7321 agctcgaatt cgtctgttct aatttttctt ttgcctgtat aattctgttc gattttttca
     7381 tcaatcaagt tattaaaatt ctgtttatta gcatttacca aatcataatt taagtaagtt
     7441 ttactatggt ctatatcttc attttcataa ttattatttt ctctttgaac atgtttttgt
     7501 atgcccgttg tatttgttcc agatttaact tttgaaactc taacaatgga ataagacata
     7561 taaaaatcac tcctaagcac aaaggtttta tgtgtattta atagtttaag tctaacacac
     7621 tagacttatt tttaataagt cgttaatacg tgtgctctgc gaggcttaaa cctgtttttg
     7681 ctaacgcaaa aaatgagtgg caaaatgcta gccactcata gttctaaacc aaaatataat
     7741 ataactattc aaactgcttt tcagaacgtt ttaaatacaa taatcgtcaa aagacaactt
     7801 aaataataag gttagccatg gctacagaat attactatac actccagaag aataatttat
     7861 aaattccaga actagtttac gattaatcaa ttgtagtgac aataaacctc ctacaattgc
     7921 tatacctgtt ccctctgata aaaaacttgt gaaatttagc aaactcattc cagaagcaac
     7981 ttcttcttca gaaagactac ttgatactat ttttgatata actgttttag taaaagataa
     8041 ttcgcccata acaaatataa acataaaagt agtcaaccac atactaaact caacaaaaaa
     8101 tgcaatagtt aaaaaactta tagagataga caatgatcct aaaataaaaa caaataatga
     8161 tccttttcta tccactaaaa aaccaccaaa ataaccaaaa acaataacac tcatggttcc
     8221 aggaaaaata acactattac ctattgtcgc tacatttaca tgataaatag ttttcatcat
     8281 ataaggcacc attgatataa aaccagctac tatagaaaat attagcccaa ccagaaaaca
     8341 aaccaagcat aaacggaatg tttttcccta gtttaggatt aataaaaggg ttagaaactc
     8401 ttgaaatatg tttaataaaa atcacaaaaa agattgtgaa gagtattaaa aaagtccaat
     8461 tataatttgt cgtaaataac ataaaacata taatacttat agacattaaa acaataccta
     8521 cgatatctaa tgtatttttt gttgatttac caggtaccat tactttaata agaaaaggta
     8581 tagttactat tgtaatcata ggaagtataa gtaggtaaga ccaatgaata taatgtgcta
     8641 ttattccccc tattgaagga cctaaccctt cacctaaagc tacaattgat cctataaaac
     8701 caaaggcttt gccttgtttt tttcttgtaa tatttctagc tacaaccacc ataatcagtg
     8761 aagggaatgc agcagatcct actccttgta ctaacctacc aaaaatcaaa ataaaaaagt
     8821 gattgtgacc aataaaagca atcaatgaac caagacagct caaactaata ccaataatta
     8881 acaatttttt tatatttata taatcagata attttccata tactgctgtt cctatcgaaa
     8941 aagttaacat atatgcagtg tttacccagt ttgtaattcc aggagtagta ttaaaatgat
     9001 ttgcaatatc aggtaaagaa acatttaaaa ccatttcatt taatacacta aaaaatgaaa
     9061 gaatacaaag ccaaaataaa acgctataaa acaaaccttt aaatttttta tataaactaa
     9121 acaattttac ctctttactt gaggtgacta aagtttatag gggtaaatta ttctttcacc
     9181 tcatataaat tccccaaatt tttaaataga cacttcataa aaaaatcctc ctaaattaga
     9241 atagtttaaa tatatcattt ttgttcagta atattaatat gtacactatt tccaaaattt
     9301 aaattcatgt tgccaaaaat cgatttgttt ttgcaattgt ttttcgttcg cttttaaagt
     9361 cgatttcatt aagtccgtta aatcgactgg cgaaatttct tttatcaaat tcttatattt
     9421 tgttctagaa tttctgtgaa gctttcccca ttcttcttca tcactcaata acataaaaac
     9481 tattgctcta tccgcagtgc gttggatagt tttccaatct ggttgcaaga tatgtaaatc
     9541 actaaagcaa tcattccagt aatccaccat atctctttta agttcgattt ctacacgcca
     9601 taaatgttca gacataactt cagcatctgc attatcttta cgttcttgct ttttattata
     9661 aattctaata aatctattac tatctctcac gccaaaatat tttgtttctg gcttaccatt
     9721 acgaccataa aaaatagttt tcttaactgc tttatcagac attgcatagt agtcactcaa
     9781 atcttcttca aaatcaaagg ctaaatctaa tcttgtaaaa ccgtcatctt ccatgtagct
     9841 tattatattt tgttttaacc aaatcatttc atctcgtgta agtttgtttg gattaaattc
     9901 aatacgcata ttacgtctat cccaactatc tgctttcact ttgtcatatt caatataaac
     9961 tttttcttga agtgctttag ctttaaactt tgtttgaaga atatcccaaa gtcttatttg
    10021 gggctctaca ctcataaatt tagaaagggc ttgagcgttg tctcggttga gatttccaa
//
