LOCUS       V006380                 4803 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006380
VERSION     V006380
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M.
  TITLE     Actin dynamics and endocytosis in the absence of Arp2/3 in Candida
            albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2011) Biology, McGill University, 1205 Docteur
            Penfield, Montreal, Quebec H3A 1B1, Canada
REFERENCE   3  (bases 1 to 4803)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4803)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-MAY-2011) Biology, McGill University, 1205 Docteur Penfield,
            Montreal, Quebec H3A 1B1, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             55..765
                     /label="superecliptic pHluorin"
                     /note="pH-sensitive mutant of green fluorescent protein (Ng
                     et al., 2002)"
     regulatory      769..1024
                     /label="Saccharomyces cerevisiae URA3 terminator"
                     /note="Saccharomyces cerevisiae URA3 terminator"
                     /regulatory_class="terminator"
     CDS             1427..2236
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     promoter        complement(2441..2459)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2717..3305)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3479..4336)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4337..4441)
                     /label="AmpR promoter"
     promoter        4787..4803
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcagggtg ctggcgcagg tgcttctaaa
       61 ggtgaagaat tattcactgg tgttgtccca attttggttg aattagatgg tgatgttaat
      121 ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg ctacttacgg taaattgacc
      181 ttaaaattta tttgtactac tggtaaattg ccagttccat ggccaacctt agtcactact
      241 ttatgttatg gtgttcaatg tttttcaaga tacccagatc atatgaaacg acatgacttt
      301 ttcaagtctg ccatgccaga aggttatgtt caagaaagaa ctattttttt caaagatgac
      361 ggtaactaca agaccagagc tgaagtcaag tttgaaggtg ataccttagt taatagaatc
      421 gaattaaaag gtattgattt taaagaagat ggtaacattt taggtcacaa attggaatac
      481 aactataacg atcaccaagt ttacatcatg gctgacaaac aaaagaatgg tatcaaagct
      541 aacttcaaaa ttagacacaa cattgaagat ggtggtgttc aattagctga ccattatcaa
      601 caaaatactc caattggtga tggtccagtc ttgttaccag acaaccatta cttattcact
      661 acatctacct tatccaaaga tccaaacgaa aagagagacc acatggtctt gttagaattt
      721 gttactgctg ctggtattac ccatggtatg gatgaattgt acaaataagg cgcgccatta
      781 taagtaaatg catgtatact aaactcacaa attagagctt caatttaatt atatcagtta
      841 ttacccggga atctcggtcg taatgatttc tataatgacg aaaaaaaaaa aattggaaag
      901 aaaaagcttc atggccttta taaaaaggaa ctatccaata cctcgccaga accaagtaac
      961 agtattttac ggggcacaaa tcaagaacaa taagacagga ctgtaaagat ggacgcatag
     1021 atccggatgg tataaacgga aacaaaaaaa agagctggta ctactttctt taaaattatt
     1081 ttattatttg attttattta atagtatata ttatattttg aacgtagatt attttgttga
     1141 aagttgctgt agtgccattg attcgtaaca ctaattctgt attagtcatt cctcttgttt
     1201 gatagtatcc aaaaaaacgg ctattttttt gcaatcttat ttcctgcata ttatacagat
     1261 aacataatga aagaaaaaat cttttttttt gttcttcaat gatgatttca accattcttt
     1321 taaacattga tcaattcctg agcaacaacc ccatacacac tggtttatat accgcccctt
     1381 ttacagttga agaaagaaat agaaatagaa atagcaaaca aaagatatga cagtcaacac
     1441 taagacctat agtgagagag cagaaactca tgcctcacca gtagcacagc gattatttcg
     1501 attaatggaa ctgaagaaaa ccaatttatg tgcatcaatt gacgttgata ccactaagga
     1561 attccttgaa ttaattgata aattaggtcc ttatgtatgc ttaatcaaga ctcatattga
     1621 tataatcaat gatttttcct atgaatccac tattgaacca ttattagaac tttcacgtaa
     1681 acatcaattt atgatttttg aagatagaaa atttgctgat attggtaata ccgtaaagaa
     1741 acaatatatt ggtggagttt ataaaattag tagttgggca gatattacca atgctcatgg
     1801 tgtcactggg aatggagtgg ttgaaggatt aaaacaggga gctaaagaaa ccaccaccaa
     1861 ccaagagcca agagggttat tgatgttagc tgaattatca tcagtgggat cattagcata
     1921 tggagaatat tctcaaaaaa ctgttgaaat tgctaaatcc gataaggaat ttgttattgg
     1981 atttattgcc caacgtgata tgggtggcca agaagaagga tttgattggc ttattatgac
     2041 acctggagtt ggattagatg ataaaggtga tggattagga caacaatata gaactgttga
     2101 tgaagttgtt agcactggaa ctgatattat cattgttggt agaggattgt ttggtaaagg
     2161 aagagatcca gatattgaag gtaaaaggta tagaaatgct ggttggaatg cttatttgaa
     2221 aaagactggc caattataaa tgtgaagggg gagattttca ctttattaga tttgtatata
     2281 tgtagaataa ataaataaat aagttaaata aataattaaa taagggtggt aattattact
     2341 atttacaatc aaaggtggtc cttctagttt aaacgagctc gaattcatcg atgatatcag
     2401 atccactagt ggcctatgcg gccgcggatc tgccggtctc cctatagtga gtcgtattaa
     2461 tttcgataag ccaggttaac ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2521 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     2581 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     2641 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2701 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2761 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2821 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2881 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     2941 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3001 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3061 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3121 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     3181 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3241 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3301 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3361 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3421 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3481 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3541 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3601 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3661 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3721 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3781 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3841 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3901 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3961 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4021 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4081 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4141 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4201 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4261 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4321 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4381 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4441 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     4501 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     4561 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     4621 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     4681 actatgcggc atcagagcag attgtactga gagtgcacca tatggacata ttgtcgttag
     4741 aacgcggcta caattaatac ataaccttat gtatcataca catacgattt aggtgacact
     4801 ata
//
