LOCUS       V015738                 4327 bp    DNA     circular SYN 17-JUL-2019
DEFINITION  Exported.
ACCESSION   V015738
VERSION     V015738
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     A new toolkit for gene tagging in Candida albicans containing
            recyclable markers
  JOURNAL   PLoS ONE (2019) In press
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Duenas-Santero E, Santos-Almeida A, Rojo-Dominguez P, del Rey F,
            Correa-Bordes J, Vazquez de Aldana CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Instituto de Biologia Funcional y Genomica
            (IBFG), Consejo Superior de Investigaciones Cientificas, Zacarias
            Gonzalez 2, Salamanca, Salamanca 37007, Spain
REFERENCE   3  (bases 1 to 4327)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 13
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE
            (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAR-2019) Instituto de Biologia Funcional y Genomica (IBFG),
            Consejo Superior de Investigaciones Cientificas, Zacarias Gonzalez
            2, Salamanca, Salamanca 37007, Spain"
FEATURES             Location/Qualifiers
     source          1..4327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             54..83
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     regulatory      222..477
                     /note="Transcriptional termination from Saccharomyces
                     cerevisiae URA3 gene"
                     /regulatory_class="terminator"
     primer_bind     487..503
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    517..550
                     /label="loxP"
                     /note="loxP"
     CDS             898..1791
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     protein_bind    complement(1834..1867)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(1965..1983)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2241..2829)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3003..3860)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3861..3965)
                     /label="AmpR promoter"
     promoter        4311..4327
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc ggggaacaga
       61 agcttatatc cgaagaagac ctcggagagc aaaagctcat ttcagaagag gatctaggcg
      121 aacagaaact aatctcggag gaggacctcg gtgaacaaaa gcttatctct gaggaagatc
      181 ttggcgagca gaagctcata tcagaggaag acctagggta gggcgcgcca ttataagtaa
      241 atgcatgtat actaaactca caaattagag cttcaattta attatatcag ttattacccg
      301 ggaatctcgg tcgtaatgat ttctataatg acgaaaaaaa aaaaattgga aagaaaaagc
      361 ttcatggcct ttataaaaag gaactatcca atacctcgcc agaaccaagt aacagtattt
      421 tacggggcac aaatcaagaa caataagaca ggactgtaaa gatggacgca tagatccggc
      481 ggccgctcta gaactagtgg atccccatgg tgatccataa cttcgtatag catacattat
      541 acgaagttat agatccggta cctggaggat gaggagacag aagttagtag taacaatttg
      601 gggacagaag aggatacagt gacatcagag gaagaagaca gcgaaggtat agtcaaaaag
      661 caataggaca gcaagacaat ttatttattt aatagttttt tataaatgta aaatctatca
      721 caacatgtgt acttaatttt ctgtggtagg ggaggccatt tcgttgaaac gtgtcgcgag
      781 cggttgtgag aattgactca ttttgattat tacgcaacca ttgaaatttt ttattttttt
      841 ttggtgaaga tttttcccac acaacttctt cttttactta accaccaact accgataatg
      901 gatttagtca atcatttacc agaccgtttg ttatttgctg ttcctaaaaa gggcagatta
      961 tacgaaaaat gctgtaactt attgagtggt gccgatatac agtttagaag atctaataga
     1021 ttagatatag cactttctac aaacttgcca attgcattaa tcttcttgcc tgcagctgat
     1081 atcccagttt tcgttggaga aggtaattgt gacttgggta taactgggtt agaccaaatc
     1141 aaagaagctg aacaattcga caacatcgag gacttgttgg atttgaaatt tggttcatgc
     1201 aaattgcaga tccaagttcc agcagatggc gagtacgaaa agccagaaca gcttgttgga
     1261 aagaaaattg tgtcttcatt tacaaaattg agtaccgact atttcaaaca attgtcagac
     1321 aaacctacta atatcagata tgtcggtggt tccgttgagg cttcttgtgc cttgggtgtt
     1381 gctgatgcta ttgtcgattt ggttgaaagt ggtgaaacta tgaaagcagc tgggttaaag
     1441 gccatagaaa ccatattgga gacttcggct catttgattt cgtcaaagaa aagtaaattc
     1501 ccagaaatgg tcaatataat cgtccaaaga cttcaaggtg ttttagctgc ccaggaatat
     1561 gtcttgtgta actacaatgc cccaaaatcc atccaagcaa aatgtttaac cattactcca
     1621 ggcagaaggg ccgctactgt ctctactttg gacaaacaca gcgacgacga agaggactgg
     1681 gttgccatct catccatggt taatagaaag gaaatcggta atgtaatgga cgaattgaag
     1741 aaagctggtg caaccgatat attggtactc gagatatcaa attgtagagt ttaaaagaag
     1801 tgatagtttc tcataaatat tgctagccca tggataactt cgtatagcat acattatacg
     1861 aagttattag ggataacagg gtaatctgca ggaaaaacga gctcgaattc atcgatgata
     1921 tcagatccac tagtggccta tgcggccgcg gatctgccgg tctccctata gtgagtcgta
     1981 ttaatttcga taagccaggt taacctgcat taatgaatcg gccaacgcgc ggggagaggc
     2041 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2101 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2161 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2221 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2281 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2341 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2401 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     2461 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2521 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2581 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2641 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     2701 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2761 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2821 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2881 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     2941 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3001 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3061 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3121 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3181 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3241 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3301 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3361 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3421 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3481 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3541 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3601 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3661 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     3721 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3781 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     3841 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     3901 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     3961 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     4021 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     4081 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     4141 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     4201 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgga catattgtcg
     4261 ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga
     4321 cactata
//
