LOCUS       V015397                 7219 bp    DNA     circular SYN 17-JUL-2019
DEFINITION  Exported.
ACCESSION   V015397
VERSION     V015397
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 7219)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 7219)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 13
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE
            (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG),
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez
            2, Salamanca, Salamanca 37007, Spain"
FEATURES             Location/Qualifiers
     source          1..7219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             56..85
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     regulatory      224..479
                     /note="Transcriptional termination from Saccharomyces
                     cerevisiae URA3 gene"
                     /regulatory_class="terminator"
     primer_bind     509..525
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    539..572
                     /label="loxP"
                     /note="loxP"
     CDS             1002..1811
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     regulatory      1956..3287
                     /note="CaMET3 promoter; inducible upon growth without
                     methionine repressible via addition of methionine and
                     cysteine to the medium"
                     /regulatory_class="promoter"
     CDS             join(3315..3718,3883..4510)
                     /codon_start=1
                     /transl_table=11
                     /product="Cre"
                     /label="Cre"
                     /note="Cre recombinase"
                     /protein_id="QDK59788.1"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          3719..3882
                     /note="modified TUB2 intron sequence"
     3'UTR           4523..4653
                     /label="Saccharomyces cerevisiae ADH1 3'UTR region"
                     /note="Saccharomyces cerevisiae ADH1 3'UTR region"
     regulatory      4654..4713
                     /note="Transcriptional termination from Saccharomyces
                     cerevisiae CYC1 gene"
                     /regulatory_class="terminator"
     protein_bind    complement(4726..4759)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(4857..4875)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(5133..5721)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5895..6752)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6753..6857)
                     /label="AmpR promoter"
     promoter        7203..7219
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgag aagcttcgta cgctgcaggt cgacggatcc ccggggaaca
       61 gaagcttata tccgaagaag acctcggaga gcaaaagctc atttcagaag aggatctagg
      121 cgaacagaaa ctaatctcgg aggaggacct cggtgaacaa aagcttatct ctgaggaaga
      181 tcttggcgag cagaagctca tatcagagga agacctaggg tagggcgcgc cattataagt
      241 aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc agttattacc
      301 cgggaatctc ggtcgtaatg atttctataa tgacgaaaaa aaaaaaattg gaaagaaaaa
      361 gcttcatggc ctttataaaa aggaactatc caatacctcg ccagaaccaa gtaacagtat
      421 tttacggggc acaaatcaag aacaataaga caggactgta aagatggacg catagatcct
      481 cgtacgctgc aggtcgacgg gcggccgctc tagaactagt ggatccagat cttgatccat
      541 aacttcgtat agcatacatt atacgaagtt atagatccag tactaatagg aattgatttg
      601 gatggtataa acggaaacaa aaaaaagagc tggtactact ttctttaaaa ttattttatt
      661 atttgatttt atttaatagt atatattata ttttgaacgt agattatttt gttgaaagtt
      721 gctgtagtgc cattgattcg taacactaat tctgtattag tcattcctct tgtttgatag
      781 tatccaaaaa aacggctatt tttttgcaat cttatttcct gcatattata cagataacat
      841 aatgaaagaa aaaatctttt tttttgttct tcaatgatga tttcaaccat tcttttaaac
      901 attgatcaat tcctgagcaa caaccccata cacactggtt tatatatcgc cccttttaca
      961 gttgaagaaa gaaatagaaa tagaaatagc aaacaaaaga tatgacagtc aacactaaga
     1021 cctatagtga gagagcagaa actcatgcct caccagtagc acaacgatta tttcgattaa
     1081 tggaactgaa gaaaaccaat ttatgtgcat caattgatgt tgataccact aaggaattcc
     1141 ttgaattaat tgataaattg ggtccttatg tatgcttaat caagactcat attgatataa
     1201 tcaatgattt ttcctatgaa tccactattg aaccattatt agaactttca cgtaaacatc
     1261 aatttatgat ttttgaagat agaaaatttg ctgatattgg taataccgtg aagaaacaat
     1321 atattggtgg agtttataaa attagtagtt gggcagatat tactaatgct catggtgtca
     1381 ctgggaatgg agtagttgaa ggattaaaac agggagctaa agaaaccacc accaaccaag
     1441 agccaagagg gttattgatg ttagctgaat tatcatcagt gggatcatta gcatatggag
     1501 aatattctca aaaaactgtt gaaattgcta aatccgataa ggaatttgtt attggattta
     1561 ttgcccaacg tgatatgggt ggacaagaag aaggatttga ttggcttatt atgacacctg
     1621 gagttggatt agatgataaa ggtgatggat taggacaaca atatagaact gttgatgaag
     1681 ttgttagcac tggaactgat attatcattg ttggtagagg attgtttggt aaaggaagag
     1741 atccagatat tgaaggtaaa aggtatagag atgctggttg gaatgcttat ttgaaaaaaa
     1801 ctggccaatt ataaatgtga agggggagat tttcacttta ttagatttgt atatatgtat
     1861 aataaataaa taaataagtt aaataaataa ttaaataagg gtggtaatta ttactattta
     1921 caatcaaagg tggtccttct agaagatctg ctagcaattg tctattccaa gcctgtggct
     1981 ttttttggtg attcacaata cataaattgg caaatacctc ttctatagac tttaaccttt
     2041 gatgaaatgg tatggataaa ccacttttgt ggtcagggta tatatatttt tattccaatc
     2101 tggcctttat aaattcaaat ttccaagggg actctgctaa accttctgat ataactataa
     2161 ctctaataat ggctgtaaaa agtgtgaaaa aaagatgata ttcacaaaaa agataaaggg
     2221 gatttcatat tgggtatcta gatttaaaca tcgtatttca acaatgaaac tagaatcaat
     2281 acgaaatcca gcgcacaact gtgttcattc ccttgggaat tatactgagt aatgggaaga
