LOCUS       V016463                 4433 bp    DNA     circular SYN 17-JUL-2019
DEFINITION  Exported.
ACCESSION   V016463
VERSION     V016463
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 4433)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 13
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE
            (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG),
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez
            2, Salamanca, Salamanca 37007, Spain"
FEATURES             Location/Qualifiers
     source          1..4433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             54..83
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     regulatory      222..477
                     /note="Transcriptional termination from Saccharomyces
                     cerevisiae URA3 gene"
                     /regulatory_class="terminator"
     primer_bind     487..503
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    517..550
                     /label="loxP"
                     /note="loxP"
     CDS             980..1789
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     protein_bind    complement(1940..1973)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(2071..2089)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2347..2935)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3109..3966)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3967..4071)
                     /label="AmpR promoter"
     promoter        4417..4433
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc ggggaacaga
       61 agcttatatc cgaagaagac ctcggagagc aaaagctcat ttcagaagag gatctaggcg
      121 aacagaaact aatctcggag gaggacctcg gtgaacaaaa gcttatctct gaggaagatc
      181 ttggcgagca gaagctcata tcagaggaag acctagggta gggcgcgcca ttataagtaa
      241 atgcatgtat actaaactca caaattagag cttcaattta attatatcag ttattacccg
      301 ggaatctcgg tcgtaatgat ttctataatg acgaaaaaaa aaaaattgga aagaaaaagc
      361 ttcatggcct ttataaaaag gaactatcca atacctcgcc agaaccaagt aacagtattt
      421 tacggggcac aaatcaagaa caataagaca ggactgtaaa gatggacgca tagatccggc
      481 ggccgctcta gaactagtgg atccagatct tgatccataa cttcgtatag catacattat
      541 acgaagttat agatccagta ctaataggaa ttgatttgga tggtataaac ggaaacaaaa
      601 aaaagagctg gtactacttt ctttaaaatt attttattat ttgattttat ttaatagtat
      661 atattatatt ttgaacgtag attattttgt tgaaagttgc tgtagtgcca ttgattcgta
      721 acactaattc tgtattagtc attcctcttg tttgatagta tccaaaaaaa cggctatttt
      781 tttgcaatct tatttcctgc atattataca gataacataa tgaaagaaaa aatctttttt
      841 tttgttcttc aatgatgatt tcaaccattc ttttaaacat tgatcaattc ctgagcaaca
      901 accccataca cactggttta tatatcgccc cttttacagt tgaagaaaga aatagaaata
      961 gaaatagcaa acaaaagata tgacagtcaa cactaagacc tatagtgaga gagcagaaac
     1021 tcatgcctca ccagtagcac aacgattatt tcgattaatg gaactgaaga aaaccaattt
     1081 atgtgcatca attgatgttg ataccactaa ggaattcctt gaattaattg ataaattggg
     1141 tccttatgta tgcttaatca agactcatat tgatataatc aatgattttt cctatgaatc
     1201 cactattgaa ccattattag aactttcacg taaacatcaa tttatgattt ttgaagatag
     1261 aaaatttgct gatattggta ataccgtgaa gaaacaatat attggtggag tttataaaat
     1321 tagtagttgg gcagatatta ctaatgctca tggtgtcact gggaatggag tagttgaagg
     1381 attaaaacag ggagctaaag aaaccaccac caaccaagag ccaagagggt tattgatgtt
     1441 agctgaatta tcatcagtgg gatcattagc atatggagaa tattctcaaa aaactgttga
     1501 aattgctaaa tccgataagg aatttgttat tggatttatt gcccaacgtg atatgggtgg
     1561 acaagaagaa ggatttgatt ggcttattat gacacctgga gttggattag atgataaagg
     1621 tgatggatta ggacaacaat atagaactgt tgatgaagtt gttagcactg gaactgatat
     1681 tatcattgtt ggtagaggat tgtttggtaa aggaagagat ccagatattg aaggtaaaag
     1741 gtatagagat gctggttgga atgcttattt gaaaaaaact ggccaattat aaatgtgaag
     1801 ggggagattt tcactttatt agatttgtat atatgtataa taaataaata aataagttaa
     1861 ataaataatt aaataagggt ggtaattatt actatttaca atcaaaggtg gtccttctag
     1921 aagatctgct agcccatgga taacttcgta tagcatacat tatacgaagt tattagggat
     1981 aacagggtaa tctgcaggaa aaacgagctc gaattcatcg atgatatcag atccactagt
     2041 ggcctatgcg gccgcggatc tgccggtctc cctatagtga gtcgtattaa tttcgataag
     2101 ccaggttaac ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2161 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2221 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2281 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2341 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2401 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2461 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2521 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2581 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2641 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2701 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2761 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     2821 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2881 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2941 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3001 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3061 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3121 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3181 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3241 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3301 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3361 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3421 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3481 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3541 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3601 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3661 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3721 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3781 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3841 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3901 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     3961 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4021 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4081 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     4141 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     4201 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     4261 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     4321 atcagagcag attgtactga gagtgcacca tatggacata ttgtcgttag aacgcggcta
     4381 caattaatac ataaccttat gtatcataca catacgattt aggtgacact ata
//
