LOCUS       40924_19121        5412 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFA-pHluorin-ARG4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M.
  TITLE     Actin dynamics and endocytosis in the absence of Arp2/3 in Candida 
            albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2011) Biology, McGill University, 1205 Docteur 
            Penfield, Montreal, Quebec H3A 1B1, Canada
REFERENCE   3  (bases 1 to 5412)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5412)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAY-2011) Biology, McGill University, 1205 Docteur Penfield, 
            Montreal, Quebec H3A 1B1, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             55..765
                     /label=superecliptic pHluorin
                     /note="pH-sensitive mutant of green fluorescent protein (Ng
                     et al., 2002)"
     regulatory      771..1024
                     /label=Saccharomyces cerevisiae URA3 terminator
                     /note="Saccharomyces cerevisiae URA3 terminator"
                     /regulatory_class="terminator"
     CDS             1482..2888
                     /codon_start=1
                     /gene="ARG4"
                     /product="Arg4"
                     /label=ARG4
                     /protein_id="AEI30145.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELNLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDALGGTAKSAVLK
                     QLENLKSILS"
     gene            1482..2888
                     /gene="ARG4"
                     /label=ARG4
                     /note="selectable marker ARG4 from C. albicans"
     promoter        complement(3050..3068)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3326..3914)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4088..4945)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4946..5050)
                     /label=AmpR promoter
     promoter        5396..5412
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcagggtg ctggcgcagg tgcttctaaa
       61 ggtgaagaat tattcactgg tgttgtccca attttggttg aattagatgg tgatgttaat
      121 ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg ctacttacgg taaattgacc
      181 ttaaaattta tttgtactac tggtaaattg ccagttccat ggccaacctt agtcactact
      241 ttatgttatg gtgttcaatg tttttcaaga tacccagatc atatgaaacg acatgacttt
      301 ttcaagtctg ccatgccaga aggttatgtt caagaaagaa ctattttttt caaagatgac
      361 ggtaactaca agaccagagc tgaagtcaag tttgaaggtg ataccttagt taatagaatc
      421 gaattaaaag gtattgattt taaagaagat ggtaacattt taggtcacaa attggaatac
      481 aactataacg atcaccaagt ttacatcatg gctgacaaac aaaagaatgg tatcaaagct
      541 aacttcaaaa ttagacacaa cattgaagat ggtggtgttc aattagctga ccattatcaa
      601 caaaatactc caattggtga tggtccagtc ttgttaccag acaaccatta cttattcact
      661 acatctacct tatccaaaga tccaaacgaa aagagagacc acatggtctt gttagaattt
      721 gttactgctg ctggtattac ccatggtatg gatgaattgt acaaataagg cgcgccatta
      781 taagtaaatg catgtatact aaactcacaa attagagctt caatttaatt atatcagtta
      841 ttacccggga atctcggtcg taatgatttc tataatgacg aaaaaaaaaa aattggaaag
      901 aaaaagcttc atggccttta taaaaaggaa ctatccaata cctcgccaga accaagtaac
      961 agtattttac ggggcacaaa tcaagaacaa taagacagga ctgtaaagat ggacgcatag
     1021 atcccccctt tagtaagatt tttcaagagt agtctcaaat aaacagaatg aaggattgaa
     1081 ggttttattc aataaataat tggacagaaa gttattgcac agttttaaca acaacaattt
     1141 caaaatttca atttatgaca tattgaccga cataattcag tcatttctaa tattcaaata
     1201 acaagacacc ttgtataaat gaatccacaa gcgtatatga acaaccaaat tttatgacta
     1261 actgtgggtc gtgtactttt gacgattgag tcatttttac caattaggta attgaatttt
     1321 actaatttgt cattaaccgc gctggccatc ccaacaacaa caacaacaat caatttaaaa
     1381 acttgacaag gcaaaaaaaa aaaatttcat ctttgaaaaa tttttcttcc ttttcttctt
     1441 ctgttcttta cataacagct atcaagaata atcaattaat aatgtcacaa caacaagata
     1501 aacaacctag tgaaaataaa ttatggggtg gtcgtttcac cggtgccact gatccattga
     1561 tggatttgta taatgcttca ttaccttatg ataaagttat gtatgatgct gatttaactg
     1621 gtactaaagt atacacacaa ggattaaaca aattaggatt aattaccact gaagaattga
     1681 atttgattca tcaaggatta gaacaaatca gacaagaatg gcatgataat aaattcatta
     1741 ttaaggctgg tgatgaggat attcatactg ctaatgaacg tcgattagga gaaatcattg
     1801 ggaaaaacat ttctggtaaa gttcacactg gtagatcaag aaatgatcaa gttgctaccg
     1861 atatgagaat tttcgttcgt gaatcattac ttaatttaag taaaatttta catcaattca
     1921 tcacggcaat tcttgaacga gcacataaag aaatcgatgt attaatgcct ggttatactc
     1981 atttacaaaa agctcaacct attagatggg cccattggtt aagttcatat gcaacttatt
     2041 tcaccgaaga ttataaacgt ttacaagaaa ttataactcg tgttaaccaa tctccattag
     2101 gatctggtgc tttagcaggt catccttatg gtattgatcg tgaattttta gctaaaggat
     2161 taggatttga tggtgtcatt gggaattctt taactgcagt ttcagatcgt gattttgttg
     2221 ttgaaagttt attttggagt acattattta tgaatcatat ttctcgattc tcagaagatc
     2281 taattattta ttctagtggt gaatttgggt tcataaaatt agctgatgct tattctactg
     2341 gttcttcatt gatgccacaa aagaaaaacc ctgactcatt agaattatta cgtgggaaaa
     2401 gtggtagagt atttggtcag ttatctggat ttttaatgtc gatcaaatct attccatcta
     2461 cttataataa agatatgcaa gaggataaag aaccattatt tgatgcattg actacagtag
     2521 aacattcaat tttaattgct actggtgtga tttctacttt actgattgat aaacaaaata
     2581 tggaaaaagc tttaactatg gatatgttgg ctactgattt agctgattat ttagttagaa
     2641 aaggagttcc atttagagaa actcatcata tatctggtga atgtgttaga aaagctgaag
     2701 aagaaaaatt atctggaatt gatcaattat cttttgaaca atttcaacaa attgattctc
     2761 gttttgaaaa agacgttatg gaaacttttg attttgaagc tagtgtggaa agaagagatg
     2821 ctcttggtgg tactgctaaa agtgccgttt taaaacaatt agaaaattta aaatcaatct
     2881 taagttaatt tataaatagt catataataa tcacagtatt gtttaactta cataaaatat
     2941 aatcaattaa cacagagata ccttgtacta caatagttta aacgagctcg aattcatcga
     3001 tgatatcaga tccactagtg gcctatgcgg ccgcggatct gccggtctcc ctatagtgag
     3061 tcgtattaat ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga
     3121 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3181 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3241 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3301 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3361 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3421 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3481 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     3541 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3601 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3661 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3721 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     3781 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3841 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3901 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3961 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4021 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4081 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4141 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4201 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4261 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4321 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4381 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4441 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4501 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4561 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4621 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4681 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4741 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4801 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4861 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4921 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4981 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     5041 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     5101 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     5161 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     5221 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     5281 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat
     5341 tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac atacgattta
     5401 ggtgacacta ta
//