     2341 atgtgcaaaa aatacccgtg tcttatataa tttccatttg tgtagggctt aaaagtggac
     2401 aacttcaggt tacgaaacca cgaaaatgtc taaacgtttg atcaaattcg taactcatgg
     2461 aaaatcacag caacaaatta aggatggata tatccggtac ttacaaataa aaacactaaa
     2521 gaagtataaa ttttgaactg aaattctata gaaaatcttt ttttttgggt aaaattatgc
     2581 gattgtggct catagtaacg taatcataca gttcaacaaa catcattttg tcaaaggatt
     2641 tctaatccca ttctttttct acttggcttt tgagtatata tatatacatc ctttgttcat
     2701 catcatgttc gattgaattt ttttcttggt tgttattaga ttgtctactt gagtttttct
     2761 tggtgcaaat tgtggtgttt ttctaacaaa tttttcaaca tttaaagtat ttctggaact
     2821 tttctacaag gaacgacata gatccattaa tgcgcccctc taaaacaata cccataatgt
     2881 cttcttaagt atgcatctgc ttataaatgt ttctggattt actttagtta gaatgtcgac
     2941 acgacaatga actattgctg tggatcacgt gcgatcactg aattatgagt ttctttttat
     3001 ttttcttttt gggctttttt ttgcaatttt ttttttccac ctttgctaat tgcgtagtca
     3061 catataatgt atacaatttt tgcaactctg acactataat tgatccaata ataaagtata
     3121 taatagatca tcaagtatac gtaatctccc caaaaaaaaa ttgcataaaa taaaatctat
     3181 aaaccttttt tagattttta gttgttcctt ttttctttct attcttcaac ttccaagtgt
     3241 tgtcactttc tccctgttaa ctatatttaa aagtaaatac cctccccccc gggtatactc
     3301 attaatcaat aattatgtct aacttgttga ccgttcacca aaacttgcca gctttgccag
     3361 ttgatgctac ctctgatgaa gttagaaaaa acttgatgga tatgtttaga gatagacaag
     3421 ccttctctga acacacctgg aaaatgttgt tgtctgtttg tagatcctgg gctgcttggt
     3481 gtaaattgaa caacagaaaa tggttcccag ccgaaccaga agatgttaga gattacttgt
     3541 tgtacttgca agctagaggt ttggctgtta aaaccattca acaacacttg ggtcaattga
     3601 acatgttgca cagaagatcc ggtttgccaa gaccatctga ttctaacgct gtttctttgg
     3661 ttatgagaag aattagaaaa gaaaacgttg atgctggtga aagagctaaa caagccttgt
     3721 aggtatagac actgaagaaa aaaaaattct atcattgtta tgttgtgatc tttgatcttt
     3781 agttgtcggg ttaacacctg ccaattggat caatacatca atcaattaat tctaatcttg
     3841 aaaaaaaaat tcattttact aacaattttt tcttttattt agggctttcg aaagaaccga
     3901 tttcgatcaa gttagatcct tgatggaaaa ctctgataga tgtcaagata ttagaaactt
     3961 ggctttcttg ggtattgctt acaacacctt gttgagaatt gctgaaattg ctagaattag
     4021 agttaaagat atttccagaa ccgatggtgg tagaatgttg attcacattg gtagaaccaa
     4081 aaccttggtt tctaccgctg gtgttgaaaa agccttgtct ttgggtgtta ccaaattggt
     4141 tgaaagatgg atttctgttt ctggtgttgc tgatgatcca aacaactact tgttctgtag
     4201 agttagaaaa aacggtgttg ctgctccatc tgctacctct caattatcta ccagagcttt
     4261 ggaaggtatt ttcgaagcta cccacagatt gatttacggt gctaaagatg attctggtca
     4321 aagatacttg gcttggtctg gtcactctgc tagagttggt gctgctagag atatggctag
     4381 agccggcgtt tctattccag aaattatgca agctggtggt tggaccaacg ttaacattgt
     4441 tatgaactac attagaaact tggattctga aaccggtgct atggttagat tgttggaaga
     4501 tggtgattaa tttataccta ggtaagcaaa tagctaaatt atatacgaat taatattatg
     4561 attaagtgtt tacgtgagtg cgatattttt attactatct tatacagttg tatatactct
     4621 ataaaatgag ttgtctatta attaacgcga tgagtcccta tttatttttt tatagttatg
     4681 ttagtattaa gaacgttatt tatatttcaa attaattatc catggataac ttcgtatagc
     4741 atacattata cgaagttatt agggataaca gggtaatctg caggaaaaac gagctcgaat
     4801 tcatcgatga tatcagatcc actagtggcc tatgcggccg cggatctgcc ggtctcccta
     4861 tagtgagtcg tattaatttc gataagccag gttaacctgc attaatgaat cggccaacgc
     4921 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     4981 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     5041 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     5101 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5161 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5221 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     5281 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     5341 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5401 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5461 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     5521 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     5581 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     5641 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5701 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     5761 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     5821 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     5881 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     5941 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     6001 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     6061 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     6121 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     6181 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     6241 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     6301 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     6361 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     6421 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     6481 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     6541 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     6601 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     6661 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     6721 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     6781 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     6841 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     6901 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     6961 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     7021 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     7081 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     7141 gacatattgt cgttagaacg cggctacaat taatacataa ccttatgtat catacacata
     7201 cgatttaggt gacactata
//
